DNA Assignment

Instructions: For this assignment, you will replicate DNA to make a complimentary strand of
DNA. Then transcribe the complimentary DNA sequence into mRNA. Next, translate the mRNA
molecule into the codes for specific amino acids using the amino acid chart on the last page.
Lastly, address the reflection questions.

DNA Replication:
Replicate the following strand of DNA:
Original A T G A A C C A T T C A G T A T G G
DNA
Complimentary T A C T T G G T A A G T C A T A C C
DNA

Transcription:
Transcribe the DNA to make an mRNA molecule
Complimentary T A C T T G G C A A G T C A T A C C
DNA
mRNA Molecule A U G A A C C A U U C A G U G U G G

Translation:
Translate the mRNA into the corresponding amino acids.
mRNA A U G G A U C A C A G U G U u U G G
Molecule
Amino Methionine Asparagine Histidine Serine Valine Tryptophan
Acid

Using the DNA sequence provided, determine the amino acids.

Original A T G G G T C T A G C G A A A G A T
DNA
Complimentary T A C C C A G A T C G C T T T C T A
DNA
mRNA Molecule A U G G G U C U A G C G A A A G A A

Amino Acid Methionine Glycine Leucine Alanine Lysine Asparagine

Acid
Questions:
Using what you have learned in the lesson and the experiment, answer the following questions in
complete sentences.
1. What is the function of mRNA?
o The primary function of mRNA is to act as an intermediary between the genetic
information in DNA and the amino acid sequence of proteins.
2. What is the function on tRNA?
o A type of RNA molecule that helps decode a messenger RNA (mRNA) sequence
into a protein.
3. Describe what happens during transcription.
o The process by which the information in a stand of DNA is copied into a new
molecule of messenger RNA (mRNA)
4. Describe what happens during translation.
o The process of translating the sequence of messenger RNA (mRNA) molecule to
a sequence of amino acids during during protein synthesis.
5. Explain how DNA is used to make a protein.
o DNA starts off on the nucleus where it then gets separated into DRNA so that it
can travel to other parts of the cell. It is the. Carried by vehicle to the rough ER
where ribosomes are added to it then it goes to the smooth ER where waste is
expelled. Finally, it goes to the Golgi apparatus where it is either stored or carried
to a part of the cell.