QC-200-S-005-1701 E

AUTOCLAVE
(Steam Sterilizer)

CL- 325 132L

405 140M I 40L
Instruction Manual

• Make sure to read this manual to use this product safely .

• Place this manual in a convenient location for easy reference.

ALP Co.,Ltd.
Tokyo Japan
Contents
1. Preliminary notes P.3

2. For the safety use P.3 to 6

3. Part names P.7 to 8

4. Installation P.9 to 15

5. Operation method P.16 to 38

6. Safety device P.39

7. Trouble shooting P.40 to 43

8. Maintenance P.44 to 51

9. Specifications P.52

10. Disposal and dismantlement P.53

11. Appendix P.54 to 56

2
 1. Preliminary notes
• Thank you for choosing ALP products.
Keep this manual at your hand for your quick reference whenever operating this autoclave.

• Do not use this autoclave for improper purpose. Strict observance of this manual is required.

:. • Please do understand and observe safety instructions written in this manual.

Failure to follow safety instructions may cause trouble and I or death.
:.
• Following documents are attached to this autoclave. Please check and if some of them are
missing, contact local distributor.
• Instruction Manual
• Certificate of Quality

• The lid is not opened until putting the power on.

(Refer to 4. 4 Power cable connection and protective grounding)

2. For the safety use

Preamble
Autoclave is being operated with high temperature and high pressure. Wrong installation
and lor failure to observe safety operation according to the instruction manual may cause
death or injury of operator and I or person near the autoclave, and I or destruction of
things.
Please read this chapter carefully then operate this autoclave. Important statements are
illustrated as follows. Please understand each meaning and observe these cautions.

The meaning of each illustration is as follows;

.& WARNING :Failure to this warning may cause death or serious injury of person .

.& CAUTION .Fallure to this caution may cause injury and

things.
I or destruction of

(S) Prohibited
o Strictly Observed

3
 • How to use autoclave
&WARNING
o This autoclave sterilizes objects with high temperature and high pressure.
Do not operate putting following materials and I or dangerous substances
(Refer to 11.3 Dangerous substances list) in chamber .
• Explosive materials •Flammable materials • Acid or Alkaline materials
•Corrosive materials •Non heat-resistance materials
o In case of sterilization of saline objects such as seawater, discharge saline water after
sterilization. Then put fresh water and operate several times without load. If you spill saline
objects, be sure to wipe off.
Failure to do them may cause explosion due to corroded chamber and pipes.
o Do not disassemble and I or modify autoclave.
Autoclave being pressure vessel. disassemble and I or modification of autoclave may cause accident and I or
breakage.
o Do not move autoclave holding lid nor push it hard.
Lid may jolt out of alignment which leads to serious accident and I or trouble.
o Do not get autoclave wet.
If electric component get wet, it may be broken. get a shock. and even cause fire.

• Installation
&WARNING
o Do not use power strip. (Refer to 4. 4 Power cable connection and protective grounding)
If power cable is bundled or tangled and lor wrongly connected. these may cause fire and I or get a shock.
o Protective grounding conductor must be connected to protective grounding terminal.
Connection to gas pipe or water pipe may cause explosion. get a shock and lor breakage.
o Do not install autoclave in the place where chemicals such as flammable materials, explosive
materials, and lor corrosive gas are kept.
Installing autoclave in above mentioned place may cause serious accident and/or trouble of autoclave.

&CAUTION
o This autoclave should be used indoors. Do not install it outdoors.
There are many possibilities of malfunction and/or breakage of autoclave if it is used outdoors.
o This autoclave must be installed on level and hard floor.
Installation without stability may cause malfunction and lor trouble.

• Cleaning
&WARNING
o Do not use cleaning products for cleaning the inside of the chamber.
It may cause explosion due to corroded metallic parts.

&CAUTION
o Do not use liquid which corrode metal and/or paint (EX. thinner, hypochlorous acid, chlorine
dioxide, acetyl hydroperoxide) for cleaning the exterior.
Metallic parts may corrode.
o Do not splash chlorine chemical and/or cleaning products (EX. hypochlorous acid, chlorine
dioxide, acetyl hydroperoxide) to the exterior during cleaning the floor.
Metallic parts may corrode.

. -
• Notes on the operation

~WARNING
o Be sure to close lid to prevent pinching dusts before operation.
If there is something between lid and chamber which prevents lid from closing, the lid may burst at high pressure
which cause fatal accident or serious injury. Then operator may get burned by steam leaked from chamber.
o Be sure to slide lid lock lever slowly to close position (left end) before operation.
If lock is not enough, operator can not start operation (Er4 is shown). But if operator can start operation by
malfunction of detection part, lid may explode at high pressure which cause fatal accident or serious injury.
o Objects (including disposable plastic bag) must be put in stainless wire basket or stainless bucket
when sterilization.
If operator loads objects into chamber directly, it may cause choking pipes which may lead to serious accident.
<9 Do not open lid forcibly during operation.
Opening lid forcibly during operation may cause fatal accident or serious injury.
<9 Do not open lid until chamber pressure goes down to "OMPa" when operation completed or
operation interrupted.
Steam spouts out of chamber by residual pressure which may cause burn injury.
o In case of sterilizing liquid objects, cool down objects enough after sterilization. Then unload
objects slowly.
liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber. Therefore extreme temperature change when unloading may cause high temperature liquid
spouting. It may cause burn injury.
o In case of sterilizing liquid objects, metallic or heat-resistance glass container must be used.
A glass container without heat-resistance may be easy to break by extreme temperature change and operator may
be injured by this breakage.
<9 In case of sterilizing liquid objects (EX. liquid media), do not close container with lid or bung which
has no breathabllltv.
It may cause rise of pressure in container, because temperature in container cool down slowly. Especially glass
container is easy to explode if it is damaged.
<9 Do not touch autoclave more than necessary.
If operator touches autoclave more than necessary, it may cause serious accident.
<9 Do not open drain valve under following conditions.
- during operation
- chamber temperature is high
- chamber pressure is not OMPa
It may cause burn injury by blow-out of boiling water and / or steam, water lack, and/or malfunction.
<9 Do not touch power switch(breaker) by wet hand.
Operator may get electric shock.

~CAUTION
o Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove, operator may get burned.
<9 Do not touch exhaust bottle and exhaust hose during air evacuation and exhaust.
It may cause burn injury. Do not touch them until cooling down enough.
<9 Do not touch stainless lid directly when opening or closing lid.
It may cause burn injury. Do not touch it until cooling down enough.
o Genuine accessories and spare parts made by ALP Co., Ltd. must be used.
Using other company's products may cause accident and lor breakage of autoclave.

5
• Notes on power failure

In case of power failure during operation, autoclave works as follows.

(Refer to 3. Part names Operation panel about LAMP, KEY, and DISPLAY)

1. Power is cut and stop working.

2. When power is supplied again, PROCESS INDICATION BAR lights up to show the status
when power failure happened. Also, TEMPERATURE DISPLA Y shows current chamber
temperature. In case of sterilization, TIMER DISPLAY shows remaining time of
sterilization, too.
3. Cool down the inside of the chamber enough.
(Press EXHAUST KEY to shorten cooling time if needed.)
4. When TEMPERATURE DISPLAY indicates BO°C, COMPLETE LAMP blinks.
5. Press STOP KEY to return to ready condition, and COMPLETE LAMP lights off.
6. After checking if pressure gauge indicates OMPa, slide the lid lock lever slowly to open
position (right end) with pressing front part of the lid cover. Then, open lid.
7. If sterilization is incomplete, set the condition of operation again then sterilize objects.
(Refer to 5. Operation method)

~WARNING
('9 Do not open lid until pressure gauge indicates OMPa and TEMPERATURE DISPLAY indicates below
80°C when operation interrupted.
Steam spouts out of chamber by residual pressure which may cause burn injury.
('9 Do not open drain valve under following conditions.
- chamber temperature is high
- chamber pressure is not OMPa
It may cause burn injury by blow-out of boiling water and / or steam. water lack. and/or malfunction .
•• Be sure to set automatic exhaust OFF when sterilizing liquid object or glass container.
(Do not press EXHAUST KEY.)
Quick reduction of chamber pressure may cause breakage of glass container or blow-out of hot liquid .
•• When sterilizing liquid, it takes longer to cool. When opening the lid, pay attention not to get burned
then unload the objects avoiding the impact to them.
liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber.

~CAUTION
•• Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove. operator may get burned .
•• Manual exhaust under 121°C is desirable.
Exhausted steam from the chamber is not cool enough which may cause burn injury.
And water in exhaust cooling tank reduces faster therefore frequent water supply is required.

6
 3. Part names
Main body
Lid cover
Lid lock lever

Operation panel
:.

Pressure gauge Lid hold

(both side)

Power switch
(Breaker)

Drain outlet

Exhaust bottle

Front caster with stopper

Exhaust cooling tank

(inside of the exterior)

Power cable

7
 /
1. Pressure gauge 14. STERILIZE LAMP
It indicates chamber pressure. The character lights up during sterilizing process. The
2. TEMPERATURE DISPLA Y character blinks at sterilizing setting change
It displays temperature in the chamber or setting condition.
temperature. Also. it displays the date of an operation 15. EXHAUST LAMP
reservation. The character blinks during exhaust process.
3. TIMER DISPLA Y The character lights up before exhaust process in
It displays the remaining time to the process case of automatic exhaust ON.
completion. "down count" Also. it displays the time of 16. WARM LAMP
an operation reservation and error. The character lights up during warming process. The
4. START KEY character blinks at warming setting change
Press it to start operation. condition.
5. STOP KEY 17. COMPLETE LAMP
Press it to stop operation on the way. Press it to reset The character lights up with the buzzer beeping at
previous operation before starting operation. the completion of all the processes. The character
6. PATTERN/ENTER KEY blinks and reports whether lid can be opened when
Press it to fix an operation pattern and each setting. pressing STOP KEY during operation.
7. TEMP/TIME KEY
Press it to check setting temperature. setting time PROCESS INDICATION BAR
and the operation pattern. Press it to change setting They light up, blink. light off according to the status of
display's blink. process.
8. CHANGE ..•• KEY 18. HEA TING-1 PROCESS LAMP
Press it to increase the figure of the setting display. It blinks from start temperature of the sterilization to
9. CHANGET KEY the end of the air evacuation.
Press it to decrease the figure of the setting display. It blinks from start temperature of the dissolving to
10. EXHAUST KEY the dissolving temperature.
Press it to switch automatic exhaust ON and OFF or 19. HEA TlNG-2 PROCESS LAMP
set an automatic exhaust starting temperature. Press It blinks from the end of the air evacuation to the
it to exhaust manually. sterilization temperature.
11. START TIMER KEY 20. STERILIZATION PROCESS LAMP
Press it to make a reservation of operation. Set an It blinks during sterilization.
operation starting time of month. day. hour. and 21. COOLlNG-1 PROCESS LAMP
minute. It blinks to 100"C after sterilization.
12. READY LAMP 22. COOLlNG-2 PROCESS LAMP
The character lights up at the reservation condition. It blinks from 99"C after sterilization to the
The character blinks at setting change condition. completion.
13. HEAT LAMP 23. WARMING PROCESS LAMP
The character lights up during heating or dissolving It blinks during warming.
process. The character blinks at dissolving setting 24. COOLlNG-3 PROCESS LAMP
change condition. It blinks from 99"C after warming to the completion.

8
 4. Installation
4. 1 Transportation

This autoclave has casters. So it can move horizontally.

~WARNING
CS> Do not pinch hands I feet when moving autoclave.
There is a risk of a fracture or bruise.

~CAUTION
The autoclave must be moved by two or more persons.
~ Do not hit and lor get shock to autoclave nor hold lid cover. (Fig. 1)
It may cause breakage or damage of autoclave.
o In case of lifting the autoclave, be sure to use a heavy machinery. Do not lift by humans.
It may cause a falling accident.
o In case of lifting the autoclave by a forklift, be sure to insert the fork under the bottom plate
avoiding the casters. (Fig.2)
o In case of lifting the autoclave by a crane, be sure to hang the bands to four corners of the
bottom plate. Put cushioning between bands and the autoclave. (Fig.S)

Do not hold lid cover.

/
I I

Fig.1 Fig.2 Fig.3

9
4. 2 Installation location
Autoclave must be installed as follows.
Refer to 4.3-4.5.

Up to ceiling or duct
Wall
~ surface
-L00-L-L-L-L-L-L-L..,L~~-L-L-L-L-L.L....L....L..- Wall Pay attention to electric
surface components and alarms
150mm or more of the ceiling. 800mm or more

I
100mm or more
Wall surface
or other O:=iiIHIiiiHIiIHiiiIHiIiiiiiiIii=:O
0:::::::::::::::::::::::::::-
~
instrument 150mm or more

r----, Exhaust
Pour water to exhaust bottle
up to LOW level. and insert
r:
I
cooling
tank
exhaust hose into the bottle. I
I
Then. insert the bottle into I
main body. Do not bend the I
I
hose when insertin the bottle. I
I
I
I
I

Lock front casters L_

~ Level and hard floor

///////////////

10
 ~WARNING
(9 Do not install autoclave in the place where chemicals such as flammable materials, explosive
materials, and lor corrosive gas are kept.
Installing autoclave in above mentioned place may cause serious accident and/or trouble of autoclave.
o This autoclave must be installed to a place where operator can read pressure gauge, or it may
cause serious trouble.
(9 Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.

~CAUTION
o This autoclave must be installed on level and hard place.
o Keep 100 to 150 mm space between autoclave and wall or other instruments.
o Install autoclave avoiding humid and dusty place and keep away from direct sunlight.
o Lock stoppers of front casters.
(9 Do not install autoclave under electric components and alarms of the ceiling, or it may cause
malfunction of them by steam.
o Be sure to use exhaust bottle, or steam from chamber is splattered to outside which may cause
burning.
o Pour water to exhaust bottle up to LOW level.
If water level is under LOW level, steam spouts out directly which may cause burning.
(9 Autoclave must not be installed outdoors.
Breakage of autoclave may happen if it is installed outdoors.
(9 Do not use exhaust bottle for drain.
It may cause burn injury by blow-off of boiling water.

4. 3 Storage I Usage condition

Autoclave must be stored I used under following condition .

• Ambient temperature: 5 to 35°C • Atmospheric pressure 90 to 106kPa
• Relative humidity 30 to 85%
Do not store I use autoclave at locations as follows
·Outdoors
• Locations where receive direct sunlight
• Locations where autoclave get wet
• Dusty locations
• Sloping ground
·Locations with saline and lor sulfur in the air
• Locations except ambient temperature, relative humidity, and atmospheric pressure
• Locations where withstand load is lower than 150kg.

11
4. 4 Power cable connection and protective grounding

Lid is not opened until putting the power on. Slide lid lock lever to open position (right end)
after power on. Then open lid and unload all contents inside chamber.
(Refer to 4.5 Standard accessories)
Do not press START KEY during this operation. Otherwise contents inside chamber may
burn out due to the water lack.

Please note following points when power connection .

• Be sure to check voltage and allowable current value of outlet. Failure to connect outlet
with proper voltage and allowable current value may cause breakage.
(Refer to 9. Specifications)
• Be sure to connect the power cable to the breaker which meets below specifications.
CL-32S : AC220-240V, single phase, more than 15A
CL-32L : AC220-240V, single phase, more than 15A
CL-40S : AC220-240V, single phase, more than 20A
CL-40M : AC220-240V, single phase, more than 20A
CL-40L : AC220-240V, single phase, more than 20A
• Do not use power strip .
• When you connect power cable to distribution board directly, beware of faulty wiring and
electric shock.
•Grounding cable of the power cable must be connected to ground terminal of distribution
board.

&WARNING
o Be sure to connect the power cable to the breaker which meets specifications of the autoclave.
Grounding cable must be connected to grounding terminal.
~ Do not bend and / or bundle power cable.
Improper wiring may cause fire or power leakage due to bad electrical failure or malfunction.
o Do not put heavy things on power cable.
Improper wiring may cause fire or power leakage due to bad electrical failure or malfunction.
o Do not connect grounding cable to gas pipe, water pipe, telephone line or lighting rod.
Improper connection may cause fire or power leakage due to bad electrical failure or malfunction.
If connector with grounding terminal is not provided. make the connector with grounding terminal.
o Do not touch power connector by wet hand.
Operator may get electric shock.

~CAUTION
o Power cable must be singly-connected to the commercial power with proper voltage and
amperage.
Improper connection may cause malfunction or trouble.

12
4. 5 Standard accessories
CDRead the cautions of sticker which covers power switch(breaker), then remove it.
® Turn on power switch, then slide lid lock lever to open position (right end) and open lid.
@ Unload all standard accessories as below.
@ Set every accessory and pour water into exhaust water cooling tank following below
instructions.

o 0 0 0 0

,
Base plate, 1pc.
Exhaust bottle(3L), 1pc.

Base plate hook, 1pc.

Stainless wire basket Chamber drain tray, 1pc. (CL -40L only)
CL-32S: ¢ 300 x 200mm 2pcs.
CL-32L: ¢ 300 x 300mm 2pcs.
CL-40S: ¢ 380 x 200mm 2pcs.
CL-40M: ¢ 380 x 300mm 2pcs.
CL-40L: ¢ 380 x 400mm 2pcs .

• Base plate- •• Set it on the bottom of chamber.

Purpose of use: To protect objects from direct heat generated by heater.
Level of base plate is guide for proper water level.

.Exhaust bottle·· ·Set as the figure of "4. 2 Installation location".

Purpose of use: Receive steam and air in the chamber.

.Stainless wire basket· •• Use it during operation.

Purpose of use: Load objects into it for operation .

• Chamber drain tray··· Use it when draining water in the chamber.

Purpose of use: Receive drain water in the chamber.

13
.Base plate hook (for CL-40L only)- •• Keep it with this instruction manual.
Purpose of use: Tool for unloading base plate from bottom of chamber at the time of
maintenance.

OPouring water into exhaust cooling tank

Mixture of steam and air in chamber is evacuated during operation.
They get cold through exhaust cooling tank, then they are evacuated to exhaust bottle.
Pour water into the tank referring to following procedure.
(Tap water is available.)

CDDetach the right side panel using phillips-head screw driver.

® Pull out the plug from the funnel as figure 1.
@ Keep silicon hose straight and pour water into the tank as figure 2 on the next page.
Ideal water supply quantity: About 10L or water level of figure 3
@ Stopple the funnel.
@ Attach the right side panel using phillips-head screw driver.

Pouring water into the tank is not required in every operation.

However when steam is exhausted from exhaust bottle and/or water lack in the tank is
found by yearly maintenance (Refer to 8.3 Yearly maintenance), pour water following
above procedure.

II \V

E/I\
E
0
~
Funnel 0
.•....•
0
C'?

Plug

Figure 1 Figure 3

14
 Keep the silicon
hose straight.

Ideal water supply quantity:

About 10L or 90% of tank capacity

Figure 2

~WARNING
o Be sure to set base plate during operation.
Operation without base plate may cause burning of object which leads to fire.
o Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.

~CAUTION
e Do not pour too much water into exhaust cooling tank.
It makes overflow which may cause malfunction and/or breakage of autoclave.
o Water
Be sure to stopple the funnel after pouring water into exhaust cooling tank.
in exhaust cooling tank reduces faster therefore frequent water supply is required.
o Beburning.
sure to use exhaust bottle, or steam from chamber is splattered to outside which may cause

o Pour water to exhaust bottle up to LOW level.

If water level is under LOW level. steam spouts out directly which may cause burning.
o Do not use exhaust bottle for drain.
It may cause burn injury by blow-off of boiling water.

15
5. Operation method
&WARNING ----------------------,
o Be sure to read this chapter carefully and understand well before operation and use autoclave
properly, or it may cause fatal accident at worst as well as mechanical trouble .

• Flow chart of basic operation

1. Turn on power switch(breaker)

...J..L.
2. Close drain valve
.J L
3. Water supply
-L.L
4. Load object
-Ll-
5. Close and lock the lid
-Ll-
6. Press STOP KEY
I
J L
7. Set operation pattern,
value of temperature and time
-L.L
8. Press START KEY
-L.L
9. Operation complete
-L.L
10. Unload object
(below ao°c in chamber)
lL
11. Close and lock the lid
-L.L
12. Turn off power switch (breaker)

16
Before operation
~WARNING
o In case of sterilization of objects such as electrolyte-containing saline (EX. sea water), be sure to
discharge water in chamber and clean it after sterilization complete. Then put fresh water and
operate several times.
Failure to maintain after sterilization may cause corrosion of bottom of chamber by electrolytic corrosion which
leads to rot.
o Be sure to check if pressure gauge indicates "OMPa".
If pressure gauge does not work properly, please contact local distributor.
If pressure gauge is wrong. it may cause serious accident and/or breakage of autoclave.
o Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.
o Do not touch power switch(breaker) by wet hand.
Operator may get electric shock.

5. 1 Turn on power switch(breaker)

CD Check if power cable is connected properly.

® Power ON.

5. 2 Close drain valve

CD Unload exhaust bottle from housing hole, then close drain valve.(Check if it is closed.)

OPEN CLOSE
Drain valve
Inside of the ]
[ housing hole

~CAUTION
o Be sure to close drain valve.
If water leaks from chamber. it may cause water lack. malfunction and/or breakage of autoclave.

17
 5. 3 Water supply

5. 3. 1 Water supply

CD Check if pressure gauge indicates "OMPa".

® Open lid then pour water to the same level as base plate.
(CL-32S/32L : about 4L, CL-40S/ 40M/ 40L : about 6L)

Base plate Chamber

Proper level

Heater

&WARNING
\S) Do not open lid until chamber pressure goes down to "OMPa".
Steam spouts out of chamber by residual pressure which may cause burn injury.

&CAUTION
o Water level in camber is getting lower during operation. Check water level in every operation and
pour water to the same level if it's under proper level.
o Water put in chamber must be distilled one or purified one. Frequent cleaning of chamber is
requested when using city water which generates scale in it.
\S) Do not spill water on the autoclave when pouring it. If you spill water, wipe up it.
o Change water for sterilizing frequently. Water must be changed every day in case of daily use.
o Do not use well water and industrial water which may cause corrosion and/or smudge.
Failure to use proper water may decrease life of the heating element and/or the chamber.

18

r-- ~~ ~====~==~==~==~-==-_~=_~====~ ~~=__

5. 3. 2 Drain water from exhaust bottle
CDUnload exhaust bottle from housing hole and draw out exhaust hose from the
bottle.
® Pour water up to LOW level.
If the water level is over HIGH level, pour off the water down to LOW level.
@ Insert exhaust hose into the bottle.
The hose must not be folded when inserting. If folded, air cannot exhaust out from
the chamber and sterilization cannot be completed. Then, you cannot open the lid.
@ Insert the bottle into housing hole slowly.

&CAUTION
o Water level in exhaust bottle rises every operation. Keep the level between LOW and HIGH
at any time.
Operating over HIGH level is a cause of burn injury due to boiling over from exhaust bottle.
Operating under LOW level is also a cause of burn injury due to steam blowing.
OAt the time of drainage from exhaust bottle, check the bottle if it is cold enough, or it may cause
burn injury.
Exhaust bottle keeps high temperature soon after air evacuation and exhaust.

5. 4 Load object

CD Objects must be put in the stainless wire basket or the stainless bucket (optional
accessory), then set in the chamber slowly.

* In case of CL-40L, use a step as below figure when loading the stainless wire
basket(optional accessory) or the stainless bucket (optional accessory) into the
chamber.

fJJ
~------n!.\.-1)
._-------'

19
*Do not put too much objects in the chamber.
Too much objects in the chamber may cause sterilization incomplete.
(Quantity of objects to be put inside the chamber shall be less than 60% of the capacity
of chamber)

~WARNING
o Be sure to use the stainless wire basket, the stainless wire basket with pan or the stainless
bucket.
In case of sterilization without above accessories. the chamber pressure become out of control
due to the objects choke up the pipe which leads to serious accident such as explosion.
o Be sure that objects do not make contact with the inside of the lid.
lor serious accident.
If the lid is not closed properly. it may cause serious injury by blowing steam

~CAUTION
o In case of sterilization of flagons such as vial container, screw cap bottle, conical flask, take out
cap or screw down it.
(Do not close mouth of flagons with impervious material such as silicon or rubber.)
o In case of sterilization of liquid objects, quantity of object to be put inside container shall be less
than 80% of capacity of container.
Too much object in container may cause bumping when objects are taken out of the chamber after operation
complete.

20
.About a disposable plastic bag

When using a disposable plastic bag or other kind of bags, please be strictly observe following
warnings/ cautions.

~WARNING
o Be sure to put the bag into Stainless bucket or Stainless wire basket. (Fig. 1)
If the bag is put into chamber directly, autoclave may cause serious explosion. When exhaust holes located in the
wall of chamber are chocked by the bag, safety devices may not activate.
Following safety devices may not work due to choking of exhaust holes in chamber.
- Over pressure detector (Error message is shown on the display and buzzer beeping)
- Safety Valve (Steam evacuates out of chamber)
& Do not put too much bags into chamber. (Fig. 2,Fig.3)
Only single pack of bag must be put in chamber. (Fig.1)
Putting too much bags into chamber may cause serious explosion. When exhaust holes located in the wall of
chamber are chocked by the bag, safety devices may not activate.
o Be sure to fold back the mouth of the bag inside of Stainless bucket or Stainless wire basket.
(Fig.4, Fig.5)
If the mouth of bag is folded back outside of Stainless bucket or Stainless wire basket, autoclave may cause
serious explosion. When exhaust holes located in the wall of chamber are chocked by the bag, safety devices may
not activate.
& The bag must not be caught between lid and mouth of chamber.
Operator may burn by spouting hot water and/or steam which may leads to explosion, because the bag bursts in
chamber.

x x

Fig.l Fig.2 Fig.3

21
 o x
Fig.4 Fig.5

&CAUTION
o Do not put too much object in the bag.
(Quantity of object shall be less than 60-70% of bag capacity.)
Putting too much objects into the bag may cause improper steam penetration which leads to activation of
safety devices in addition to the sterilization incomplete.
o The bag must have open mouth.
When closing the mouth of the bag, residual air may cause sterilization incomplete.
If the bag need to be tied with band for reason of protective from hazardous substance, be sure to secure space
for the steam going through the bag. Or safety devices may activate in addition to the sterilization incomplete.
o For sure sterilization, put 100mQ to 200mQ water into bag.
Steam is generated in the bag which leads to getting better sterilization.
o Be sure to put the bag into genuine stainless bucket or stainless wire basket with pan made by
ALP Co., Ltd. when you put objects with sharp edge such as broken petri dishes, test tubes,
needles into the bag directly.
Using stainless wire basket may cause spillage of waste water in the chamber and breakage of the autoclave.
o Be sure to check the water level of chamber before operation.
Insufficient water in chamber may cause melting bag and stick on the base plate by directly heated from
heating element located in bottom of chamber.
o Sterilization time / temperature shall be longer/higher as the Quantity of objects is increased.
Sterilization conditions may vary depending on the objects. Use the chemical indicator and so on to get suitable
sterilization condition for each object.

Stainless wire basket Band

or stainless bucket or
stainless wire basket I Chamber I (Do not tie too much
to have open mouth)
with pan

Water
(approx 200m!)

Proper water level

in chamber

Heating element
Fig.6 Fig.7
Recommended method If you need to close the mouth of the bag to protect
from hazardous substance etc., instruction by Fig.7 is
one of the solution.

22
 .How to use stainless bucket (optional parts)

When using a stainless bucket, please strictly observe following warnings/cautions.

~WARNING
o Load stainless bucket in the center of chamber.
If the space between the bucket and the chamber's wall is not enough. exhaust holes located in the wall of
:. chamber may choke. It leads to serious accident.
Following safety devices may not work due to choking of exhaust holes in chamber.
- Over pressure detector
(Error message is shown on the display with buzzer beeping and power to heater is cut off.)
- Safety Valve (Steam evacuates out of chamber even if over pressure detector doesn't work oroperly.)

~CAUTION
(9 Do not stack stainless buckets up in the chamber..
If the space between buckets is not enough. the sterilization may be incomplete as air convection in the chamber
is insufficient.
(9 Do not overload objects which are difficult to penetrate steam (linen, gauze, etc+-), or it may
cause sterilization incomplete.
o Put water (200m!) into stainless bucket, or it may cause sterilization incomplete.
The sterilization time shall be longer if you sterilize without water.
(9 Do not put too much objects in the chamber for sterilization.
Quantity of objects to be put in the chamber shall be less than 60% of the chamber capacity.
o Sterilization time / temperature shall be longer/higher compared with stainless wire basket, as
evacuating air's efficiency of sterilization bucket is not good.
Use the chemical indicators and so on to get suitable sterilization condition.

spaces
bucketStainless

Water
(about 200m!)
Chamber
Objects
Base plate

Heater

23
 5. 5 Close and lock the lid

CD Grip lid hold then close lid slowly.

® Slide the lid lock lever slowly to close position (left end) with pressing front part of the
lid cover. CD

CLOSE oe===<>- OPEN

~WARNING
o Be sure lid gasket has no dusts and crack.
Wipe off dusts on gasket with a soft cloth.
If you find crack on gasket. please contact local distributor.
If you leave crack one unchanged. it may cause burst at worst as well as leakage of steam.
o Be sure to slide lid lock lever slowly to close position (left end) before operation.
If lock is not enough. operator can not start operation (Er4 is shown). But if operator can start operation by
malfunction of detection part. lid may explode at high pressure which cause fatal accident or serious injury.
(9 Do not slide the lid lock lever by force.
Forcing the lever to move may damage to mechanical locking device. it may cause unexpected accident.

~CAUTION
o Soon after previous operation (in case that TEMPERATURE DISPLAY indicates 50-79°C after
opening lid). wait for a minute with closing lid. then slide the lid lock lever.
Reactive force arises when closing lid because the air which was caught by lid is swollen by heat of chamber.
Therefore. it's necessary to keep the lid closing for a minute to discharge swollen air in chamber.

5. 6 Press STOP KEY

CD Press STOP KEY to reset completion process of previous operation, in case you start
new operation continuously.

~CAUTION
o Be sure to press STOP KEY before setting and starting operation.
If completion process of previous operation is not reset. you can not set and start new operation.

24
 5. 7 Set operation pattern, value of temperature and time
5. 7. 1 Operation pattern setting

Initial condition
Steri I ization Press PATTERN/ENTER KEY.
pattern
.:

r-------
,-----
I
I
I -,
_--;-;::' ",.--C~' I
MON
8 I' I
DAY °c
EXHAUST
STERILIZE
-
~~
TIME ~~

I
I START II HEAT \c::=:J \\
TIMER I, D
'READY
-- .•.

Press CHANGE •.•. KEY

Ster i I izat ion/
Warming pattern D to change pattern.

I -, I I 8
EXHAUST _STERILIZE

_ ---::::'
",.--C~'
lION DAY
°c I'
1-' • -, 1-' START
TIMER
II
I, HEAT \\-,
D WARM
U·Cu 'READY
-- .•.

-.- - .... ,.---

Dissolving/
Warming pattern D :__ .JI

L l
I\

\ i10N
nn~8
, L'
§
DAy
c
c::=:J

~
I
-----1/
, START

I
TIMER HEAT \\-, WARM

READ{
D
-- .•.

• Sterilization pattern
This operation pattern is for sterilizing objects .

• Sterilization / Warming pattern

This operation pattern is for sterilizing and warming objects .

• Dissolving / Warming pattern

This operation pattern is for dissolving and warming agar medium or the like.

25
 5. 7. 2 Temperature / time setting

CJ)Setoperation temperature and time in the below setting range.

Setting range

Sterilization pattern
CL-32S/32L : Sterilization ternp.: 100°C to 140°C
Sterilization time: Ominute to 48hours
CL-40S/ 40M/ 40L : Sterilization temp.: 100°C to 137°C
Sterilization time: Ominute to 48hours

Sterilization/Warming pattern
CL-32S/32L : Sterilization temp.: 100°C to 140°C
Sterilization time: Ominute to 48hours
Warming ternp.: 40°C to 60°C
Warming time: Ominute to 48hours
CL-40S/ 40M/ 40L : Sterilization ternp.: 100°C to 13rC
Sterilization time: Ominute to 48hours
Warming ternp.: 40°C to 60°C
Warming time: Ominute to 48hours

Dissolving/Warming pattern(for CL-32S/32L/ 40S/ 40M/ 40L)

Dissolving temp.: 40°C to ggoC
Dissolving time: Ominute to 48hours
Warming temp.: 40°C to 60°C
Warming time: Ominute to 48hours

.Continuous run setting

If you want to operate continuous run (long run over 48hours), contact local distributor.

~CAUTION
o In case object is a large amount of liquid or object is put in container which is difficult to
evacuate air, temperature of object rises slowly. In this case, set sterilization temperature
higher or set sterilization time longer.
Use the chemical indicators and so on to check sterilization condition for object periodically.

26
 5. 7. 2 Temperature / time setting

(j)Set operation temperature and time in the below setting range.

Setting range

Sterilization pattern
CL-32S/32L : Sterilization temp.: 100°C to 140°C
Sterilization time: Ominute to 48hours
CL-40S/ 40M/ 40L : Sterilization ternp.: 100°C to 137°C
Sterilization time: Ominute to 48hours

Sterilization/Warming pattern
CL-32S/32L : Sterilization ternp.: 100°C to 140°C
Sterilization time: Ominute to 48hours
Warming ternp.: 40°C to 60°C
Warming time: Ominute to 48hours
CL-40S/ 40M/ 40L : Sterilization temp.: 100°C to 137°C
Sterilization time: Ominute to 48hours
Warming ternp.: 40°C to 60°C
Warming time: Ominute to 48hours

Dissolving/Warming pattern(for CL-32S/32L/ 40S/ 40M/ 40L)

Dissolving ternp.: 40°C to ggoC
Dissolving time: Ominute to 48hours
Warming temp.:40°C to 60°C
Warming time: Ominute to 48hours

.Continuous run setting

If you want to operate continuous run (long run over 48hours), contact local distributor.

~CAUTION
o In case object is a large amount of liquid or object is put in container which is difficult to
evacuate air, temperature of object rises slowly. In this case, set sterilization temperature
higher or set sterilization time longer.
Use the chemical indicators and so on to check sterilization condition for object periodically.

26
 When press ~ sh ift tocJ
Initial condition
(In case of Steri Iization / Warming pattern)
In case of other patterns, operate in the same way.
/ ¥r
When press( / ~ sh ift
,
,/
, //

/
to: ~
r

----I
1\

r-----~\
~
\
".,,,
LI· CLI
PATTERN

~
I
HR MIN
---1'

To the mode of
D temperature / timer setting.

I
I
I
I
I
I
I
I
I
I

L_,
I
----,

J
I
/

"
I ,
Ie
MON

LI·CLI
. ",
I
I
DAY'-
°c
8XHAUST

I
, \
"
~E

HEAT
/

~WARM \ PATTERN

~
HR MIN
"
!EAO~
/

.
(//"
,
."

/
/
/
' .....
/

Change steri Iization temperature

D by CHANGEA ••• KEY.

II
-,
e I 18
0c EXHAUST
ST~RllfzE
,iiIiiiiiiiii\
~~
MON DAY

I
L
1 ,

JI
In ,
LI· _,LI
HR
• _,
- ,- ,

MIN
START
TIMER

-,
HEAT

JEAO:(
/
~-\ WARM

.
"
,
..•
~

-:.
/
.iNTER

/
[SZJ~
Change steri Iization time
D by CHANGEA ••• KEY.

-
STERILI ZE

'-
L

J
I
,
I,
MON

IC·
<ru»
LILI
DAY

HR MIN

Change warming temperature

D by CHANGEA'" KEY.

----I
MON
t:
o I, I 8
LI
DAY
-
STERILI ZE

HEAT
______ JI

HR MIN
I "JEAO:(/

Change warming time

by CHANGEA'" KEY.
27
5. 7. 3 Exhaust setting

CD Set automatic exhaust on/oFF referring to following statements.

® In case of on, set automatic exhaust starting temperature .

• Automatic exhaust

Automatic exhaust enables to exhaust steam to outside automatically after sterilization to cool
down Quickly. It's necessary to set in each operation depending on objects as follows.
For appliance(except glassware) ••. on
For glassware, liquid or agar medium, other high viscosity substance .•• of F
Even in case of on, automatic exhaust starting temperature can be set according to boiling
point of objects to prevent bumping. Setting range of automatic exhaust starting temperature
is 102 to 121°C.

~CAUTION
o Set automatic exhaust or not in each operation depending on object to be sterilized.
The object which is insufferable under rapid pressure and temperature reduction may burst in chamber in case
of automatic exhaust.

.Manual exhaust

Either automatic exhaust oFF, or automatic exhaust on after sterilization complete, you can
exhaust manually by pressing EXHAUST KEY before the temperature in the chamber reaches to
automatic exhaust starting temperature.
If you press EXHAUST KEY again, you can stop exhaust.

~CAUTION
•• Manual exhaust under 121°C is desirable.
Exhausted steam from the chamber is not cool enough which may cause burn injury.
And water in exhaust cooling tank reduces faster therefore frequent water supply is required.

28
 WON
-' c I
L _'°c DAY
-
I \
STERILIZE
G ~ TIWE
~~
tHEAT
II ·:111
LI· eLl
Ifl WIN
\=~ PATTERN

~ [2J~
~ To the mode of exhaust setting

Change automatic exhaust an/OFF

by CHANGE •.•. KEY.

~[iJ
[2J~
~ Press PATTERN/ENTER KEY to fix the setting.

II
LI
DAY
°c
eXHAUST

~[iJ
[2J~
START
TIMER
on
-:==--..",IflO------..,.W=IN
I

Press PATTERN/ENTER KEY to fix the setting.

Change automatic exhaust starting r-l
temperature by CHANGE.... KEY. -l.-1- EXHAUST LAMP Iighting-up

I C
______ C
__ -_'_°c
I eXHAUST
I S~E

~[iJ \EXHAUST

~::~ I \=\\
110O DAY \

II
LI·CLI
II .:J I HEAT
\'l ~
[\7](
v 1 PATTERN S<!>TAR
T )

Ifl WIN

29
5. 8 Press START KEY
Press START KEY to start operation.
CD The heater is on with "the output lamp of heater" on TEMPERATURE DISPLAY lighting up, and
HEATING-1 PROCESS LAMP blinking.
(2)Mixture of steam and air in chamber is evacuated to exhaust bottle with rise of temperature. This is
the process of filling chamber with saturated steam, not abnormal condition. The mixture steam
stops evacuating at 100°C to 120°C by closing exhaust valve which is controlled by
microprocessor.
When the air evacuation finishes, HEATING-2 PROCESS LAMP blinks as below chart.
Afterwards, temperature and pressure in chamber rise quickly to setting sterilization temperature.
@ When the temperature in chamber reaches to setting temperature, sterilization timer starts to work,
and remaining time of sterilization is counted down on TIMER DISPLAY.
Afterwards, the autoclave keeps the temperature and pressure constantly during sterilization time.
STERILIZA TION PROCESS LAMP and the output lamp of heater blink during sterilization as below
chart.

Start operation.
Press START KEY.
D

The output lamp oL

J C 8 §=~
:~.c
nooST G~rI\l~
~~~
of heater
n . J n e'J)EAT
LI • L LI ~/\
"=,,
\) \) ~
(v] ~
PATTERN

~
~ MIN

D
G ~
I II
I LI
I I
I·c
eXHAUST

1: ~ =
~~~rI\l ~
;, .J;' I ~I/
LI.LLI ~
~AT "=,,
\) \)~
PATTERN (v] ~
~
HR MIN

@ D

emalnlng time 0
ster i I i zat i on

~WARNING
o Do not touch autoclave more than necessary.
If operator touches autoclave more than necessary. it may cause serious accident.

30
5. 9 Operation complete

I " "
I LI I
I
°c
eXHAUST
I ,X:A~TE)
~E -,
~ TIME
~[i]
lION

II · II II
LI• LI LI
DAY

~i~~~ I HEAT
I
\\ r===J \\
\\ \\
PATTERN

~ [7J~
HR WIN

E)
'-----M-:-oN---oAY--'
o " "
LI °c
eXHAUST
I ~E
\EXHAUST
~ TIME

II · II II ~i~~~
II HEAT \r===J \\ PATTERN

LI. LI LI I \\ ~
HR WIN COMPLETE

~ Lighting Lid lock can not be released yet.

D Press STOP KEY.

Extinction: Lid lock can be released.

@ After sterilization time has elapsed, complete alarm sounds then power to heater is cut off. In case
of setting automatic exhaust ON as above chart, automatic exhaust starts after temperature in
the chamber reaches to automatic exhaust starting temperature to decrease pressure forcibly in
chamber.
(In case of automatic exhaust OFF, EXHAUST LAMP does not blink and autoclave does not exhaust
steam automatically. When you press EXHAUST KEY, autoclave exhausts steam.)
COOLlNG-1 PROCESS LAMP blinks from sterilization temperature to 100oe.
After that COOLlNG-2 PROCESS LAMP brinks from ggoe to completion.
@ When TEMPERATURE DISPLAY indicates so-e. complete alarm sounds and COMPLETE LAMP
lights up.
® Press STOP KEY to release the lid lock. Then the lid can be opened and COMPLETE
LAMP turns off.
*In case of interruption of operation, the lid can not be opened till TEMPERATURE
DISPLAY indicates below 80°C too.

31
&WARNING
o Be sure to set automaticexhaust OFF when sterilizing liquid object or glass container.
(Do not press EXHAUST KEY.)
Quick reduction of chamber pressure may cause breakage of glass container or blow-out of hot liquid.
(9 Do not slide the lid lock lever by force nor open lid till TEMPERATURE DISPLAY indicates below
80°C.
Lid lock can not be released. Because it is mechanically-locked. Forcing the lever to move may damage to
mechanical locking device, it may cause unexpected accident.

&CAUTION
o Manual exhaust under 1210C is desirable.
Exhausted steam from the chamber is not cool enough which may cause burn injury.
And water in exhaust cooling tank reduces faster therefore frequent water supply is required.
o Be sure to press STOP KEY to release lid lock after operation complete.
If you don't press STOP KEY, lid lock is not released.
(9 Do not slide the lid lock lever to the right side before you release the lid lock.
The lid lock may not be released which leads to Er4.

32
.Checking operation pattern, value of temperature and time during operation

I' MON
2 51~8
§=~ DAY
G
~[IJ
" . '-LI
L'·
HR
'" MIN
START
TIMER

/'
tHEAT

\
~~~
PATTERN

~ [2J[iJ
Steri Iization
temperature D
" /

I
I ,
c- I
I
°c
8 S?
RIl
W G
~[IJ
[2J[iJ
Warming
temperature D
t: LI
J "
°c
8 I-\ STERILIZE
G
~[IJ
~M\ [2J[iJ
EXHAUST LAMP blinks when setting automatic exhaust ON.
The display returns to previous indication after 5 seconds elapsed.

Above chart shows below setting values at sterilization / warming pattern.

Sterilization temperature 121°C

Sterilization time 20 minutes
Warming temperature 50°C
Warming time 2 hours

33
5. 10 Unload object (below 80°C in chamber)

CDCheck if pressure gauge indicates OMPa and TEMPERATURE DISPLAY indicates

below BO°C.
® Slide the lid lock lever slowly to open position (right end) with pressing front part of
the lid cover.
@ Lift the lid holds at both ends of the lid cover a little.
~---e~~~
z- .
'li_iliilV'
.
=

@ Heat (steam) is blown out of chamber.

Disengage a hand not to get burned. Heat
V~~
@ Catch the handle holders of both ends of the lid cover
in inverted hand, and push the lid up.

@ Unload objects slowly.

~WARNING -------------------,
o Do not open lid until pressure gauge indicates OMPa and TEMPERA TURE DISPLA Y indicates below
80"C.
Steam spouts out of chamber by residual pressure which may cause burn injury.
•• When sterilizing liquid, it takes longer to cool. When opening the lid, pay attention not to get burned
then unload the objects avoiding the impact to them.
liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber.
•• In case of breakage or spill of objects, be sure to clean inside of chamber to use autoclave safely,
or it may decrease life of heating element and/or chamber, then causes corrosion of chamber and
serious accident.

~CAUTION
•• Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove, operator may get burned.
o If you spill liquid when unloading objects, be sure to wipe off it.
It may cause malfunction or trouble of autoclave.

34
5. 11 Close and lock the lid

CD Grip lid hold then close lid slowly.

® Slide the lid lock lever slowly to close position (left end) with pressing front part of the
lid cover.
~WARNING -------------------,
o Do not slide the lid lock lever by force.
Forcing the lever to move may damage to mechanical locking device. it may cause unexpected accident.

5. 12 Turn off power switch(breaker)

CD Drain water in chamber by laying down the lever of drain valve in case autoclave is not
used for a long time.
® Turn off power switch(breaker).

• Stop operation

Follow below steps to stop operation.

CD Press STOP KEY to cool down the chamber.
(Press EXHAUST KEY to shorten cooling time if needed.)
® When TEMPERATURE DISPLAY indicates aooe, complete alarm sounds and
COMPLETE LAMP blinks.
@ Press STOP KEY again to release lid lock then COMPLETE LAMP turns off.
@ After checking if the pressure gauge indicates OMPa, slide the lid lock lever slowly to
open position (right end) with pressing front part of the lid cover. Then, open lid.

~WARNING
IS> Do not open lid until pressure gauge indicates OMPa and TEMPERATURE DISPLAY indicates below
so-e.
Steam spouts out of chamber by residual pressure which may cause burn injury.
o When sterilizing liquid, it takes longer to cool. When opening the lid, pay attention not to get burned
then unload the objects avoiding the impact to them.
liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber.
o In case of breakage or spill of objects, be sure to clean inside of chamber to use autoclave safely,
or it may decrease life of heating element and/or chamber, then causes corrosion of chamber and
serious accident.

~CAUTION
o Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove. operator may get burned.
o Manual exhaust under 121°C is desirable.
Exhausted steam from the chamber is not cool enough which may cause burn injury.
And water in exhaust cooling tank reduces faster therefore frequent water supply is required.

35
5. 13 Other functions

Autoclave has calendaring function. It's necessary to set date and time as follows according
to the location.

5. 13. 1 Date and time setting

Local time has already been set. But this time data will be lost about one month of no
electrical power supply to autoclave. Then calendar is reset to Jan. 1st, 2000 A.D. 0:00. (Er6
indicated)
Set up current date and time as following procedure.

, C EXHAUST STERILIZE
- IQO[1 ~ § ~~
D ~
t:....:::..:..J
C_'·c I
---""'::::::------:o=,y
e
1\ W

" .:J "

LI·CLI
I TIMER ~
START HEAT \=~--r.;;,; [2J ~¢
PATTERN \7
V
START

HR IIIN

To the mode of time Press START TIMER KEY

setting at present . for 5seconds.

. J II II II G [~][1\ 1 ~
L LI LI LI c
eXHAUST

(J= ~ c:: ® TIME

I HR 111M
I @~ / ~AD\
r~~v [¢ 1

Change month,day, hour, and minute r-l Press PATTERN/ENTER KEY

by CHANGE A .•. KEY. ~ to fix the setting.

Press START KEY to fix date and time.

36
5. 13 Other functions

Autoclave has calendaring function. It's necessary to set date and time as follows according
to the location.

5. 13. 1 Date and time setting

Local time has already been set. But this time data will be lost about one month of no
electrical power supply to autoclave. Then calendar is reset to Jan. 1st, 2000 A.D. 0:00. (Er6
indicated)
Set up current date and time as following procedure.

2 51'c8,s=\E G§~~
---MQN:::::-----:D="
n ':J',
LI·CLI
I
1 \=~--r.;;,; ~ ~<l>
TIMER
START HEAT PATTERN \7
V
START

HR MIN

To the mode of time n Press START TIMER KEY

setting at present. ~ for 5seconds.

Change month,day, hour, and minute n Press PATTERN/ENTER KEY

by CHANGE. T KEY. ~ to fix the settin~

o
LI L
J 0
_"c 81/ T
=
~
G~H/\l~
~~®
1/5MQN:)5~1 @I/ ~=~ ~ V ST~T

HR WIN ~EAD{

Press START KEY to fix date and time.

36
5. 13. 2 Reservation operation
Autoclave has function
with calendar timer. If
objects from chamber
can use it as following
of reservation operation which can be set starting time of operation
you set reservation operation for next day, you will be able to unload
next morning. This function save time especially busy morning. You
procedure accordingly.

Make autoclave ready to start operation. (Refer to 5.1 to 5.7)

__
-,I-I 8I
_

L
_

' °c
EXHAUST _
STERILIZE

\ IQ"O:Tl ~~
~

TIME
~ 1\ ~ STOf'
L::,@

,~ • :J ,;Y
u.cu
fYT:: II
,
HEAT \YWARM
~
\ PATTERN
~
(V 1 ~~
~ IIIIN

A time at present is displayed. Press START TIMER KEY

16:46, August 31. to reservation mode.

Press PATTERN/ENTER KEY to change a bl ink Change month, day, hour,minute

in order of month, day, hour, and minute. by CHANGE...... KEY.

r-l Press START KEY to

~ start resevertion operation.

&CAUTION
•• Be sure to check drain valve is closed and water level in chamber before reservation operation.
There is a possibility that autoclave breaks down due to water lack.

37
5. 14 For the reliable sterilization

In case of sterilizing following containers which hinder the circulation and penetration of
steam, the temperature rises slower than temperature in chamber. Because residual air in
following containers is difficult to be evacuated.
Also it may cause Er1 (over pressure error) .

• Containers which hinder the circulation and penetration of steam are;

• Disposable plastic bag
Closed containers
• Deep containers
• Containers with small mouth

Especially, in case of using disposable plastic bag and stainless bucket, refer to
5.4 Load object to prevent sterilization incomplete and/or danger of over pressure.

To operate assured sterilization, be sure to check result of the sterilization by the chemical
indicators and so on.

38
6. Safety device
&WARNING
(S) Do not disassemble or adjust safety valve since it has been adjusted before shipment.
It may cause fatal accident because of abnormal performance.
(S) Do not touch power switch(breaker) by wet hand.
It may cause electric shock.

&CAUTION
(S) Do not pour water into chamber until heater cools down enough after water lack, or it may cause
heater trouble.

6. 1 Safety valve

Safety valve is installed in autoclave to prevent over pressure.

If chamber pressure increases over maximum operating pressure due to any reason, safety valve opens
by itself to decrease pressure in chamber at once. If safety valve activates during operation, please act
on the following procedure.

CDKeep away from autoclave to avoid steam blowing from bottom plate.
® After losing steam, turn off power switch(breaker). Then let chamber cool naturally.
@Contact local distributor. When contacting the distributor, be sure to inform model and serial number.

6. 2 Over pressure detector

Over pressure detector is installed in autoclave to prevent over pressure.
If chamber pressure increases over maximum operating pressure due to any reason, over pressure
detector works, Erl is indicated, and power to heater is cut off to prevent abnormal high pressure.

CDTurn off power switch(breaker).

® Leave the autoclave until pressure gauge indicates OMPa.
@Open the lid. Then, check if the heater is dipped in water.
(Erl is indicated by water lack or over pressure. So check if over pressure happens.)
@If sterilization is incomplete, operate again referring to 7.2 Trouble shooting Q.

6. 3 Water lack preventive device

Water lack preventive device is installed in bottom of chamber to prevent water lack.
If you operate without water in the chamber, water lack preventive device works, Er 1 is
Indicated, and power to heater is cut off. If water lack preventive device works, please act on the
following procedure.

CDTurn off power switch(breaker).

®Leave the autoclave until pressure gauge indicates OMPa.
@Open the lid. Then, check if the heater is not dipped in water.
(Erl is indicated by water lack or over pressure. So check if water lack happens.)
@Leave autoclave as it is at least one hour. Then, pour water into chamber.

39
7. Trouble shooting
~WARNING ----------------,
o If the situation of autoclave correspond to following item(CDor<2) when you ask for repair
autoclave, make autoclave and accessories to be non-polluting condition.
CD Part of autoclave is exposed to infectious substance or radioactive substance.
(2) Blood or other hazardous components for human body adhere to part of autoclave.
(9 Do not touch power switch(breaker) by wet hand.
It may cause electric shock.

~CAUTION
o When water lack alarm sounds, turn off power switch(breaker) and leave autoclave as it is at
least one hour because the chamber is high temperature. Then pour water into chamber, or it
may cause heater trouble.

In case of trouble happening despite you operate correctly following this instruction manual,
check the cause and suitable treatment stated on below table. If you cannot find out proper
article on below table, or if it is difficult to treat, turn off power switch(breaker). Then
contact local distributor and notify serial number of the autoclave.

7. 1 Error list

Error number Error content

ErO Memory error
Er1 Water lack / over pressure
Er21 Control sensor error
Er22 Temperature sensor error
Er3 Over temperature error
Er4 Inter lock error
Er6 Clock error
Er7 Water supply error
Era Printer error
Erg Lack of printer paper

*Refer to 7.2 Trouble shooting about causes and countermeasures of the errors.

40
 7. 2 Trouble shooting
TROUBLE CONDITION
A. Power is not on.
B. Power is on, but the breaker has tripped
immediately.
C. After pressing START KEY, chamber
temperature does not rise.
D. When pressing START KEY, error sound
beeping and operation does not start.
E. The temperature in chamber rises, but it
does not rise to setting temperature.
F. The temperature in chamber rises to the
set temperature, but sterilization has
not been completed due to inside the
chamber does not become filled with
saturated steam.
G. Evacuating residual air does not stop
around 100"C. And the temperature
does not rise.
H. Abnormal noise.
I. Steam leakage from lid.
.-
J. No drainage.
K. Drainage does not stop.
CAUSES
1. Power cable is disconnected.
2. Misconnection of power cable.
3. Burning of connecting part in power
outlet or breaker.
1. Electrical leakage.
1-1. Insulation deterioration of heating
element.
1-2. Water leakage at attaching portion of
hea ting element.
1-3. Water seeping into breaker.
2. Short circuit.
2-1. Short circuit of heating element.
2-2. Short circuit of others.
1. Breakage of heating element.
2. Burning of wire connected to heating
element
1. Completion process of previous operation
is not reset.
1. Wrong electricity supply.
1. Too much capacity of objects in chamber.
2. Shortage of sterilization time.
3. Residual air inside container prevents
penetration of steam.
4. Air evacuation is insufficient due to bent
exhaust hose.
1. Breakage of exhaust valve.
2. This autoclave is installed over 1OOOm
above sea level.
1. Dusts are attached to lid gasket.
2. Lid is out of alignment forcibly.
1. Deterioration of lid gasket.
(cracks in the gasket or hardening)
2. Lid and chamber are out of alignment
caused by lid opened too much or moving
main body holding only lid.
1. Drain pipe is choked.
1. Breakage of drain valve.
(Piece of glass or other objects came into
valve and damaged "Teflon ball" of valve.)
41
COUNTERMEASURES
1. Be sure to connect cable.
2. Be sure to connect cable properly.
3. Contact local distributor.
1. Contact local distributor.
2. Contact local distributor.
1. Contact local distributor.
2. Contact local distributor.
1. Press STOP KEY to reset completion
process of previous operation.
Then press START KEY again.
1. Check the required voltage of autoclave,
then supply proper electricity to the
autoclave.
1. Quantity of objects to be put inside of
chamber shall be less than 60% of capacity
of the chamber. And each objects must
keep enough space between them.
2. Increase sterilization time appropriately
in case of sterilization of liQuid objects
and objects being large Quantity.
3-1. In case of objects being put in a
disposable plastic bag, Quantity of
objects to be put inside disposable
plastic bag shall be less than 60% of
capacity of the chamber. Disposable
plastic bag must have open mouth.
3-2. Containers shall not be superimposed
each other as possible as you can.
Then set its open mouth downward.
4. Do not bend exhaust hose.
1. Contact local distributor.
2. This autoclave can not be used over
1000m above sea level.
1. Stop operation and take away dusts.
2. Contact local distributor.
1. Contact local distributor.
2. Contact local distributor.
1. Contact local distributor.
1. Contact local distributor.
 TROUBLE CONDITION
L. Safety valve activates.
(Steam blowing from bottom plate.)
M. Pressure gauge does not indicate
correct pressure value.
(Pressure gauge does not indicate OMPa
when the lid is opened.)
N. The lid lock lever can not be released
O. Movement of the lid lock lever is hard.
P. ErO is shown on the display.
Q. Er1 is shown on the display with alarm
and operation stops.
(Er1 is shown when water lack preventive
device or over pressure detector works.)
CAUSES
1. Residual air is not evacuated enough and
pressure in chamber rises abnormally due
to additional pressure by residual air.
2. Chamber pressure surpassed set
pressure of safety valve due to the
malfunction of temperature sensor.
1. Deterioration of pressure gauge.
1. Power switch(breaker) is OFF.
2. In operation or chamber temperature is
over 80CC.
3. COMPLETE LAMP lights up after
operation.
1. The lid lock lever is slid by force when lid is
not closed completely.
2. Insufficient space between lid and clamps.
1. Memory error of control unit.
1. Autoclave works without water in
chamber.
2. Drain valve is opened.
3. Breakage of water lack preventive
device or over pressure detector.
4. Over pressure detector activated.
4-1. Residual air in the capacious container
or disposable plastic bag swelled.
4-2. Air evacuation is insufficient due to bent
exhaust hose.
42
COUNTERMEASURES
1-1. Quantity of objects to be put inside of
chamber shall be less than 60% of
capacity of the chamber. And each
objects must keep enough space
between them.
1-2. In case of objects being put in a
disposable plastic bag, quantity of
objects to be put inside disposable
plastic bag shall be less than 60% of
capacity of the chamber. Disposable
plastic bag must have open mouth.
1-3. Containers shall not be superimposed
each other as possible as you can.
Then set its open mouth downward.
1-4. Do not bend exhaust hose.
2. Contact local distributor.
1. Contact local distributor.
1. Turn on power switch(breaker).
2. Press STOP KEY on the condition that the
TEMPERA TURE DISPLA Y indicates below
80CC after operation complete.
3. Press STOP KEY to release the lid lock.
1. Slide the lid lock lever slowly to close
position (left end) with pressing front part
of the lid cover.
(Especially TEMPERATURE DISPLA Y
indicates over 60CC.)
2. Contact local distributor.
1-1. Power off, then power on again.
1-2. If problem still exists after taking above
countermeasure, contact local
distributor.
1. Turn off power switch(breaker) and leave
autoclave as it is at least one hour.
Because the chamber is high temperature.
Then pour water into chamber.
2. Close drain valve.
3. Contact local distributor.
4-1. Containers shall not be superimposed
each other as possible as you can.
Then set its open mouth downward.
In case of objects being put in a
disposable plastic bag, quantity of
objects to be put inside disposable
plastic bag shall be less than 60% of
capacity of the chamber. Disposable
plastic bag must have open mouth.
4-2. Do not bend exhaust hose.
 TROUBLE CONDITION
R. Er210r Er22 is shown on the display
with alarm.
S. Er3 is shown on the display with
alarm.
T. Er4 is shown on the display with alarm.
U. Er6 is shown on the display.
V. Er1 is shown on the display.
(only for automatic water supply
function)
W. Er8 is shown on the display.
(only for printer function)
X. Er8 and Erg are shown on the display.
(only for printer function)
CAUSES
1. Breakage of control sensor or object
temperature sensor.
1. In a sterilization process, temperature
in chamber rises 20C higher than
setting temperature in certain period
of time.
This function is reset after dropping in
temperature within range of ±2OC from
setting temperature.
1. Lid lock lever is not slid to close position
(left end) completely.
2. Lid lock lever is slid by force when the lid
is not closed completely.
3. Lock position jolts out of alignment by
doing same operation as above several
times.
4. The lid lock lever is slid to the right side
before you release the lid lock.
1. Battery for clock shutoff.
2. IC in the control board is broken.
1. Abnormal water supply - Sensor does not
detect water level for 3 minutes
due to the trouble of water supply.
(The city water faucet is closed)
1. The printer breaks.
1. Paper-out condition on the printer.
2. Printer cover is opened.
43
COUNTERMEASURES
1. Contact local distributor.
1-1. The distance between chamber wall and
objects shall be kept more than 10mm.
1-2. Oleaginous objects must not be
sterilized. (Oil causes unexpected rise in
ternperature.)
1-3. Contact local distributor.
1. Slide the lid lock lever slowly to close
position (left end) with pressing front part
of the lid cover.
(Especially TEMPERATURE DISPLA Y
indicates over 60OC.)
2. Slide the lid lock lever slowly to close
position (left end) with pressing front part
of the lid cover.
(Especially TEMPERATURE DISPLA Y
indica tes over 60OC.)
3. If problem still exists after taking above
countermeasures, contact local
distributor.
4. Slide the lid lock lever to the close position
(left end).
1. Turn off power switch(breaker) and turn
on it again. Set the right time.
(Refer to 5.13.1 Date and time setting)
2. If problem still exists after taking above
countermeasure, contact local distributor.
1. Open faucet.
1. Contact local distributor.
1. Set the new paper.
2. Close printer cover.
 8. Maintenance
.Regular Maintenance

Daily maintenance CD Replacement of water

Monthly maintenance
Yearly maintenance
® Check of lid clamping unit
@ Cleaning of chamber and lid gasket
@ Check of power supply and its peripherals
@ Check of water level of exhaust cooling tank z
@ Periodic inspection

8. 1 Daily maintenance
CD Replacement of water
Be sure to replace water in the chamber every day as follows.
Failure to this replacement reduce life span of chamber
due to choking pipes and/or oxidizing water.

• How to replace water

1. Open lid, and empty chamber.
(also base plate, use base plate hook for CL-40L)
2. Unload exhaust bottle from housing hole and draw out exhaust hose from the bottle.
3. Put the chamber drain tray under the drain outlet as below figure.
4. Open the drain valve located at left side of housing hole.
5. Drainage must be carried out several times till no water in the chamber then close
drain valve.
6. If there is foreign object, remove it and load base plate.
7. Pour water to the same level as base plate.

For the drainage

~WARNING -----------------,
<9 Do not open lid until chamber pressure goes down to "OMPa".
Steam spouts out of chamber by residual pressure which may cause burn injury.

44

I -- ~ ~~ _===~====~==~==~==========~_=====~====
_
 L!}. CAUTION
o When replacing water in chamber, check the chamber temperature
cause burn injury.
if it is cold enough, or it may

o over
Empty chamber, drain whole water in chamber, then close lid when autoclave is not used for
a week.
o Beplate.sureIf tothecheck water level in the chamber. In case of lack, pour water up to level of the base
water is impure, replace it.
o Water put in the chamber must be distilled one or purified one. Frequent cleaning of the chamber
is requested when using city water which generates scale in it.
o DoDo not spill water when pouring it. If you spill water, wipe off it.
o not use well water and industrial water which may cause corrosion and/or smudge.
Failure to use proper water may decrease life of the heating element and/or the chamber.
o Drainage must be carried out several times.
Overflow from chamber drain tray may cause burning.
o Use base plate hook when unloading base plate.(CL-40L only)
® Check of lid clamping unit
• How to check
1. Open the lid.
2. When sliding the lid lock lever slowly to the close position (left end), check all clamps
turn around to the locking positions. If the clamps are at locking positions, you can
see them from the slit of the silicon rubber pan. (Fig.1, Fig.2)
3. Slide the lid lock lever to the open position (right end), then close the lid.
4. Slide the lid lock lever to the close position with pressing the front part of the lid
cover, check if the lid lock lever is slid smoothly.
L!}.WARNING
o Do not use autoclave if clamps do not turn around to locking positions.
If you leave clamps unchanged, it may cause fatal accident. Contact local distributor.
o Do not use autoclave if the lid lock lever is not slid smoothly.
If you leave the lid lock lever unchanged, it may cause fatal accident. Contact local distributor.
o Do not open lid until chamber pressure goes down to "OMPa".
Steam spouts out of chamber by residual pressure which may cause burn injury.

Fig.1 CL-40S/ 40M/ 40L (Six clamps)

45
 Fig.2 CL-32S/32L (Four clamps)

8. 2 Monthly maintenance

@ Cleaning of chamber and lid gasket

• How to clean
1. Open lid, and empty chamber.
(also base plate, use base plate hook for CL-40L)
2. Turn off power switch(breaker).
3. Unload exhaust bottle from housing hole and draw out exhaust hose from the
bottle.
4. Put the chamber drain tray under the drain outlet as below figure.
5. Open the drain valve located at left side of housing hole.
6. Remove dusts and foreign object, then wash wall and bottom of the chamber
by water.
7. Wipe off the open mouth of the chamber with a soft cloth containing ethanol.
(Do not use solvent such as thinner.)
8. Wipe off the lid gasket with a soft cloth containing ethanol.
9. Load base plate.

LhWARNING -------------------,
(9 Do not touch power switch(breaker) by wet hand.
It may cause electric shock.
(9 Do not open lid until chamber pressure goes down to "OMPa".
Steam spouts out of chamber by residual pressure which may cause burn injury.
o If you find a crack or hardening on the lid gasket, please contact local distributor.
If you leave crack one unchanged. it may cause burst at worst as well as leakage of steam.

46
 ~CAUTION
~ Do not scratch heater in bottom of chamber when cleaning.
The water which infiltrates into heater may cause breakdown.
o When cleaning, check the chamber temperature if it is cold enough, or it may cause burn injury.
~ Do not pull out the lid gasket forcibly.
It may cause steam leakage.
o Use base plate hook when unloading base plate.(CL-40L only)

@ Check of power supply and its peripherals

• Power connector

1. Make sure if the power cable does not have any damage and there are not
heavy things on the cable.
2. Make sure if the power connector of the autoclave is well connected to the
power receptacle.
3. If the dust is attached with power connector, clean up them .

• Breaker
1. Check if the breaker(power switch) is shut off
by pushing the test button by a sharp object (pen etc.),

~WARNING
~ Do not use autoclave if power cable and/or power connector are damaged.
Contact local distributor, or it may cause electric shock.
o If the dust is attached with power connector, clean up them.
It may cause fire by heat.
~ Do not touch the power connector and breaker by wet hand.
It may cause electric shock.

47
8. 3 Yearly maintenance

@ Check of water level of exhaust cooling tank

• How to check
1. Detach the right side panel
2. Check if the water level of
In case of water lack, pour
4. 5 Standard accessories.
3. Attach the right side panel
using phillips-head screw driver.
exhaust cooling tank has a range of below figure.
water into the tank referring to
t:
using phillips-head screw driver.

II \V

E/I\
E
0
"<t

•....
0

0
C"?

~CAUTION
(9 Do not pour too much water into exhaust cooling tank.
It makes overflow which may cause malfunction and/or breakage of autoclave.
o Be sure to stopple the funnel after pouring water into exhaust cooling tank.
Water in exhaust cooling tank reduces faster therefore frequent water supply is required.

® Periodic inspection

Make sure you inspect the autoclave at least once in a year referring to
"Periodic inspection manual" owned by a distributor in your region.
Failure to this periodic inspection, it may cause not only shortening life of autoclave
itself, but also a fatal accident by a defect of main parts.

*The lid cover and the operation panel may turn yellow due to aged deterioration by
ultra violet.

48
 8. 4 Spare parts and replacement
Refer to the following list and replacement manual of main parts.

8.4.1 Spare parts list for CL series

8.4.2 Replacement of the lid gasket

8.4.3 Replacement of pipe heater

If you will order these spare parts, please contact local distributor.

8.4.1 Spare parts list for CL series

Model No. ModelNo.
No. Part name Photo No. Part name Photo

Main board Main switch ACJOOY~

12
AC240Y
Controller for CL series Earth leakage breaker

SSR AQA411YL
Heater relay
2 13
Solidstate relay for steam heater DC24Y 25A
Main circuit relay

Exhaust valve AC220Y~

Check valve
3 14 CYS-1/4
AC240Y
Solenoid valve with fitting

Lid gasket CL-32 Pressure switch

4 15
Molded silicon rubber CL-40

Steam heater Pressure safety valve

. -
5 16
Sheathed pipe heater ~
Control sensor Rubber pan CL-32
6 17
Resistance PtlOOn, Class B Molded silicon rubber CL-40

Water lack preventer

7 55.13269.010
Thermostat

Pressure gauge
8 GK25-AI8
0.7MPa CL- DP series

Drain valve unit Cartridge filter CCS-020

9
-CIH
Ball valve 0.2!!m

Operation board Air pump

10 G3905-H 2 APNIIO-KEX
Drying pump

Locking device Drain valve AC220Y~

II 3
AC240Y
DC solenoidwith safety switch Solenoid valve with strainer
~
"* Please specify the model name of autoclave and serial number when you order.

49
8.4.2 Replacement of the lid gasket

1. Open the lid.

2. Loosen the nut with open ended spanner 10mm.
3. Pull out the old lid gasket

Assembly

Nut

Out~
• Check that all parts are free from dirt and clean them if
necessary.
Inner lid
Lid gasket

<, Sensitive part

\1/
/
./
<;
• This dimension has to be around equality.

4. Attach the inner lid to the new lid gasket

5. Insert the inner lid to the outer lid and press your palm on center part of the inner lid.
6. Tighten the nut
7. Start the sterilization and check the lid for any leakage.

o A TTEN7l0N: Apply ethyl alcohol that the lid gasket is reassembled in correct position.

50
8.4.3 Replacement of pipe heater

Wiring

1.Dismantlement
1) Pull off the 3pcs. of metal fittings"E" with pliers.
2) Open the both sides of the body and fall down sideways for easy work.
3) Disconnect wiring of the heater.
4) Open assembly nuts of the heater with spanners.(Opposite side 21)
5) Dismount the heater from bottom of the chamber.
6) Take off the sensor"C" from the heater.

2.Assembling
1) Install the new heater on bottom of the chamber.
Meantime, be sure to mount 2pcs. of the gaskets as above drawing.
2) Connect the wiring.

3.Checking
1) Fill water up to the base plate in the chamber and perform the allowable
maximum temperature.
Hereby, check current of the heater.
2) When the chamber temperature is reached, check steam leakage of the
mounted positions.
3) Check dimension and resistance of the Insulator.

51
 (C
•
en
"'C
(t)

Model CL-32S CL-32L CL-40S CL-40M CL-40L _.

o
Heating method Electrical heater in chamber ~
Maximum operating pressure/temperature 0.27MPa/140°C 0.25MPa/137°C
o
Q)
Operating Sterilization 100 to 140°C 100 to 13rC e.
temperature range Dissolving/Warming Dissolving: 40 to 99°C Warming: 40 to 60°C o
Timer Sterilization/Dissolving/Warming: 0 to 48hoursOOminutes
:J
tJ)
AC220/2301240V, 1 cjJ, 50/60Hz
Power source
12A 18A
Chamber dimension l.D{mrn) x D(mm) cjJ320 x 420 cjJ320X676 cjJ400 x 478 cjJ400 x 672 cjJ400 x 832
Capacity 34Q 54Q 60Q 85Q 105Q
Body dimension W(mm) x D(mm) x H(mm) 550 x 550 x 870 550 x 550 x 990 620 x 650 x 880 620 x 650 x 1000 620 x 650 x 1160
Weight 66ke: 71ke: 81ke: 87ke: 90ke:
Chamber/Top plate
SUS
/Base plate/Lid
Material
oi Body Steel with melamine resin
'" Gasket Silicon rubber
Microprocessor controller, graphic display
Temperature: 3digits, PID control
Controller
Timer: 2digits hours / 2digits minutes, down count
(T ernoerature /Time integration system)
Double interlock system, Water lack preventive device,
Safety device Over temperature detector, Over pressure detector, Sensor break indicator,
Memorv of Dower failure Abnormal time indicator Breaker Safetv valve
Hydrostatic test Conformity to the regulation of each country
Safety valve 0.29MPa 0.27MPa
cjJ300 x 200mm cjJ300 x 300mm cjJ380 x 200mm cjJ380 x 300mm cjJ380 x400mm
Stainless wire basket
Zocs. Zoos. Zocs. Zocs, Zocs.
Standard Base plate
Base plate
accessories Chamber drain reciever
Others Chamber drain reciever
Exhaust bottleGl.)
Exhaust bottlaGl.)
Base plate hook

'a "
10. Disposal and dismantlement
Make the autoclave and accessories to be non-polluting condition before disposal and
dismantlement. If the power cable is connected to distribution board directly, beware of electric
shock.

~WARNING
o If the situation of autoclave correspond to following item(CDor®) when you dismantle and/or
dispose of the autoclave. make the autoclave and accessories to be non-polluting condition.
CD Part of autoclave is exposed to infectious substance or radioactive substance.
® Blood or other hazardous components for human body adhere to part of autoclave.
~ Do not disconnect the power cable when the electric power source is turned on.
It may cause electric shock.

53
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55
11. 3 Dangerous substances list

EXPLOSIVE
Nitro glycol, Nitroglycerine, Nitrocellulose, and other nitrate esters

Trinitrobenzene, Trinitroluene, Picric acid, and other explosive nitro compounds

EXPLOSIVE:
Peracetic acid, Methyl ethyl ketone peroxide, Benzyl peroxide, and other organic
peroxides
Sodium azide and other metal azide compounds c

FLAMMABLE
Metallic lithium, Metallic potassium, Metallic sodium, Yellow phosphorus

Phosphorus sulfide, Red phosphorus, Celluloid compounds, Calcium carbide

IGNITING:
Lime phosphate, Magnesium powder, Aluminum powder

Powder of metals other than magnesium and aluminum, Sodium hydrosulfite

Potassium chlorate, Sodium chlorate, Ammonium chlorate, and other chlorates

Potassium perchlorate, Sodium perchlorate, Ammonium perchlorate, and other
perchlorates
Potassium peroxide, Sodium peroxide, Barium peroxide, and other inorganic
OXIDIZING: peroxides
Potassium nitrate, Sodium nitrate, Ammonium nitrate, and other nitrates

Sodium chlorite and other chlorites

Calcium hypochlorite and other hypochlorites

Ethyl ether, Gasoline, Acetaldehyde, Propylene chloride, Carbon disulfide,

and other flammable substances having a flash point of lower than -30 deg. C.

Normal hexane, Ethylene oxide, acetone, benzene, Methyl ethyl ketone, and other
flammable substances having a flash point of -30 deg. C. or higher but lower than
INFLAMMABLE o deg. C.
LIQUID:
Methanol, Ethanol, Xylene, Amyl acetate, and other flammable
substances having a flash point of 0 deg, C. or higher but lower than 30 deg, C.

Kerosene, Gas oil, Oil of turpentine, lsopentyl alcohol, Acetic acid, and other
flammable
substances having a flash point of 30 deg. C. or higher but lower than 65 deg. C.

FLAMMABLE Hydrogen, Acetylene, Ethylene, Methane, Propane, Butane, and other flammable
GAS substances which are assumed a gaseous state at 15 deg, C. and 1 atm

56
Memo

57
 Responsibility

Please follow the instructions in this manual when using this equipment. .:
We have no responsibility for the accidents or breakdown of device if the operator doesn't follow this
manual when operating this autoclave.

Never conduct what this manual forbids.

Unexpected accidents or breakdown may result in.

Note

• The contents of this manual may be changed without notice.

• This manual with missing pages may be replaced.

Instruction Manual for

AUTOCLAVE Laboratory use
CL-32S/32L/40S/40M/40L

Second Edition 12 Oct., 2016

ALP Co., Ltd.

3-3-10, Midorigaoka, Hamura-shi,
Tokyo, Japan
Zip code: 205-0003
TEL: +81 (0)42-579-0531
FAX: +81 (0)42-579-0533
http://www.alpco.co.jp
E-mail: alpco@wonder.ocn.ne.jp

58

1-
 QC-200-S-005-7001E-01

~ CAUTION Commercial cleaning agent

(S) Do not use any commercial cleaning agent for the cleaning of chamber.
It contains acid, alkaline or chlorine which make corrosion of
pressure vessel and its peripherals. This may be cause of premature failure.

Connections on the bottom of chamber which corrode by the cleaning agent.

So that steam leakage happens.

Cleaning agent for pot Citric acid Chlorine cleaning agent

(S) When sodium hypochlorite is used for the cleaning of floor to clean germs,
pay attention not to adhere it to the exterior.
The exterior corrode and become rusty if it adhere to the exterior.

ALP Co., Ltd.