Professional Documents
Culture Documents
PRACTICAL APPLICATIONS
The sufu lexicon helps product developers, researchers, and technologists
understand the characteristics of plain sufu and those positive attributes when
developing new, similar products and investigating quality-control and shelf-life
topics. Because all mature plain sufus examined here presented similar sensory
profiles derived from their major attributes, future studies could focus on distinct
fermentation periods used to optimize products by employing the available
definitions and references. Moreover, the developed lexicon can serve as a tool for
correlating descriptive data to consumer preferences and to facilitating an
understanding of the attributes that drive consumers’ acceptance.
INTRODUCTION
tofu cubes are covered with thick and white fungal mycelia
Sufu (fermented soybean curd) is a cheese-like product that that contain proteinases. In the third stage, moldy tofu cubes
is commonly an element of traditional Chinese cuisine (pehtzes) are embedded in a large amount of table salt to
(Yasuda 2011). The development of this product can be extract the water present inside the cubes. In the final stage,
dated back to >300 years ago in Ch’ing dynasty, China (Rui which is also referred to as the postfermentation or aging
2009; Yasuda 2011), and it is now widely consumed as an process, salted pehtzes are submerged in a mixture of rice
appetizer or side dish in China. The typical ingredients of wine and brine solution in containers and aged for 3–6
plain sufu include soybean, rice wine, water, salts, and ses- months before consumption (Chung et al. 2005; Ma et al.
ame oil (Rui 2009) and its manufacturing process can be 2013).
divided into 4 stages. In the first stage, tofu (soybean curd) Proteolysis plays a crucial role in producing acceptable
is produced. In the second stage, which is also called prelimi- sufu product during the final aging stage (Li et al. 2010; Moy
nary fermentation, freshly cut tofu cubes (roughly 2.5 3 2.5 and Chou 2010). Glycinin (7S), one of the major storage
3 1.0 cm) are inoculated with sufu fungus and incubated proteins in soybeans, is partially hydrolyzed to peptides,
under appropriate conditions (relative humidity: 71–73%; amino acids and other small nitrogenous compounds such
temperature: 20–248C) for 48–60 h. At the end of this stage, as amines during aging (Yasuda 2011). These derived
compounds contribute to the taste of sufu (Guan et al. (Cherdchu et al. 2013). Other aroma descriptors such as
2013). Large quantities of glutamic acid and aspartic acid “alcohol,” “soy sauce,” and “fermented fish” were used by
were reported in sufu and likely contribute to the umami Korean panelists to describe the attributes of Doenjang, a
taste (Li et al. 2010), but no other taste peptides such as Korean soybean paste condiment (Kim et al. 2010).
aspartame were reported. Frequently, sufu is described as a cheese-like product, so
Similar to cheese, variation in salt content in sufu may texture descriptors such as hardness, cohesiveness, and tooth
contribute to their texture differences. It is found that the packing for profiling cheese provide clues for developing a
salt content in sufu not only provides a salty taste, but also similar lexicon for sufu texture (Barden et al. 2012; Poltorak
controls its enzymatic activity (Ma et al. 2013) and influen- et al. 2015). In the case of the taste attributes of soybean-
ces protein degradation (Guan et al. 2013). As a result, an based fermented products, descriptors such as “salty,”
increase in the salt content could raise the degree of hardness “umami,” and “sweet” were developed to describe soy sauce
and elasticity but decline the degree of adhesiveness of sufu, by panelists from Korea, Thailand, and U.S.A. (Cherdchu
whereas a reduction in the salt content could result in et al. 2013; Cherdchu and Chambers 2014), whereas the
diminished hardness and elasticity but an increased level of descriptors “salty” (taste), “MSG” (taste), “alcohol” (flavor),
protein and lipid degradation (Xia et al. 2014). “soy sauce” (flavor), and “roasted soybean” (flavor) were
Previous research has studied another ingredient, rice wine. developed for Doenjang (Kim et al. 2010).
Li et al. (2010) found that the alcohol in the wine inhibited For capturing the characteristics of plain sufu, a lexicon
the multiplication of microorganisms and slowed the activity must be developed that accurately describes its sensory pro-
of proteases. Chung et al. (2005) evaluated the volatile com- file. Therefore, the objectives of this study were to (1) iden-
pounds in mature plain sufu, and concluded that the pleasant tify and define the sensory attributes that characterize plain
sufu flavor was due to the formation of the aromatic esters sufu in terms of its aroma, texture, taste, and flavor and (2)
between alcohol and other small volatile compounds. compare sensory profiles among commercial products using
Studies involving the flavor of sufu have reported alcohols, the lexicon developed by trained panelists. This work could
acids, and esters to be the 3 major aromatic chemical groups, provide information essential for improving the quality and
and identified 17 potent odorants using gas chromatography-
production of plain sufu.
flame ionization detection-olfactometry (Chung et al. 2005).
The panel developed terms such as sour, sweet, fruity,
coconut-like, and meaty to describe these distinct odors. MATERIALS AND METHODS
Among alcohols, ethanol is intentionally added during the
production of sufus, but other alcohols such as 3-methyl-1- Sufu Samples
butanol, 2-butoxyethanol, and 2-ethyl-1-hexanol are produced
Twelve brands of plain sufu were purchased randomly from
during fermentation catalyzed by various fungal enzymes
local grocery stores in Hong Kong. The products differed in
(Chung 1999). In the case of acids, most of the fatty acids
their place of origin, ingredients, and moisture content
detected in the product are released from soybean lipids in the
(Table 1). All products had similar preparation dates and
presence of lipases (Chung et al. 2005). Moreover, acetic acid
preserved in sealed glass bottles. Each sufu cube shared anal-
is present in high concentration in both plain and red sufus
ogous size (2.0–3.0 cm long on 2 sides, 1.5–2.0 cm thick).
and has been reported to taste sour (Chung 1999; Chung et al.
2005). Esters, which were identified as the third largest aro- Selection of Panel for Lexicon Development
matic chemical class in sufu (Chung et al. 2005), are likely pro-
duced in the reaction between the acids and alcohols present Initially, 54 volunteers (students and staff from The Chinese
and yield the pleasant fruity and floral aroma (Chung 1999). University of Hong Kong) were prescreened using question-
To date, no single investigation has reported a compre- naires to evaluate their health status, enthusiasm level, and
hensive list of descriptors for plain sufus, except for the study availability (Meilgaard et al. 2006). All volunteers had previ-
conducted by Yang et al. (2002), who presented several ously eaten plain sufu, were familiar with it, and did not
attributes such as “sesame oil-like,” “alcohol-like,” “salty,” object to consuming it. They were firstly screened for their
and “umami.” However, the report lacked definitions of the abilities to discriminate among the 5 basic tastes and 5 odors
presented descriptors and quantitative references. A few lexi- by matching test. In the test, the 5 basic tastes aqueous solu-
cons for describing other soybean-based fermented products tions (15 mL), sweet (2% sucrose), salty (0.2% NaCl), sour
have been developed and studied in detail (Kim et al. 2010; (0.08% citric acid), bitter (0.7% caffeine), and umami (0.2%
Cherdchu et al. 2013; Cherdchu and Chambers 2014). For MSG), and 5 odors (1 drop each of food-grade flavor, placed
example, the aroma descriptor “fermented” was developed separately in a 10-cm3 sealed glass tube), peppermint, orange,
to describe soy sauce (Cherdchu and Chambers 2014), and it ginger, jasmine, and vanilla were provided in random order.
was confirmed by panelists from both Thailand and U.S.A. The volunteers who matched all the tastes and flavors
TABLE 1. TWELVE BRANDS OF PLAIN SUFUS, INGREDIENTS, AND used to facilitate discussion. Initially, the panel was provided
MOISTURE CONTENT three 2-h introductions (total 6 h) to practice using method-
Ingredients listed ology and terms based on our previous studies on sufu
Brands Origin* on the food label Moisture (%)† (Fung 2004) and on other published lexicons related to fer-
A-1 A soybean, rice wine, salt, 252 6 3d mented foods such as cheese (Barden et al. 2012; Poltorak
sesame oil, water, hot pepper et al. 2015) and soy sauce (Cherdchu et al. 2013). Subse-
A-2 A soybean, rice wine, salt, water 281 6 4f quently, to familiarize the panelists with the characteristics
A-3 A soybean, rice wine, salt, 295 6 10g or qualities (aroma, texture, taste, and flavor) of the sufus,
sesame oil, water
the 5 brands of sample cubes were served in random order
A-4 A soybean, rice wine, salt, water, 259 6 4de
A-5 A soybean, rice wine, salt, 278 6 11f
at room temperature (20 6 28C) and presented to each pan-
sesame oil, water elist. The panelists were instructed to evaluate samples in
A-6 A soybean, rice wine, salt, 281 6 8f this sequence: (1) smell and evaluate the aromas of the sam-
sesame oil, water ple placed inside the sampling cup; (2) cut the sample cube
B-7 B soybean, rice wine, salt, 226 6 3b into 2 halves using the provided small plastic spoon (1/
sesame oil 2 in.), scoop out a complete spoonful of the sample, and use
B-8 B soybean, rice wine, salt, water 242 6 6c
the tongue to feel the sample surface and expectorate; (3)
B-9 B soybean, rice wine, salt, 221 6 11b
sesame oil, water
take another spoonful of the sample, leave it on the upper
B-10 B soybean, rice wine, salt, 265 6 5e front surface of the tongue, compress it against the palate
sesame oil, water, hot pepper once, and evaluate its texture and expectorate; and (4) take a
B-11 B soybean, rice wine, salt, 236 6 11c third fresh spoonful of the sample, compress it against the
sesame oil, water palate 10 times, and then evaluate both texture and flavor. In
C-12 C soybean, rice wine, salt, water 177 6 6a each new session, the panelists were encouraged to present,
Notes: Significant differences of moisture among different sufu brands clarify, and discuss the descriptors for sufu that they devel-
were determined at P50.05 level by one-way ANOVA. Different let- oped in the same session.
ters (a, b, c, etc.) signify different levels between sufu brands. The lexicon was developed for the 5 samples over three
* A, B, and C, are for locations A, B, C, respectively.
new 3-h discussion sessions (total 9 h) according to
† Percentage on dry weight basis (w/w).
ISO11035 (1994), with modification in M value reduction.
Each sample was present in a sequential monadic order.
accurately were next screened by the ranking test designed for Briefly, the panelists were first asked to individually identify
(1) the 5 basic tastes prepared in aqueous solution (15 mL), the descriptors for each sample as specifically as possible,
sweet (2.0%, 5.0%, 10.0%, and 16.0% sucrose), salty (0.2%, then all of the descriptors were compiled into a list. Second,
0.35%, 0.5%, and 0.7% NaCl), sour (0.05%, 0.08%, 0.15%, the list was provided to each panelist for discussion and con-
and 0.20% citric acid), bitter (0.05%, 0.08%, 0.15%, and solidation: newly generated descriptors were added to the list,
0.20% caffeine), and umami (0.2%, 0.35%, 0.5%, and 0.7% incompletely understood descriptors were discussed further,
MSG) and (2) one texture cube (1/2 in. slice each), hardness: and similar descriptors were combined. Lastly, the panelists
Miracle Whip (Kraft Foods), peanut butter (Unilever), and scaled the intensity of the descriptors in the list using a 5-
cream cheese (Kraft Foods). In this test, those 6 sets of samples point line scale featuring 1.0-point increments (Pereira et al.
were served in random order within groups. The volunteers 2015). The results were collected and calculated using Eq. (1):
need to identify the differences in intensities among individual pffiffiffiffiffiffiffiffi
set and then ranked samples from the weakest to the strongest M5 F I (1)
according to their sensations. Assessors, whose ranking accu-
racy >80%, would be sorted out as potential panelists (Meil- where M is the geometric mean, the square root of F and I
gaard et al. 2006). After the two screenings, 16 volunteers for each descriptor; F is frequency of each descriptor
remained as panelists and received four 3-h training sessions expressed as a percentage (the number of times a descriptor
(total 12 h). During the sessions, the panelists were introduced is mentioned divided by the total number of times); and I is
to additional terminologies, references, and rating scales that the relative intensity of each descriptor expressed as a per-
centage (the sum of the intensities yielded by the entire panel
were used in the subsequent descriptive analysis, but before
for a given descriptor divided by the maximal possible inten-
the lexicon development.
sity for the descriptor).
The descriptors were ranked according to the M value.
Lexicon Development
Initially, 59 descriptors were collected, but the descriptors
From the original 12 brands of products, 5 brands were ran- whose M values below 3.0 were removed. The remaining 22
domly selected for lexicon development. A round table was descriptors formed the lexicon, and were refined through
TABLE 2. DEFINITION AND REFERENCE STANDARDS OF PLAIN SUFU AROMA FOR DESCRIPTIVE SENSORY ANALYSIS
Reference, name/brand
No. Attribute Definition (standard intensity)*
1 Soybean-like Aromatics associated with cooked 15 mL Fresh soymilk; Vitasoy San
soybean with beany smell, such Sui Soymilk Low Sugar† (6.0).
as soybean curd, soymilk.
2 Fermented A combination of aromatics that are 15 mL Fish sauce; Suree Original†
overripe, rotten, fermented fish (10.0).
sauce like.
3 Moldy Aromatics associated with mold 3.0 g Blue cheese; Danish† (10.0).
growth include sour and blue
cheese-like.
4 Salty Salty food smell, induced by high 15 mL Dark soy sauce; Gold label
concentrate of sodium chloride in Amoy† (12.0).
liquid such as dark soy sauce.
5 Alcohol-like Colorless pungent chemical-like 6.2 g Rice wine, alcohol at 29%
aromatic associated with distilled v/v; Pearl River Bridge† (10.0).
spirits of grain product, such as
the Chinese rice wine.
6 Fish-like Aromatics associate with fish and/or 5.0 g Steamed salted dried fish,
shellfish, such as dried fish, White Herring (Ilisha elongate)†
clams, etc. (10.0).
7 Sesame oil-like A combination of aromatics associ- 6.2 g Pure sesame oil; Lee Kum
ated with nutty, buttery, brown, Kee† (12.0).
woody characteristic of sesame
oil.
8 Meaty Cooked meat extracts and/or broth 4.0 g Boiled pork meat (4.0)‡.
without bloody, may or may not
have a Maillard brown character.
Notes:
* Intensity is based on a 15-point numerical line scale with 0.5 increments, where 0 means “none” and 15 means “extremely strong.”
† Bold characters indicate the brands/species of food used in this study.
‡ Fresh lean pork meat (PARKnSHOP, Hong Kong limited, Watson House) was prepared by boiling meat in portable water at the ratio of 1 g:2.5
mL water for 20 min.
further group discussion (Tables 2 and 3). Standards for dis- China) a full spoon of the sample in their mouth to evaluate
tinct intensities used to describe each attribute were initially both texture and flavor. For the sensory analysis, 7 panelists
selected by the panel leader from similar food systems. They (4 females and 3 males, aged between 20 and 30 years) who
then undergo group discussion, and were regarded as refer- demonstrated high repeatability and consistency in evalua-
ences after reaching consensus (Fung 2004; Meilgaard et al. tions were selected for descriptive sensory analysis.
2006). In the case of attributes for which no intensity stand- The experimental samples were evaluated over 20 days,
ards were available in the literature, new standards were during which 2 sessions were held per day, with a 5-h break
developed through group discussion. The reference samples between the sessions. Each brand of sample was evaluated 3
served as “anchors” in subsequent scaling of sufu samples times by each panelist on different days. Bottled sufu sam-
(Table 3). ples to be evaluated were stored at 48C and used within 7
days after opening. Before being evaluated, sample cubes of
Descriptive Sensory Analysis each brand were placed in tightly covered, disposable and
All panelists had prior experience in lexicon development for odor-free plastic portion cups (25 mL) for 15 min at room
other products, but before the experimental sessions, they temperature (20 6 28C); the panelists evaluated the samples
received training (in six 3.5-h sessions, total 21 h) in descrip- at the same temperature (20 6 28C) under normal florescent
tive sensory analysis of plain sufu. The panelists were lighting in the food preparation laboratory of the School of
instructed on the definition of attributes and trained to use a Life Sciences, The Chinese University of Hong Kong. During
15-point descriptive scale, and they were directed to first the evaluation, deionized carbon-filtered water, unsalted
smell a sample and then place (using a white disposable, crackers, or fresh carrot slices were provided to the panelists
odor-free plastic coffee spoon, volume 0.5 mL, Guangdong, as palate cleansers.
TABLE 3. DEFINITION AND REFERENCE STANDARDS OF PLAIN SUFU TEXTURE, TASTE AND FLAVOR FOR DESCRIPTIVE SENSORY ANALYSIS
Reference, name/brand (standard
No. Attribute Definition intensity)*
Texture
1† Roughness The amount of particles on the food Peanut butter, Unilever/Skippy‡ (1.0)
surface. Orange peels, peels from fresh
orange, inside surface (5.0)
Potato chips, Lay’s Classic‡ (9.0)
2† Wetness The amount of moisture, on the Cheese, yellow, American pasteur-
food surface. ized process deli‡, 1/2 in. slice
(2.0)
Strawberry Yogurt; Yoplait Original‡
(10.5)
3† Hardness Force to be used to compress foods Miracle whip; Kraft Foods‡ (5.0)
between tongue and palate Peanut butter; Unilever/Skippy‡
(11.0)
Cream cheese; Kraft/Philadelphia‡,
1/2 in. cube (14.0)
4† Adhesiveness The effort to completely remove Strawberry Yogurt; Yoplait Original‡
samples from the palate (4.0)
Peanut butter; Unilever/Skippy‡ (9.8)
5† Cohesiveness The degree to which sample Chiffon cake; Garden‡ (1.0)
deforms rather than crumbles, Cheese, yellow, American, pasteur-
cracks ized process deli‡, 1/2 in. slice
(5.0)
Dried raisin; sun-dried seedless, 1tsp
(10.0)
6 Rate of melt The degree to which sample melted Cream cheese; Kraft/Philadelphia‡,
in the mouth 1/2 in. cube (6.0)
Condensed milk; sweetened, Nestle
Original, 1 tsp (13.0)
7† Tooth packing The degree to which product sticks Carrot, fresh cut, 1/2 in. slice (1.0)
on the surface of teeth. Potato chips; Lay’s Classic‡ (9.8)
Taste
1§ Salty The taste induced by NaCl. NaCl solution 1.0% (18.0)#
NaCl solution 1.4% (22.0)#
NaCl solution 2.0% (27.0)#
2 Umami The taste induced by MSG. MSG solution 0.2% (2.5)#
MSG solution 0.35% (5.0)#
MSG solution 0.5% (8.5)#
Flavor
1§ Soybean-like The taste associated with cooked Soymilk, Vitasoy San Sui Soymilk
soybean or soymilk with beany Low Sugar‡, dilute with distilled
flavor. drinking water, 1 mL/9 mL (2.0).
Soymilk, Vitasoy San Sui Soymilk
Low Sugar‡ dilute with distilled
drinking water, 4 mL/6 mL (5.0).
Soymilk, Vitasoy San Sui Soymilk
Low Sugar‡ (13.0).
2§ Sesame oil-like The flavor associated with oily, Sesame oil; Lee Kum Kee‡, blended
nutty, buttery or other characteris- with plain tofu; Pak Fuk Beancurd
tics of sesame oil for steaming‡, 1 mL/30 g (2.0).
Sesame oil; Lee Kum Kee‡, blended
with plain tofu; Pak Fuk Beancurd
for steaming‡, 3 mL/10 g (7.0).
Pure sesame oil; Lee Kum Kee‡
(13.0).
TABLE 3. CONTINUED
Notes:
* Intensity is based on a 15-point numerical line scale with 0.5 increments, where 0 is “none” and 15 is “extremely strong.”
† References were initially prepared following that of Meilgaard et al. (2006) followed subsequently by consensus from panel discussion.
‡ Bold characters indicate the brands/species of food used in this study.
§ References were first prepared based on that of Fung (2004) with subsequent modification through panel discussion and consensus.
# Percentage (%) is mathematically [solute (mass, g)/water (volume, mL)]3100%; e.g., 1.0% NaCl solution is prepared by 10.0 g NaCl in 1 L
distilled water.
k The blended fish-water mixture was filtered through sieve (Model: S.T. 18-8, Sieve diameter 15 cm, pore size: 1 mm2, Japan), to prepare the
corresponding reference solution.
Statistical Analysis list in Table 1, including their origin, ingredients, and mois-
ture content. The ingredients of the products were similar
Differences between sample means were considered signifi-
including soybean, rice wine, salt, and water, and the mois-
cant at P < 0.1 or P < 0.05, or both, in one-way analysis of
ture content of all samples was high (Table 1). The PCA
variance (ANOVA) followed by the Duncan test, performed
results (Fig. 1) placed moisture content in the third quad-
using SPSS 17.0 (SPSS, Chicago, IL). Principal component
rant, opposite to the attributes cohesiveness (texture) and
analysis (PCA) was used for analyzing the data to study their
sesame oil-like (flavor); this suggests that moisture content
patterns. PCA was performed and figures were drawn using
might be low in samples whose cohesiveness and sesame oil-
R 2.8.0 with the software package SensoMineR v.108.
like intensities are high. This result was obtained for C-12:
the product featured the lowest water content (177%) and
was ranked the highest in terms of the intensity of cohesive-
RESULTS AND DISCUSSION ness and sesame oil-like (Table 5). In Fig. 2, the samples are
sorted according to their manufacturing locations. The
Basic Characteristics of Commercial Plain
moisture content was highest in the samples produced in LA
Sufus
(A-1–A-6), >250% on a dry-weight basis, and this was fol-
Basic information of 12 brands of plain sufu, which were lowed by the moisture content in LB samples, 220–250%,
manufactured in locations A (LA), B (LB), or C (LC) were with the exception of B-10 (265%). The water content was
Notes: Intensity is based on a 15-point numerical line scale with 0.5 increments, where 0 is “none” and 15 is “extremely strong.” Each value is
mean from n521 responses, i.e., 7 panelists with triplicate evaluations. Significant difference of each attribute among different sufu brands was
determined at P50.1 level by one-way ANOVA. Different letters (a, b, c, etc.) signify different levels between sufu brands.
was confirmed to be a key compound that presents a meaty soy sauce. The aroma “fish-like” is associated with fish or
odor (Chung et al. 2005). shellfish or both, such as dried fish and clams.
The results of sensory tests showed that the estimated The tested samples showed no significant differences in
intensities (<5.0) of both “moldy” and “meaty” were typi- the attributes “soybean-like,” “sesame oil-like,” and
cally less than those of all other attributes among the samples “fermented” (P > 0.05) (Table 4). Soybean and sesame oil
(Table 4), except in a few cases. It could be observed that the are both major ingredients used for producing sufu, and
“moldy” intensity of B-7 was significantly higher than that thus the attributes “soybean-like” and “sesame oil-like”
of both B-11 and C-12, and the “meaty” intensities of B-7 might be closely related to the presence of these ingredients.
and B-10 were statistically higher than that of the remaining “Fermented” is a common descriptor used to describe fer-
samples, which might be due to the variations in their mented foods such as soy sauce (Cherdchu et al. 2013), kim-
methional concentration. The concentration of methional chi (Chambers et al. 2012), and dairy protein hydrolysates
might also be related to the maturity of the products, (Newman et al. 2014). Moreover, “fermented” is likely
because methional is detected at the end of fermentation related to sufu maturity; volatile nitrogenous compounds,
(Chung et al. 2005). The intensity of “salty” was high and such as 3-methylbutanamide, that are degradation products
covered a wide range (4.5–8.5) (Table 4); “salty” refers to the from protein hydrolysis contributing to its final flavor
salty food aroma that emanates from products that contain (Chung et al. 2005; Yasuda 2011) and might be responsible
high concentrations of sodium chloride, such as fermented for endowing the attribute “fermented.” The intensities of
Notes: Intensity is based on a 15-point numerical line scale with 0.5 increments, where 0 is “none” and 15 is “extremely strong.” Each value is
mean from n521 responses, i.e., 7 panelists with triplicate evaluations. Significant difference of each attribute among different sufu brands was
determined at P50.1 level by one-way ANOVA. Different letters (a, b, c, etc.) signify different levels between sufu brands.
TABLE 6. INTENSITY SCORES FOR TASTE AND FLAVOR ATTRIBUTES OF 12 BRANDS OF PLAIN SUFU
Brands Salty Umami Soybean-like Sesame oil-like Fermented Fish-like Alcohol-like
a a a a a a
A-1 12.6 6 1.5 3.7 6 1.3 7.3 6 1.8 1.2 6 1.1 2.6 6 1.7 2.3 6 1.5 3.2 6 1.5a
A-2 12.3 6 1.8a 5.0 6 1.6a 7.2 6 2.0a 1.6 6 1.2a 2.7 6 1.5a 2.7 6 1.8a 2.4 6 1.7a
A-3 12.4 6 1.5a 3.8 6 1.7a 8.3 6 1.6a 1.8 6 1.8a 2.5 6 1.5a 2.9 6 1.8a 2.1 6 1.4a
A-4 12.4 6 1.8a 3.6 6 1.7a 7.6 6 1.6a 2.1 6 1.7a 2.4 6 1.1a 2.4 6 1.6a 2.8 6 1.5a
A-5 12.2 6 1.5a 5.7 6 1.9a 7.8 6 1.2a 1.4 6 1.2a 2.0 6 1.5a 1.8 6 1.3a 1.8 6 1.3a
A-6 12.0 6 1.7a 5.1 6 1.9a 7.5 6 1.7a 1.5 6 1.3a 2.1 6 1.4a 2.2 6 1.6a 2.2 6 0.9a
B-7 12.7 6 1.5a 5.3 6 1.7a 6.3 6 1.9a 2.2 6 1.6a 3.1 6 1.8a 2.1 6 1.3a 1.9 6 1.5a
B-8 12.2 6 1.7a 4.3 6 1.9a 6.4 6 1.6a 2.4 6 1.7a 2.5 6 1.5a 2.5 6 1.8a 2.0 6 1.4a
B-9 12.4 6 1.7a 4.6 6 1.8a 7.3 6 1.8a 2.5 6 1.6a 2.8 6 1.7a 2.8 6 1.6a 2.0 6 1.5a
B-10 12.6 6 1.6a 5.1 6 1.8a 7.6 6 1.3a 1.8 6 1.4a 2.4 6 1.8a 2.3 6 2.0a 2.2 6 1.1a
B-11 12.6 6 1.3a 5.7 6 1.5a 7.0 6 1.1a 1.9 6 1.7a 2.8 6 1.7a 2.9 6 1.4a 2.0 6 1.6a
C-12 11.8 6 1.3a 5.5 6 1.9a 7.1 6 1.6a 4.6 6 1.3b 2.3 6 1.7a 3.4 6 1.8a 1.1 6 0.8a
Notes: Intensity is based on a 15-point numerical line scale with 0.5 increments, where 0 is “none” and 15 is “extremely strong.” Each value is
mean from n521 responses, i.e., 7 panelists with triplicate evaluations. Significant difference of each attribute among different sufu brands was
determined at P50.1 level by one-way ANOVA. Different letters (a, b, c, etc.) signify different levels between sufu brands.
“alcohol-like” were moderate and in a middle range (2.5– and casein protein hydrolysates (Newman et al. 2014). Com-
6.5) (Table 4). An omission test confirmed that the volatile pared with other amino acids, both aspartic acid and glu-
compound 2-methoxyphenol is an essential component of tamic acid have been reported to be present at the highest
plain sufu (Chung et al. 2005), and this compound contrib- level in sufus (Li et al. 2010), and these amino acids were
utes to the alcohol-like attribute of samples. However, reported to not only enhance the umami taste and provide a
“alcohol-like” might also be related to the rice wine used in pleasant sensation, but also act synergistically with sodium
producing samples. chloride (Lioe et al. 2010). “Umami” was suggested to be an
Cohesiveness was rated from 2.3 to 4.8, and it was the irreplaceable taste in fermented food. “Sesame oil-like” was
only texture attribute that differed (P < 0.1) among samples one of the key flavor attributes identified here, and its inten-
(Table 5). The texture of the original tofu used for producing sity ranged from 1.2 to 4.6 (Table 6). Umami intensity was
the sample C-12 likely differed from that used in producing high in C-12, at least twice as high as that in all other sam-
other samples. Previously, the glycinin structure was shown ples, and this might be because of variations in the produc-
to undergo gradual and partial degradation, and the remain- tion methods used by distinct manufacturers; in C-12, the
ing a0 subunit of glycinin and the generated polypeptides content of added sesame oil might have been higher than
(MW: 30–32 and 10–15 kDa) detected after ripening were that in other samples.
reported to exhibit strong gelling ability (Mo et al. 2011;
Yasuda 2011). In terms of texture, as compared with fresh Similarities and Differences among
tofu (Yasuda 2011), plain sufu was smoother (roughness: Commercial Plain Sufus
1.4–3.1), softer (hardness: 6.5–8.5), and melted more readily To clearly illustrate the grouping among the plain sufus of
in the mouth (rate of melt: 7.9–10.0) (Table 5). distinct brands, PCA was performed on the intensity of the
Umami and saltiness are the 2 basic tastes detected in sufu 22 attributes collected from the trained panelists (Table 7).
products. Salty taste is contributed by the sodium chloride The factor loadings, also known as component loadings in
added to raw materials during production. Saltiness was PCA, are the correlation coefficients between the variables
extremely intense in the tested products, and the original ref- (rows) and the factors (columns). Analogous to Pearson’s r,
erence scale for saltiness (Meilgaard et al. 2006) did not cover the squared factor loading is the variance in the percentage
the level of saltiness detected in samples; thus, the original of a variable that can be explained by the factor (Husson
15-point scale was expanded, as shown in Table 3. NaCl sol- et al. 2010). As shown in Table 7, the majority of the attrib-
utions at 1.0%, 1.4%, and 2.0% were used as the references utes could be explained by the first 4 principal components
for “salty” intensities of 18.0, 22.0, and 27.0, respectively. (PCs), with a total covered variance of >75.0%. Most of the
Umami, the fifth of the 5 basic tastes, is mainly contributed attributes featuring high correlation coefficients on PC1
by free amino acids and peptides partially hydrolyzed from belonged to either aroma or flavor attributes, whereas on
soybean protein (Yasuda 2011). Free amino acids are key PC2, they belonged to the texture attribute.
flavor-enhancing compounds in numerous fermented foods Additional PCA was performed on only the 6 attributes
(Lioe et al. 2010). The umami taste was reported to be that showed statistically significant differences, and these
intense in commercial Doenjang products (Kim et al. 2010) could be explained by the first 2 dimensions (>81% of the
TABLE 7. PRINCIPAL COMPONENT ANALYSIS OF PLAIN SUFU BEFORE NOISE WAS ELIMINATED
Principal Explained Sensory attributes (correlation coefficient with absolute value > |60.50|)
Component (PC) Variability Aroma Texture Taste and flavor
Notes: Numbers in bracket are correlation coefficient in the range of 21.0 to 11.0. Sensory attribute with positive or negative correlation coeffi-
cient corresponds to positive or negative correlation, respectively. Attributes with absolute value < |60.50| are not shown. Attributes with correla-
tion coefficient either >0.5 or <20.5 (i.e., absolute value > |60.50|) are considered important in that dimension, which means the dimension
could be explained by those attributes.
variance) (Table 8; Fig. 1). PC1 explained 54.24% of the var- required to confirm this hypothesis. The salty (aroma)
iance contributed by the aroma attributes [soybean-like intensity was lowest in B-7 and B-10 and highest in C-12
(0.86), moldy (20.72), and meaty (20.52)] and the texture (Table 4), and the sesame oil-like (flavor) intensity in C-12
attribute [cohesiveness (0.80)]. PC2 explained 27.61% of the was considerably higher than that in all other products
variance, and the flavor attributes loaded on PC2 included (Table 6). The attributes “meaty” (aroma) and
alcohol-like (20.65) and sesame oil-like (0.86) (Table 8). “cohesiveness” (texture) differed moderately among the
Figure 1 shows the PCA results obtained using various products (P < 0.1) (Meilgaard et al. 2006) (Fig. 1). The
sensory attributes, with moisture content serving as a sup- meaty (aroma) intensity was lowest in A-2 and highest in
plementary variable. The attributes “moldy” (aroma), B-7, B-8, and B-10 (Table 4). Lastly, the intensity of cohe-
“alcohol-like” (aroma), “salty” (aroma), and “sesame oil- siveness was highest in C-12 and lowest in A-1 (Table 5).
like” (flavor) differed significantly among the tested prod- Cohesiveness was also identified as one of the main attrib-
ucts (P < 0.05) (Fig. 1). The moldy (aroma) intensity was utes in reduced-fat cheeses (Poltorak et al. 2015).
lowest in the products B-11 and C-12 and highest in B-7. Examination of the relationships between the origin of the
In previous study, moldy was identified as a key attribute plain sufus and their attributes revealed that products of a
in soy sauce and showed strong internal correlation with similar origin tended to show similar sensory profiles,
other flavors (Cherdchu et al. 2013). A-1 had the highest whereas other products did not (Fig. 2). The variation in the
alcohol-like (aroma) intensity, following by A-2 and A-4. moisture content of the products (Table 1; Fig. 1) indicated
Because alcoholic compounds might be generated during that this feature could serve as a potential maker for separat-
the post-fermentation stage, we expect the alcohol content ing samples originating from distinct locations. The results
in mature samples to be higher than that in freshly pre- show that all products prepared in LA, except A-4, were dis-
pared samples; however, additional experiments are tributed mainly in the negative part and were either above
TABLE 8. PRINCIPAL COMPONENT ANALYSIS OF PLAIN SUFU AFTER NOISE WAS ELIMINATED
Sensory attributes (correlation coefficient with absolute value > |60.50|)
Principal Component (PC) Explained Variability Aroma Texture Taste and flavor
PC1 54.24% Salty (0.86) Cohesiveness (0.80)
Moldy (20.72)
Meaty (20.52)
PC2 27.61% Alcohol-like (20.65) Sesame oil-like (0.86)
Notes: Numbers in bracket are correlation coefficient in the range of 21.0 to 11.0. Sensory attribute with positive or negative correlation coeffi-
cient correspond to positive or negative correlation, respectively. Attributes with absolute value < |60.50| are not shown. Attributes with correla-
tion coefficient either >0.5 or <20.5 (i.e., absolute value > |60.50|) are considered important in that dimension, which means that dimension
could be explained by those attributes.
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