Halophilic bacteria's ability Waste Reduction ..............................

(Nilawati, et al)

K Capacity of B AKTERI H ALOFILIK FOR P ENGOLAHAN L imbah

I NDUSTRI P EMINDANGAN I KAN

A bility H ALOPHILIC B ACTERIA TO T reatment W ASTE W ater FROM S ALT B Oiled

F ISH P rocessing I ndustrial

Nilawati, Marihati, Susdawanita and Nanik Beautiful Setianingsih

Center for Industrial Pollution Prevention Technology
Jl. Ki Mangunsarkoro no. 6, Semarang
Email: nilawatibbtpi@yahoo.co.id

Manuscript received on March 16, 2015, approved on May 6, 2015

ABSTRACT

Industry roomates produced salt boiled fish will get in the waste water processing it. The waste water discharged directly into the
river, it can cause environmental pollution primarily due to the smell of decaying protein. The capacity is 150 m 3 per day for one industry. Waste
water containing boiled fish stew contamination load is high enough, the permanganate value was15,073 ppm and 5.380 ppm BOD.
Wastewater treatment in this study using batch and continuous systems were observed daily for 8 days (days 0, 1, 2, 3, 4, 5, 6 and 7). The
results Showed a Decrease in the value of the most optimal permanganate occurred on day 6 th of 4.266 ppm or decline amounted to 2,401 ppm,
or 36.01 per cent of the batch system. System optimal continuous decline on the 3 rd day ie 5906 ppm down 13:47 percent. So the batch system
is more effective than continuous system at the waste water treatment with salt boiled fish halophilic bacteria. Significantly Decrease BOD
values, for processing by the system as a whole system BOD value is lower than the continuous system. Optimal reduction on day 6 th to 496
ppm of a decline of 1,203 ppm or 70.81 percent, while the continuous system of BOD values ​do not decline.

Keywords: halophilic bacteria, processing, waste water boiled salt fish

ABSTRACT

Pemindangan industry produces fish in the treatment process of wastewater. The waste is directly discharged into the river, it can
cause environmental pollution, especially odor resulting from spoilage protein.Kapasitasnya 150 m 3 per day for one industry.
Liquid waste
boiled fish stew containing contaminant load is quite high, with a value of permanganate
15,073 ppm and 5,380 ppm.Pengolahan effluent BOD in this study using the system
batch and continuously observed daily for 8 days (days 0, 1, 2, 3, 4, 5, 6 and 7). The results showed a decrease in the value of the most optimal
permanganate occurred on day 6 of
4,266 ppm or decline amounted to 2,401 ppm, or 36.01 percent of the system batch. Continuous system optimal reduction on the 3rd day ie
5906 ppm down 13.47 persen.Jadi system batch
more effective than continuous system at sewage treatment preserved by halophilic bacteria. BOD value of a significant decrease, for
processing by the system batch the overall value of BOD system batch lower than the continuous system. The decline optimal ke6menjadi day
decline of 496 ppm 1,203 ppm or 70.81 percent, while the continuous system of BOD values ​do not decrease.

Keywords : halophilic bacteria, processing, waste pindang

PRELIMINARY is the process pemindangan. There are many other fish

preservation process that shelf-life
Fish is a food source of protein that is easily damaged Longer like
so we need preservation, one of them manufacture of salted fish, dried fish, presto and smoked fish. The
process of making boiled fish

23
Journal of Industrial Pollution Prevention Technology Research Vol. 5, No. 2 November 2014 (23-28)

is first washed with water river fish that have previously been research also Ali, I., et al , 2014,
precipitated by alum. After washing the fish are arranged in a using obligate halophilic aspergillus for waste water remediation
basket containing 2-4 tails salted. However, wastewater treatment pemindangan fish with
halophilic bacteria has never been done.
then fish basket
containing boiled fish with boiled water that has been added krosok Halophilic bacteria have power
salt with water and salt ratio 3: 1 for 15 minutes at a temperature of resistance and tolerance to contaminants in the surroundings.
100 0 C. then cooling and draining. Boiling water is a liquid waste, These bacteria belong
cooled one night and then thrown into the river (informsi of boiled archaea (commonly called halophilic archaea or haloarkaea),
fish processing industry TPI-2 Bayumulyo Pati, Central Java). This including the family Halobacteriacea,
can cause (Facciotti, M., 2013)
differences in microbial halotoleran
with halophilic is the ability to live in salt concentration range
0-20% NaCl and can live without salt. While the extreme halophilic
pollution environment microbes can thrive only in a narrow range of salt concentrations
especially due to the smell of decaying protein. The test results (eg halophilic species were only able to live in a range of 5 -10%
effluent TSS boiling water is 580 mg / l, sulfide 1.589 mg / l, fatty NaCl) and can not live without salt.
oils
90.0 mg / l, COD 16,800 mg / l, Value
15,073 permanganate and BOD 5.380 mg / l. Small industrial grouping microbes
fisheries in Central Java process pemindangan are some areas like halotoleran be distinguished by
Batang, Tegal, Cilacap and Apex. tolerance to salt concentration is halotoleran low (approximately
1% w / v NaCl), moderate (6-18% NaCl) and high / extreme (18
Most of the
they have not manage wastewater. This is due to limited - 30% (saturated) NaCl) (Simpson, A., 2013; Plemenitas, A., et al, 2013)
knowledge and cost. Research has been conducted by Nasuka, When
The researchers also viewed
et al (1995) with the enormous potential of moderate halophilic bacteria and
use bacterium anaerobic for halotoleran in its application as
process water waste which results pendegradasi organic pollutants.
obtained, imbah water boiled fish decreased by 47.46% organic Ability bacterium moderate halophilic
ingredients and with activated sludge 50.39% decline in organic Marinobacter hydrocarbonoclasticus in degrading some aliphatic
matter. Results of the study also said that the inhibition of microbial and aromatic hydrocarbons (Manjoubi, M.,
growth due to the high content of NaCl waste water so that the et al,
microbes die all. 2013).
Research Irshad, A., et al, ( 2014),
halophilic bacterial diversity in soil waterfront of
based on research earlier Daecheon, Chungnam, and
bacteria do not survive on fish waste pindang so in this study were Saemangeum, Jeonbuk, Korea obtained
selected bacteria halophilic used for wastewater treatment leftover more than 200 halophilic bacteria isolated were not dimanaatkan.
stew pemindangan fish, with consideration for the bacteria
halophilic resistant to high salt so that the condition of the waste Differences in the ability to live in an environment
water with high salt content can degrade organic materials that different salinity between hydrophilic extreme, moderate and
exist the waste water, thus the expected BOD, COD halotoleran showed how to adapt differently. Moderate and
(permanganate value) can down load cemarannya. So in this study halotoleran holofilik bacteria have the ability to balance life
the method of processing waste water containing high chloride
which is very suitable to use
osmotic pressure in order
to avoid the influence of denaturation by salt environment is by
way of salt accumulation and osmolit (organic molecules) in the
cytoplasm (DasSarma, S. and DasSarma,
bacterium halophilic.
Peelitian-research on halophilic bacteria have been done as P., 2012). While archaea and bacteria adapt to extreme hydrophilic
research conducted by Marihati, et al, 2013; 2014. The use of only through the accumulation of high salt concentrations
bacteria halofiik to pembuatn salt in order to improve the purity of (especially KCI) in the cytoplasm (Oren, 2013).
NaCl salt. However peneitian carried out by Sharghia, E., A., et al, 2014,
receipts halofiik bacterial consortium in bioreactors for water peneltian this aims for
treatment hipersalinitas. get the data to the extent of halophilic bacteria capable of
degrading wastewater in industrial pemindangan fish stew fish.

So

24
Halophilic bacteria's ability Waste Reduction .............................. (Nilawati, et al)

RESEARCH METHODS Data analysis

This study uses a variable time of observation consists of
This research was conducted at the Laboratory of Biology, the to-0, 1, 2,
Laboratory of Water and Food and Beverage Laboratory in the 3, 4, 5, 6, 7 with the system batch and continuously. Parameter
Center for Industrial Pollution Technology Pencgahan Semarang in analyzed value
2014. permanganate with titimetri method (SNI 0669809-22-2004) and
BOD by the method of Winkler. Reasons for using value analysis
Materials and tools permanganate
Materials used are waste because waste pindang
boiling boiled fish obtained from boiled fish industry TPI containing high Cl, faster analysis and chemical waste analysis
- 2 Village Bayumulyo, results cemarannya safer than using an oxidant bicromat. while the
Pati regency, NaOH, halophilic bacteria, chemicals for chemical value of
analysis (BOD and permanganate value). permanganate
using oxidizer KMnO 4. The test results obtained were analyzed
The tool used is a glass beaker, erlemeyer, descriptively.
jar test, measuring cups, hot plate, pH
meter and tools for chemical analysis. RESULTS AND DISCUSSION Characteristics of
Wastewater Pindang
metod e
How to Research The results showed fish stew boiled liquid waste contains
Waste water boiled fish stew derived from the boiling many organic compounds. This organic compound is a chemical
process the fish with salt. The following waste water treatment compound with the main element that binds the C atoms to form
process stew boiled fish with halophilic bacteria using the system batch molecules. To determine the organic compound can be done by
and continuously in the laboratory, namely: first with system batch, reactor testing the value of permanganate and BOD. The test results of
with a capacity of 10 liters of activated sludge danair added as waste water boiled fish stew derived from
much as 1 liter and halophilic bacteria as much as 10 percent of

pemindangan industrial fish in TPI-2-Pati Bayumulyo

then aerated fluid volume. Next cemarannya burden remains very high. From the observations and
waste added pindang information from the
up to 70 percent of the reactor volume up to 7 liters of fluid. The all industrial wastewater
remaining 30 percent for aeration space so as not to spill when the langsug dumped into the river. When compared with water quality
aeration process berlangsung.Aerasi standards fishery waste
done up 8 Ministerial regulation Environment No. 5
day.And dilakukansetiap day sampling system batch samples were 2014, then all the parameters are not eligible, the value is very far
taken from the aeration reactor. Second , with a continuous from the threshold except pH eligible. Results of the analysis
system, where the process is almost the same as the system presented in Table 1.

batch, were first added activated sludge

, water and halophilic bacteria as much as 10 percent of the liquid Table 1. Wastewater Analysis Results Stew Pemindangan
Fish
volume of 7 liters and then aerated. Further added pindang waste
Parameter Unit Analysis results BMAL *
up to 70 percent of the fluid volume to 7 liters. Pindang sewage
flowed continuously from the waste water tank is connected with a COD ppm 16 800 150

boiled stew value ppm 15 073 -

permanganate
hose with BOD ppm 5380 75
1 drop per second speed of up to 7 days Sampling was done every TSS ppm 580 100
day where samples were taken from the tank after aerated. Further H2S ppm 1.589 1
analysis permanganate and BOD values ​every day. For halophilic Cl ppm 113 569 1
bacteria nutrient solution diberkan Luria Berthani (LB) the
pH 5-6 6-9
composition Trypton 50 grams, 25 grams of yeast extract dissolved
fatty oils mg / l 90.0 15
in 1 liter of distilled water and then heated at a temperature of
60-70 0 C to dissolve but do not need to boil. LB nutrient usage as
protein % 1.60 -
much as 1 percent.

* Wastewater Quality Standard Fisheries Processing activities (LH

Candy No. 5 of 2014)

25
Journal of Industrial Pollution Prevention Technology Research Vol. 5, No. 2 November 2014 (23-28)

Results of Liquid Waste Processing Industry Pemindangan that is able to adapt to the waste solution boiled with 113. Cl
Fish Stew with halophilic Bacteria content of 569 ppm was able to adapt in the waste preserved so
that it can degrade organic compounds, as shown by the decline in
value Permanganate and
The experimental results boiled sewage treatment
using halophilic bacteria
BOD. Overall happen
a significant reduction, the analysis results presented in Table 2
and Figure 1.

Table 2. Addition influence halophilic bacteria against Permanganate Value and BOD with Batch system and
continuous

Staying
Time Permanganate value BOD pH Sludge Volume (%)
(days)
Batch Continuous Batch continuous batch continuous batch continuous

0 6667 6825 1699 1689 8.7 7.4 25 25

1 7678 8155 1405 3340 6.2 8.1 20 23
2 7678 6749 1380 4061 7.3 8.2 20 20
3 6114 5906 1198 3909 8.1 8.0 20 20
4 5673 6468 1026 4099 8.4 8.1 12 15
5 5261 7031 1310 6793 8.6 7.6 12 15
6 4266 7593 496 6148 8.7 7.6 11 10
7 4550 10968 1026 3883 8.7 7.7 10 7

waste 15073 15073 5380 5380 5.5 5.5 0 0

pindang

Value permanganate to a solution of 3,318 ppm halophilic bacteria stater stater solution
halophilic bacteria and activated sludge amounted to 2,995 ppm

Impairment Permanganate with still adapting activated sludge and

halophilic bacteria halophilic bacteria permanganatnya value of 3,379 ppm and 3,291
ppm BOD with MLSS 1,200 ppm where the mud and water of 850
Results of boiled fish stew of sewage treatment using bacteria ml and 100 ml stater halophilic bacteria and nutrients LB 50 ml,
halophilic then
showed that a decline in the value of the permanganate and BOD the next day
wastewater, p this pindang waste added up to 7 liters for the reactor capacity of 10
proven that halophilic bacteria can liters, the rest of the aeration space so as not to spill when the
adapt and degrade compound- aeration process takes place. Aerated more or less 2
compounds present in the waste water which boiled stew such
waste amounted to 1.60 percent protein so that the protein source Hours later taken
of nutrients for perumbuhan make halophilic bacteria. Bacteria sample, is considered day-to-0. Then measured permanganate
holofilik moderate and halotoleran have value obtained on day 0 is 6667 ppm. The result was compared
with the value of permanganate day 0. The results showed in the
1st and 2nd
ability life for
balancing osmotic pressure in order happen increase in value
to avoid the influence of denaturation by salt environment is by permanganate, p this occurs possibility
way of salt accumulation and osmolit (organic molecules) in the in the sample entrained fish flakes and
cytoplasm (Oren, 2003; DasSarma, S. and DasSarma, P., 2012.). fish oil from wastewater. Day 3 to day-to-6 decreased the most
optimal permanganate value by 36.01 percent (from

Factors important that 6667 became 4.266 ppm), but the 7th day happen again rise up to
affect the growth of bacteria, among others: the supply of nutrients 4,550 ppm, possibly on the 7th day there are some bacteria that
which are carbon, nitrogen, hydrogen, oxygen, sulfur, phosphorus, are already dead so the material can no longer mendengadasi
iron and small amounts of other metals, temperature, pH and organic-materials
oxygen availability (Todar, K., 2004).
case this caused in
The results of this study indicate when the condition halifilik bacterial life requires higher levels of potassium for stability
has not added pindang waste,

26
Halophilic bacteria's ability Waste Reduction .............................. (Nilawati, et al)

and activities (Oren, A., 2003). Here it can be said the bacteria percent, while the continuous system BOD value increased from
had started to die with the support of the data on the number of 1.699 ppm to
bacterial biomass as measured by sludge volume formed already 3,883 ppm on day 7. So boiling water sewage treatment optimal
reduced the initial 25 percent to 10 percent, boiled fish that day 6 dengm batch system.

as presented
Table 2. Possible elements of potassium insufficient or has been
reduced. For continuous system value is higher than using the
system batch, this is because the system batch bacteria adapt to the
environment in solution longer. With the continuous system optimal
reduction on the 3rd day ie 5906 ppm, day 4 to 7 there is an
increase of up to 10 968 ppm, is presented in Figure 1. This is
caused by the amount of biomass are reduced due to the bacteria
disappear ( wash-up)

together with the waste that has been aerated ending up in a tank,
whereas in the system batch the 7th day of 4550 ppm. so the
system batch more effective than continuous system in boiled
sewage treatment.

Figure 2. Influence of halophilic bacteria

BOD wastewater to the Fish Stew Pindang

CONCLUSION

Liquid waste boiled fish stew

still contains a fairly high contaminant load, with a value of
permanganate 15,073 ppm, 5,280 ppm BOD, so in this study
using halophilic bacteria produce process conditions:

1. Value permanganate happen reduction

until the 6th most optimal, reaching 4,266 ppm or decline of
36.01 percent for the batch system. With the continuous
system optimal reduction on the 3rd day ie 5906 ppm
(decrease

Figure 1. Influence of halophilic bacteria
against Permanganate Value Liquid Waste Pindang Fish
Stew
13.47 percent).
2. BOD optimal reduction at day 6 with a 70.81 percent decline.
Continuous system does not decrease.

The decrease BOD with halophilic Bacteria

so the system batch more effective than continuous system
in boiled sewage treatment.
In this study BOD values ​obtained were compared with
the value of BOD day 0. The results showed that from day 1
decline until day 6, as presented in Figure 2. Values ​significant
THANK-YOU NOTE
decrease BOD, for processing by the system batch lebiih overall
Our thanks goes to Mr. Ir. Sartamtomo, sister VIARA
BOD value lower than the continuous system. The initial value of
and Agista who have helped in the implementation of this study.
1699 ppm BOD ke0sebesar days after being processed in a batch
for a residence time of 8 days

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