Med. Mico. Lab Final_6.

Differential Staining for Visualization

of Bacterial Cell Structures
Terms in this set (8)

Heat penetrate into the endospore wall. Spores have imperiate

Why is heat necessary in spore
coat around them. Coat loosen up the cortex for it to accept
staining?
the stain.

Explain the function of water in spore It wash off the excess primary stain but not the spore.
staining.

*Assume that during the performance The acid-alcohol will removes stain, bacteria, and spores.
of the spore-staining exercise, you
made several errors in your spore-
staining procedure. In each of the
following cases, indicate how your
microscopic observations would differ
from those observed when the slides
were properly prepared: You used
acid-alcohol as the decolorizing
agent.

*Assume that during the performance The cells will stain green and the spore will stain red
of the spore-staining exercise, you
made several errors in your spore-
staining procedure. In each of the
following cases, indicate how your
microscopic observations would differ
from those observed when the slides
were properly prepared: You used
safranin as the primary stain and
malachite green as the counterstain
*Assume that during the performance The stain wouldn't be able to penetrate the spores.
of the spore-staining exercise, you
made several errors in your spore-
staining procedure. In each of the
following cases, indicate how your
microscopic observations would differ
from those observed when the slides
were properly prepared: You did not
apply heat during the application o
the primary stain.

It prevents the white blood cells to phagocytize the bacteria

Explain the medical significance of a and increases virulence.
capsule.
The capsule also inhibits antibodies and complement fixation.

Explain the function of copper sulfate It washes the purple primary stain out of the capsular material
in this procedure without removing the stain bound to the cell wall

1. Flood smear with malachite green and steam over a beaker

of water on a hot plate. Add filter paper over the streak to
prevent evaporation of malachite green.
2. Cool slide and wash stain off with tap water. The water also
Explain the spore-staining procedure.
serves as a decolorizing agent.
3. Counterstain with safranin for 30 seconds.
4. Wash off the safranin with tap water.
5. Blot the slide with bibulous paper.