Work Log

Date Job and Role What I accomplished

02/09/2019 Research some information t I found that polyphenols are t

o create a topic to research. he interesting chemicals that h
ave in fern and it can protect t
he skin from UV rays by inges
tion.

04/09/2019 Research more about the typ
es of polyphenols and some
kinds of eatable foods that c
ontain polyphenols and they
are abundant in Thailand.
For the edible foods that conta
in polyphenols are chocolate,
green tea and grapes but they
are not that abundant in Thaila
nd.

On the other hand, flavonoids

are one of the types of polyph
enols that can be found easily
in fruits and vegetables. Flavo
noids have a lot more of benef
it since it is the specific type o
f polyphenols.

06/09/2019 Research about edible flowe I found that Chrysanthemum a

rs that contain flavonoids an nd Roselle flowers are abunda
d the process of extraction. nt in Thailand and both of the
m is edible.

For the process of extraction, I

’m going to extract flavonoids
by the process of distillation h
aving ethanol as the solvent si
nce it can evaporate easily and
it is polar and nonpolar in its p
roperty.

11/09/2019 Research about how to find t I found that the presence of fla
he presence and the concentr vonoids can be indicated in m
ation of the flavonoids. any ways such as dropping hy
drochloric acid or ammonia.

To find for the concentration

of the flavonoids, I found that
it can be found by using the tit
ration.

12/09/2019 Research for the concentrati The concentration of ethanol t

on and boiling of the ethanol hat I’m going to use is a 60%
 and also the boiling point of ethanol and its boiling point is
the flavonoids. around 70-80 degree Celcius.

The boiling point of flavonoid

s is between 134-178 degree
Celcius.

23/09/2019 Research more about extract I found that it is better to extra

ing flavonoids to be sure bef ct flavonoids with rotary evap
ore going to the lab. orator since it can get more co
ncentrated distillate.

After the extraction, the flavo

noids must be kept in the refri
gerator.

Moreover, I can find the conc

entration of flavonoids by co
mparing with its standard wav
elength by using the spectroph
otometer.

25/09/2019 Research for the standard wa I found that the average stand
velength of the flavonoids. ard wavelength of the flavonoi
ds in chrysanthemum is appro
ximately 418.5 nm while rosel
le’s wavelength has around 51
0 nm since it contains anthocy
anins which are another type o
f flavonoids.

26/09/2019 Research for the quercetin e After I had to search for the e
quation. quation, I felt confused with t
he procedure since there are lo
ts of equations on the internet
and I didn’t know which one s
hould I use for my experiment
so I went ask my mentor and
my mentor said that we were
going to find another way to p
rove that chrysanthemum and
roselle can protect the UV rad
iation since this method is quit
e hard for me to do. So at last,
I found that I’m going to use l
ux meter to measure for the li
ght intensity and use the UV i
ndicator to double-check whet
her the samples prevent the U
 V radiation or not.

8/10/2019 Going to the lab to experime I learned lots of things in the e

10/10/2019 nt. xperiment. I learned how to be
16/10/2019 more focus and also how to ap
17/10/2019 +calculation ply my knowledge to use in th
e lab. Moreover, I knew how t
o handle errors that were occu
rred in the lab, if I were to exp
eriment again.

I could conclude that my hypo

thesis was supported by my ex
periment according to my calc
ulation and the result of the ex
periment.

27/11/2019 Work on lab report. There are a lot of information

needed to be put in the lab rep
ort. These make the lab report
consist

13/01/2020 Do the second experiment. For my second experiment, I’

m going to find the presence o
f flavonoids by using several t
ests to indicate the presence of
the substance.

The result shows that my 2 sa

mples do contain the flavonoi
ds substance.

20/01/2020 Add more information on la As I had gone to the lab to do

b report. my second experiment, I have
to write and record the data an
d analyze the result of my sec
ond experiment.

07/03/2020 Start making slides for prese Since I’m done with my lab re
ntation. port. My next task is to make
slides and get ready for my pr
esentation.

09/03/2020 Done with making slides. I’m done with my draft slides
but I think I’m going to read t
hrough them and add more det
ails in again.

30/04/2020 Editing and add more details All of the important details ar
. e included.