Mallari, Keith Charlotte M.

BSN 1-C (GROUP 7)

A. Draw a Simple Microscope and Label the Parts Properly (print the labels).
B. Complete the table. (Using the table below list down parts of the microscope, describe
each component and indicate its function/s)

PARTS OF THE MICROSCOPE DESCRIPTION/FUNCTIONS

Ocular lens or eyepiece Topmost part of the microscope which is the

lens the viewer looks through to see the
specimen.
Revolving Nose Piece Located above the stage, it holds the objective
lenses.

Body Tube It connects the eyepiece to the objective

lenses.
Coarse Focus It brings the specimen into general focus.

Fine Focus It fine-tunes the focus and increases the

details of specimen.
Arm It connects the body tube to the base of
microscope.

Iris Diaphragm Found on the condenser, it is used to adjust

the amount of light coming through the
condenser.
Illuminator The light source of microscope.

Stage Located beneath the revolving nosepiece, it is

the platform on which the specimen is placed.

Stage Clips Situated above the stage, these are metal

clips that hold the slide in place.
Base It supports the microscope and it is where the
illuminator is found.

Lower Power Objectives Low power objectives cover a wide field of

view and they are useful for examining large
specimens or surveying many smaller
specimens. This objective is useful for aligning
the microscope. The power for the low
objective is 10X. Place one of the prepared
slides onto the stage of your microscope.
Middle Power Objectives It increases the magnification.
On/off switch
This switch on the base of the microscope
turns the illuminator off and on.

High Power Objectives A high-power objective lens magnifies 40x,

with total magnification 400x if the eyepiece
lens is 10x power, and it is ideal for observing
very fine detail, such as nerve cells in the
retina or the striations in skeletal muscle.
TYPES OF DESCRIPTION IMAGES OF IMAGES OF SPECIMEN
MICROSCOPE MICROSCOPE
The Compound
Microscope is a type
of microscope that
contains more than
one magnifying lens.
It can magnify
objects
approximately a
COMPOUND
thousand times their
MICROSCOPE original size. Visible
light is its main
source of
illumination. As such,
it is also known as
the compound light
microscope.

Made up of a series
of lenses and
utilizing visible light
as its source of
illumination, the
brightfield
microscope can
BRIGHTFIELD
magnify an object
MICROSCOPE 1,000 to 1,500 times.
This is used to
visualize bacteria
and fungi. Objects
less than or thinner
than 0.2 um cannot
be visualized by this
type of microscope.
 An inverted
microscope allows
the user to place the
petri dish on a flat
stage, with the
objective lenses
housed beneath the
stage. Inverted
microscopes are
used for in-vitro
fertilization, live cell
INVERTED imaging,
MICROSCOPE developmental
biology, cell biology,
neuroscience, and
microbiology.
Inverted microscopes
are often used in
research to analyze
and study tissues
and cells, and in
particular living cells

Metallurgical
Microscopes are high
power microscopes
designed to view
samples that do not
allow light to pass
through them. It is
utilized in the
METALLURGICA aerospace industry,
L MICROSCOPE the automobile
manufacturing
industry, and by
companies analyzing
metallic structures,
composites, glass,
wood, ceramics,
polymers, and liquid
crystals.
 It makes use of
ultraviolet light and
fluorescent dyes
called fluorochomes.
The specimen under
study fluoresces or
appears to shine
against a dark
background.
Fluorescence
microscopy is based
FLUORESCENCE on the principle that
MICROSCOPE certain materials emit
energy that is
detectable as visible
light when they are
irradiated with the
light of a given
wavelength. It uses
higher intensity of
light source and this
in turn excites a
fluorescent species.
It may also be used
to visualize the
genetic material of
the cell (DNA &
RNA).

It is based on the
principle that
differences in
refractive indices and
light waves passing
through transparent
objects assume
different phases. The
PHASE-
phase-contrast
CONTRAST
microscope has a
MICROSCOPE
contrast-enhancing
optical technique in
order to produce
high-contrast images
of specimens that
 are transparent
which include thin
tissue slices, living
cell in culture and
subcellular particles
(such as nuclei and
organelles).

Electron microscopy
(EM) is a technique for
obtaining high
resolution images of
biological and non-
ELECTRON biological specimens. It
MICROSCOPE is used in biomedical
research to investigate
the detailed structure
of tissues, cells,
organelles and
macromolecular
complexes.

The scanning probe

microscope was
developed in the 1980s
by the Swiss Scientists
Dr. Gerd Binnig and
Dr. Heinrich Rohrer. It
is used to study the
molecular and atomic
shapes of organisms
on a nanoscale. A
SCANNING physical probe is used
PROBE to scan back and forth
MICROSCOPY over the surface of a
(SPM) sampe. A computer
then gathers data that
are used to generate
an image of the
surface. It can also be
used to determine the
variations in
temperature inside the
cell as well as its
chemical properties.