HPLC / BIOCHEMICAL

S E P A R A T I O N
Xanthines
Human Serum Profile
on Brownlee Pecosphere 5 C18

Column: Brownlee Pecosphere 5 C18

N O T E
Peak List Column
1. creatinine Column name: Brownlee Pecosphere 5 C18
2. uric acid Part number: 02580169
3. tyrosine Dimensions: 150 x 4.6 mm
4. hypoxanthine Particle size: 5 µm
5. uridine
6. xanthine Conditions
7. inosine Mobile phase: A: 60% MeOH/H2O
8. guanosine B: 0.02 M NaH2PO4 in water, pH 5.6
9. tryptophan Flow: 2.0 mL/min
10. theobromine Gradient: 0.5-40% A in 7 min
11. caffeine Detector: UV @ 254 nm

Sample
Injection volume: 10 µL of deproteinized serum

PerkinElmer Life and

Analytical Sciences
710 Bridgeport Avenue
Shelton, CT 06484-4794 USA
Phone: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices

©2006 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. All other trademarks not owned by PerkinElmer, Inc. or its
subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability
for editorial, pictorial or typographical errors.

007568_23