KIDNEY- BOBA

LAB MIKROBIOLOGI

1. Peptone- water sugar reactions

- A series of peptone- water sugars
Glucose—mannitol—lactose—sucrose—dulcite—urea
- Can be used for the biochemical differentiation of E. coli
- Acid production red
- Gas positive bubbles

Proteus spp.

Glucose +

Urea +

Gas -

E. coli
Glucose +, Gas +
Mannitol +, Gas +
Lactose +, Gas +

2. Voges-prokauer (VP) test

Some Enterobacteriaceae ferment glucose with the production of acethyl methylcarbunol
oxidized and reacts with α-naphthol red color
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E. coli VP negative

3. Indole tube test

Indole positive bacteria break down amino acid trypthopan to indole reacts to Kova’s
reagent red color

E. coli indole positive

4. Nitrate reduction test

Organism is incubated in a broth containing nitrate and being observed after 4h red color (+)
E. coli positive nitrate production

5. API
These kits use dried reagent in cupules to which a suspension of the test organisms is added.
The strips are incubated overnight and the reactions determined. This allows the rapid
processing of a large number of isolates.
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PROTEUS SPP—ONPG- GLU- ARA- LDC- ODC-CIT-H2S-URE-TDA-IND

6. Methyl Red Test

E. coli is one of the bacteria that

produce acids, causing the pH to drop
below 4.4. when the pH indicator
methyl red is added cherry red
(positive MR test)
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7. Citrate test

Simmon’s media contains bromthymol blue, a

pH indicator yellow at acidic (around 6) and
gradually changes to blue at alkaline pH
(around 7,6)
E. coli negative citrate

8. Candida spp.
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Candida albicans hijau

Candida parapsilosis biru

CHROMagar Candida for isolation and

differentiation of major clinical significant
Candida species

Typical ppeareance:

Candida albicans green

Candida tropicalis metallic vlue

Candida krusei pink, fuzzy

Other species white to mauve

Candida dublinensis
Candida krusei
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9. URINE CULTURE
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10. Proteus spp

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bacteria urine culture

11. Staphylococcus aureus

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Culture on MSA (mannitol salt agar), showing yellow colonies. This is a selective medium for
recovering S. aureus when screening for carriage in infection control investigations.

12. Staphylococcus saprophyticus

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13. Pseudomonas aeruginosa

Culture on nutrient agar, produce a greenish

pyocyanin pigment, non-lactose fermenter.
It is oxidative positive.

A gram stain of patient’s specimen showing thin, gram negative bacilli

14. E. coli
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Microscopic: gram – bacilli, typical enterobacteria most have a similar morphology and can’t be
distinguish by gram staining
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15. Staphylococcus sp
Gram + cocci in clusters