Professional Documents
Culture Documents
Purpose
Centrifuges apply centrifugal force to separate sus-
pended particles from a liquid or to separate liquids of
ultracentrifuge. Variations of the high- and low-speed manual models, manufacturers typically supply tables
centrifuges found in the clinical laboratory include that permit easy determination of the RCF for a rotor
tabletop units, floor-standing models, and such spe- at a particular speed; microprocessor-controlled mod-
cial-purpose instruments as blood bank centrifuges, els automatically calculate the required rotational
microcentrifuges, microhematocrit centrifuges, cyto- speed and drive the motor.
centrifuges, and continuous-flow systems.
Centrifuge components
Low-speed centrifuges have a general operating
range of up to 10,000 revolutions per minute (rpm). The basic components of a blood bank centrifuge
Available in nonrefrigerated or refrigerated versions, include an electric motor, a shaft, and a rotor head
low-speed units are used primarily to centrifuge red (often interchangeable). The rotor, which holds the
blood cells or bulky precipitates; they cannot reach vessels of liquid, is spun at selected speeds by a motor.
sufficient speeds to adequately separate ultrasmall The rotor is housed within a chamber; a safety shield
particles (e.g., viruses, DNA molecules) or macromole- in the chamber surrounds the rotor as a precaution
cules or to perform density gradient centrifugation. against a broken head or similar failure, which could
hurl fragments at dangerously high velocities.
Blood bank centrifuges operate at low speeds (2,400
to 5,500 rpm). They are used to centrifuge solutions of Most blood bank centrifuges have a timer connected
suspended red blood cells, enhancing agglutination to the motor that allows timed use, while some models
and promoting the formation of packed cells at the can be operated in a continuous mode. Because of the
bottom of the tube — the cell “button” — which re- high operational speeds, the brushes often used to
mains during the decantation step of manual cell conduct electricity to the rotating armature of the rotor
washing. The centrifuge usually has a removable plas- can wear quickly and must be replaced more fre-
tic rotor that holds standard 10 × 75 mm or 12 × 75 mm quently than any other component. Some centrifuge
tubes. models use a frequency-controlled induction method
instead of brushes to conduct electricity to the drive
assembly. Many centrifuges come equipped with me-
Principles of operation chanical brakes, which press on the rotor itself, or
Centrifugation electric brakes, which reverse the polarity of the cur-
rent to the motor.
Centrifugation is based on the fact that an object
moving in a circular path at a steady angular velocity Rotor
is subjected to an outwardly directed force. The mag-
Samples to be centrifuged are typically loaded into
nitude of this force depends both on the rotor radius
tubes, cups, bottles, microscope slides, flasks, micro-
and the speed squared. This force is called the relative
plates, and racks, which are placed in slots on the rotor
centrifugal force or relative centrifugal field (RCF). It
head. Rotors are constructed from aluminum alloys for
is expressed as a multiple of g. RCF is used to compare
low to moderate speeds or titanium for high speeds and
forces attained by different centrifuges. Centrifuge
are available in a variety of designs, the two most
comparisons can be made in terms of RCF because the
common being the fixed angle and the swinging bucket
RCF describes the true separation efficiency of a cen-
(also called horizontal).
trifuge. In general, the RCF values of a standard blood
bank centrifuge range from 500 to 1,000 g (some range The fixed-angle rotor (see Fig. 1) is designed with 4
up to 4,000 g), while those of a standard tabletop to 40 holes at an angle between 20° and 45° to the
centrifuge range from 1,200 to 6,240 g. vertical axis of the rotor. These high-capacity rotors
are most often used for applications requiring total
Calculating the RCF exerted on a sample requires
sedimentation of a specimen component.
that the sample be located at a fixed distance from the
center of rotation. Due to rotor design, this distance The swinging-bucket rotor (see Fig. 2) commonly
varies from the top to the bottom of the sample holder, includes two to six freely moving buckets arranged
and the RCF exerted on the top of the sample may be symmetrically around the shaft. At rest, the buckets
significantly different from that exerted on the bottom. hang vertically; during operation, they swing out to a
The average of the values exerted on the top and 90° angle. This rotor can be used for incomplete sedi-
bottom of the same chamber, called average RCF, can mentation of a sample through a density gradient.
be used to compensate for this difference.
The zonal rotor is a large-capacity rotor shaped like
The RCF is calculated manually or automatically a bowl and divided by a veined core or by septa (see
and depends on the radius of the rotor head. For Fig. 3). Unlike conventional centrifugal rotors, the
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Centrifuges, Blood Bank
Other features
Other basic features include a power switch, brak-
ing device, speed control (potentiometer), timer, and
tachometer. Some units have a switch that allows
continuous operation or operation by a timer. Many
centrifuge models have a timer connected to the motor
for timed or continuous use. Some have a momentary-
or short-spin button that provides rapid acceleration
and high-speed centrifugation as long as the button is
pressed. Usually, it is possible to spin the rotor back-
ward or reverse its direction by holding the electric Figure 2. Swinging-bucket rotor
brake longer than is necessary to stop the motor.
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 3
Healthcare Product Comparison System
external lids, inner safety lids that fit over the rotor
and bucket or tube covers that contain the sample
material are available. Both safety lids and bucket/
tube covers help prevent exposure to aerosolized
pathogens. Gaskets provide a tight seal between the
unit lid and body to prevent expulsion of hazardous
materials and aerosols. Bowl drains have been added
to many models so that specimen spills and container
debris can be effectively and safely removed. Proce-
dures and personal protective equipment, such as gog-
gles and face shields, should also be used to minimize
user exposure to aerosolized bloodborne pathogens.
Components of centrifuges that come in contact with
hazardous materials and the operator should be prop-
erly disinfected (e.g., with 10% hypochlorite). Safety
issues have been addressed by several international
agencies (see Standards and Guidelines below).
Reported problems
Figure 3. Zonal rotor
Calibration is critical to the optimal performance of
centrifuges. Rotational speed and timer function
should be regularly checked and the appropriate com-
Centrifuge use
ponents recalibrated. If possible, the rotational speed
Operator access is provided by a lid, which usually measurement should be verified using a photo-
features a safety interlock to prevent the centrifuge tachometer under actual operating conditions (with
from operating while the lid is open, or a power discon- the centrifuge lid closed); however, not all centrifuges
nect, which automatically turns off the power to the readily allow calibration in this manner, and another
rotor when the lid is opened. Operator controls are technique may be required. Timers should be checked
located on the outside of the chamber or on a control with a stopwatch.
panel. Monitors commonly display the critical parame-
Because centrifuges are in constant use in the labo-
ters at which the unit is operating, such as rotational
ratory and because of their inherent operating haz-
speed (in rpm), temperature, and elapsed time.
ards, most manufacturers recommend frequent
The operator begins the centrifugation process by preventive maintenance. Leakage current and ground-
loading the samples into the rotor head within the ing resistance, as well as brushes, bearing lubrication,
instrument housing and closing the lid. Next, the op- and the safety interlock systems on lids, should be
erator selects the appropriate rpm, temperature (if the checked frequently. For safe operation and longer life,
unit is refrigerated), and total time for the desired brushes should be replaced before excessive wear oc-
separation, usually by flipping a switch (if only two curs, and loose carbon deposits should be cleaned regu-
settings exist), by adjusting a potentiometer, or by larly (see the citation from the Health Devices
using a keyboard. Some units also permit selection of Inspection and Preventive Maintenance System below).
the degree of braking applied to the shaft following
Centrifuges draw a large amount of current, result-
centrifugation.
ing in power line transients and electrical noise. Un-
Some centrifuge microprocessors permit the user to der these conditions, analytic instruments such as
program a set or sequence of operating parameters spectrophotometers, clinical chemistry analyzers, and
that are frequently used in laboratory procedures, such hematology analyzers can be particularly prone to
as acceleration rate, rotational speed, temperature, unreliable operation. Therefore, centrifuges should be
total time, and braking rate. After the operator loads connected only to circuits reserved for nonanalytic
a rotor head filled with patient samples, some models equipment.
allow the operator to select a coded program number
Centrifuge rotors are especially vulnerable to dete-
so that all steps in the procedure occur automatically.
rioration caused by foreign-matter buildup and should
When working with hazardous materials, personnel be regularly inspected and cleaned. Rotors should be
safety should be considered. In addition to the centrifuge’s replaced periodically; otherwise, they could fail and
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Centrifuges, Blood Bank
possibly destroy the centrifuge in the process. Rotors capabilities or purchasing a less expensive model that
must fit properly on the centrifuge because wobbles cannot handle the laboratory workload or is inade-
will cause excessive wear of the drive elements; if the quate for certain procedures. For example, larger fa-
rotor is not securely fastened, it may fly off at extraor- cilities where many cell-suspension samples are
dinary speed. Rotors should not be interchanged centrifuged daily may require a centrifuge with pro-
among different models unless they are specifically grammable functions. On the other hand, smaller labo-
designed to be interchangeable. Corrosive chemicals ratories that perform low or moderate daily sample
found in certain cleaning fluids can cause pitting or processing may not need a programmable unit.
etching of the rotor surface, so only manufacturer-
specified materials should be used for rotor cleaning Safety features are a prime consideration when
and maintenance. The recommended frequency of pre- purchasing a centrifuge. A centrifuge should have a
ventive maintenance varies among system compo- securely interlocking lid that either shuts off the unit’s
nents. Many manufacturers recommend daily bowl motor when the lid is opened or keeps the lid latched
cleaning. until the rotor has stopped. Additional safety features
include an inner protective cover over the rotor assem-
Centrifuges should never be operated with lids open bly and a display warning that the centrifuge should
because of the hazards posed by a flying rotor or the not be opened while the rotor is spinning. A centrifuge
dispersal of biologically hazardous material. ECRI re- should have clear, easily read visual indicators or
ceived a report (see the Health Devices citations below) displays; audible signals or alarms for device malfunc-
of a case in which a centrifuge tube broke during use tion, rotor imbalance, or an open lid are also helpful.
and blood products and glass sprayed from beneath the
lid onto the chest of a medical technologist, exposing
the worker to injury and possible infection. Tube
breakage can occur in centrifuges when the rotor is Stage of development
misaligned, tubes are old or defective, or the device is
Because centrifuge use can save time and increase
not used correctly. ECRI recommends using centri-
productivity compared to manual separation, demand
fuges with safety lid latches and/or interlocks that shut
for the devices has increased in recent years. By in-
off the motor when the lid is opened or that keep the
stalling two or more units, laboratories can cope with
lid latched until the rotor has stopped. In addition, the
the varying needs of discrete workstations, handle
unit should have an inner lid that can be securely
staggered workflow more easily, and efficiently handle
fastened.
large or small specimen batches. The large variety of
Operators should be aware that excessive vibrations rotor heads available permits efficient sharing of the
(often called “criticals”) occur at certain rotational same instrument among several workstations.
speeds particular to each instrument. This dynamic
condition, known as resonance, is induced by the com- The recent trend in tabletop centrifuge design has
bination of the mechanical vibrations of the moving been toward brushless motors. Some centrifuges fea-
parts. Centrifuges should be operated at speeds ture longer-wear brushes, while other models do not
slightly above or below resonant speeds to avoid exces- use brushes at all. The advantage of using a brushless
sive wear. induction system is that this method involves no physi-
cal contact with the motor; the system uses a magnetic
Operators should always use universal precautions field to induce the electrical current.
when performing centrifugation and other functions that
may cause exposure to splashed blood or bodily fluids. In addition to brushless induction systems, newer
These precautions include wearing disposable gloves, centrifuges may have permanently lubricated bear-
facial protection (e.g., shields), gowns or laboratory ings, microprocessors that allow the operator to select
coats, and plastic aprons as specified by NCCLS — The operational parameters by keyboard, and LED dis-
Clinical Laboratory Standards Organization and the plays of operational parameters. Some microproces-
Occupational Safety and Health Administration sor-controlled centrifuges can identify their rotors by
(OSHA) (see Standards and Guidelines below). reading a bar code imprinted on the rotor surface; the
microprocessor can then set operational parameters on
centrifuge operation by using the bar-code information
Purchase considerations to access data stored in memory (e.g., maximum
Buyers should try to correlate blood bank centrifuge speed). Computerized centrifuges can interface with
features with their current and projected laboratory personal computers to record and/or modify opera-
needs to avoid overspending on a unit with unnecessary tional parameters.
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Healthcare Product Comparison System
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Centrifuges, Blood Bank
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Healthcare Product Comparison System
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Centrifuges, Blood Bank
Sigma
Kendro Laboratory Sigma Laborzentrifugen GmbH [160990]
Postfach 1713
Kendro Laboratory Products [351427] An der Unteren Soese 50
31 Pecks Ln D-37507 Osterode am Harz
Newtown CT 06470-2337 Germany
Phone: (800) 522-7746 Phone: 49 (5522) 50070
Fax: (203) 270-2166 Fax: 49 (5522) 500712
E-mail: info@kendro.com E-mail: info@sigma-zentrifugen.de
Internet: http://www.kendro.com Internet: http://www.sigma-zentrifugen.de
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 9
Healthcare Product Comparison System
About the chart specifications Note: The data in the charts derive from suppli-
ers’ specifications and have not been verified through
The following terms are used in the chart:
independent testing by ECRI or any other agency.
Rotational speed: The maximum values of rotational Because test methods vary, different products’ specifi-
speed and centrifugal force available, in units of rpm cations are not always comparable. Moreover, products
and RCF, respectively, along with the number of and specifications are subject to frequent changes.
speed settings available. ECRI is not responsible for the quality or validity of
Abbreviations: the information presented or for any adverse conse-
quences of acting on such information.
ASTM — American Society for Testing and Materials
When reading the charts, keep in mind that, unless
CE mark — Conformite Europeene mark otherwise noted, the list price does not reflect supplier
CSA — Canadian Standards Association discounts. And although we try to indicate which
features and characteristics are standard and which
dB — Decibel are not, some may be optional, at additional cost.
dBA — Decibel (A scale) For those models whose prices were supplied to us
in currencies other than U.S. dollars, we have also
GS — Gepruefte Sicherheit
listed the conversion to U.S. dollars to facilitate com-
IEC — International Electrotechnical Commission parison among models. However, keep in mind that
exchange rates change often.
ISO — International Organization for Standardiza-
tion Need to know more?
LCD — Liquid crystal display For further information about the contents of this
LED — Light-emitting diode Product Comparison, contact the HPCS Hotline at +1
(610) 825-6000, ext. 5265; +1 (610) 834-1275 (fax); or
MDD — Medical Devices Directive hpcs@ecri.org (e-mail).
10 ©2003 ECRI. Duplication of this page by any means for any purpose is prohibited.
Centrifuges, Blood Bank
TIMER
Range, min 0-999 0-99, 59 sec 0-99, 59 sec 0-6,000, hold
Out-of-time alarm End of run End-of-cycle alarm End-of-cycle alarm End-of-run tone
ACCELERATION
Sec to maximum 9 5-12 5-12 15 sec to 10 min
BRAKING
Sec from maximum 9 30, fully loaded 30, fully loaded Variable
POWER REQUIREMENTS,
VAC 230/240 115/230, 50/60 Hz 115, 50/60 Hz 208/220/240
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 11
Healthcare Product Comparison System
WEIGHT, kg (lb) 340 (749.7) 12.7 (28) 12.7 (28) 253.1 (558)
PURCHASE INFORMATION
List price $16,000 $1,672 (115 VAC), $1,767 $23,900
$1,334 (230 VAC)
Delivery time, ARO 60 days From stock From stock 30-45 days
Fiscal year January to December October to September October to September January to December
OTHER SPECIFICATIONS 99 programs; 1-speed unit; 2-speed unit; 25 A circuit; JE 5.0
multistage program onboard tachometer; onboard tachometer; Elutriator; vacuum
consisting of 9 digital keypad/ digital keypad/ operation at 0.5
programs with up to display. Meets re- display. Meets re- atmosphere;
8 stages and quirements of CSA, quirements of CSA, electronic calibra-
different sets of ISO 9001, and UL. ISO 9001, and UL. tion circuit;
parameters; analog controls.
precooling program; Meets requirements
integral setting of CSA, ISO 9001,
with variable rotor- and UL.
radius setting.
12 ©2003 ECRI. Duplication of this page by any means for any purpose is prohibited.
Centrifuges, Blood Bank
ROTOR
Max tubes, 10x75 mm 222 (12 x 75) 222 (12 x 75) 12 Not specified
Removable Yes Yes Yes Not specified
Construction Aluminum Aluminum Aluminum, PVC Aluminum
TIMER
Range, min 0-6,000, hold 0-6,000, hold 0-99, 59 sec 0-30
Out-of-time alarm End-of-run tone End-of-run tone End of step End-of-run bell
ACCELERATION
Sec to maximum 15 sec to 10 min 15 sec to 10 min Not specified 15
BRAKING
Sec from maximum Variable Variable 60 15-20
POWER REQUIREMENTS,
VAC 208/220/240 208/220/240 220/240, 50 Hz, 1 A 120
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 13
Healthcare Product Comparison System
PURCHASE INFORMATION
List price $25,900 $28,000 A$1,350 (US$803) $1,895
14 ©2003 ECRI. Duplication of this page by any means for any purpose is prohibited.
Centrifuges, Blood Bank
ACCELERATION
Sec to maximum 26 26 13 Not specified
BRAKING
Sec from maximum 19 19 Not specified 45
POWER REQUIREMENTS,
VAC 115/220, 50/60 Hz 115/220, 50/60 Hz 115, 60 Hz; 115, 60 Hz;
230, 50 Hz 230, 50 Hz
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 15
Healthcare Product Comparison System
PURCHASE INFORMATION
List price $1,795 $4,495 Not specified Not specified
Delivery time, ARO Not specified Not specified 7-10 business days 7-10 business days
Year first sold Not specified Not specified Not specified 2003
Fiscal year Not specified Not specified January to December January to December
OTHER SPECIFICATIONS All parameters can All parameters can Large size enables 120 V. Meets
be locked to avoid be locked to avoid unit to accept a requirements of CSA.
unintentional unintentional variety of clinical
changes; brake can changes; 2 program and industrial
be deactivated for buttons for quick laboratory
sensitive blood recall of para- accessories.
separations; meters; temp range
separate green-lit -9° to 40°C;
standby button; rpm fast-cool function
to g-force display. obtains set temp;
Meets requirements brake can be deacti-
of IEC 1010-02-02 vated for sensitive
safety standards and blood separations;
UL approved. at set rpm function,
begins timer when
nominal speed is
reached; rpm to
g-force display.
Meets requirements
of IEC 1010-02-02
safety standards and
UL approved.
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Centrifuges, Blood Bank
ACCELERATION
Sec to maximum 15 10 30 100
BRAKING
Sec from maximum NA 25 34 160
POWER REQUIREMENTS,
VAC 115, 60 Hz 115 220-240, 50 Hz 400, 50 Hz, 3-phase
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 17
Healthcare Product Comparison System
WEIGHT, kg (lb) 5.9 (13) 18.1 (40) 172 (379.3) 264 (582.1)
PURCHASE INFORMATION
List price $595 Not specified Not specified Not specified
Delivery time, ARO 7-10 business days 7-10 days Not specified Not specified
Fiscal year January to December January to December January to December January to December
OTHER SPECIFICATIONS Electrical-interlock See-through lid; Brushless induction Brushless induction
lid for safety. spill-resistant drive; rotor recog- drive; rotor recog-
Meets requirements control panel. nition; microproc- nition; microproc-
of CSA. essor controlled; essor controlled;
pulse button; pulse button;
several run-up and several run-up and
braking choices; braking choices;
time controlled; time controlled;
automatic RCF calc- automatic RCF calc-
ulation; 89 user- ulation; 89 user-
defined programs; defined programs;
precooling system; precooling system;
RCF integrator. RCF integrator.
Meets requirements Meets requirements
of IEC 1010-1. of IEC 1010-1.
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Centrifuges, Blood Bank
TIMER
Range, min 1 sec-99 min, 59 sec 0-999, hold 0-999 0-99 min, 59 sec,
hold
Settings, increment 1 sec 1 sec 1 sec 1 sec
ACCELERATION
Sec to maximum 120 Depends on rotor Depends on rotor Not specified
BRAKING
Sec from maximum 120 Depends on rotor Depends on rotor Not specified
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 19
Healthcare Product Comparison System
WEIGHT, kg (lb) 300 (661) 340 (750) 490 (1,080) 307 (678)
PURCHASE INFORMATION
List price Not specified Not specified Not specified Not specified
Fiscal year Not specified January to December January to December Not specified
OTHER SPECIFICATIONS Brushless motor Optional fitted None specified. ACE feature; sealed
emits no dust; Jouan electronic covers for biocon-
accepts MAP bags quality control tainment; LCD; 3-
(ACD-A quadruple and data collection position keylock;
bags); programmed system. programmable slow
memory operation; start/stop; optional
timer controls net Watchdog Network
running time; con- Plus software,
venient built-in BarTrace bar-code
functions; variety scanner.
of safety devices
and self-diagnosis
functions. Meets re-
quirements of ISO
9001 and 14001.
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Centrifuges, Blood Bank
ROTOR
Max tubes, 10x75 mm 396, H-6,000 A H-12,000 420 80
Removable Yes Yes Yes Yes
Construction Stainless steel Stainless steel Aluminum alloy Steel
body, aluminum body, aluminum
buckets ** buckets
OTHER ROTORS AVAIL 15 No Swing, angle Yes
Tubes @ sizes/vol Not specified Not specified 10, 15, 50, 100, 0.2-250 mL
500 mL
TIMER
Range, min 0-30, hold 0-99 min, 59 sec, 1-99, hold 30, hold
hold
Settings, increment 1 sec 1 sec Variable 1 sec or hold
ACCELERATION
Sec to maximum Not specified Not specified 2 min 30 sec 20 sec/2 curves
BRAKING
Sec from maximum Not specified Not specified 3 min 28 sec/2 curves
©2003 ECRI. Duplication of this page by any means for any purpose is prohibited. 21
Healthcare Product Comparison System
PURCHASE INFORMATION
List price Not specified Not specified Y2,550,000 €3,800 (US$4,120)
(US$24,088) without with 1 set of
rotor accessories
Warranty 1 year 1 year 1 year 18 months
Fiscal year Not specified Not specified July to June January to December
OTHER SPECIFICATIONS Automated RCF Plus software for PC Microprocessor High-speed angle
determination; communications; ACE controlled; rotors. Meets
microprocessor con- feature; LCD; 3- refrigeration requirements of
trol; W2DT; slow position keylock; available; brushless GS and ISO 9001.
start/stop; sealed program 3-position motor. Meets
covers for bio- keylock; program- requirements of
containment; mable slow start/ ISO 9001.
programmable; LCD. stop; optional
Watchdog Network,
BarTrace bar-code
scanner.
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Centrifuges, Blood Bank
ROTOR
Max tubes, 10x75 mm 10 (12 x 75)
Removable Yes
Construction Plastic
TIMER
Range, min 0-3
ACCELERATION
Sec to maximum 8
BRAKING
Sec from maximum 15
Type Electric
BRUSH/BRUSHLESS Brushless
ALERT INDICATORS None
POWER REQUIREMENTS,
VAC 120
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Healthcare Product Comparison System
PURCHASE INFORMATION
List price $975, including
rotor
Warranty 1 year
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