Class : Agroteknologi B

Topic No. : 01
Group No. : 01

List of Authors Contribution

Person In Charge
Job Description
(tuliskan nama lengkap anggota yang bertugas)
Draft composing (pengarah Ega Akhsanul Fikri (20180210061)
:
sistematika penulisan)
Data composing (pengarah Yoga Adi Wijaya (20180210091)
:
penyajian data hasil)
Translator (penerjemah) Farah Nurma Faedah (20180210055)
:
Ika Lufiana (20180210073)
Literature reviewer Melina Arzi Hukama (20180210077)
:
(peninjau literatur) Asri Tri Wulandari (20180210063)
Draft editor (translation Ahmad Arya Mudawy (20180210099)
:
and grammar check)

RESULTS AND DISCUSSION

1. Title of Research
THE EFFECT OF SUBSTITUTION MEDIUM AND SUBCULTURE EXTRACT ON
Vanda tricolor SUBCULTURE
2. Table 1. Effect of Substitution Medium on Percentage of Live Explants, Percentage of
Explant Contamination, and Percentage of Browning Explants in Subculture Shoots of
Vanda tricolor 8 MST.
Life Contamination Browning
Treatment
(%) (%) (%)
Medium VW + 150g / l Banana Extract 100 0 0
Medium VW + 150g / l Banana Extract + 150 g
79 11 10
/ l Sprouts Extract
Leaf Fertilizer Medium + 150 g / l Banana
100 0 0
Extract
Leaf Fertilizer Medium + 150 g / l Banana
87 0 13
Extract + 150 g / l Sprouts Extract
Medium POC + 150g / l Banana Extract 78 22 0
Medium POC + 150g / l Banana Extract + 150 g
89 0 11
/ l Sprouts Extract
In table 1, treatment medium VW + 150g / l banana extract and leaf fertilizer medium +
150 g / l banana extract, where the percentage of life was 100% and there was no contamination
and browning. Medium VW is the basic medium often used in in vitro culture are orchid plants.
This media consists of macro and micronutrients. Meanwhile, daus fertilizer medium and POC are
also included as basic media. Each medium in the treatment received additional nutrients such as
banana extract and sprouts. These nutrients can promote plantlet growth. Natural or vegetable
substances are generally a source of sugars, vitamins, growth regulators, and amino
acids(Djajanegara, 2010).

However, in plant tissue culture techniques, there is a major problem, namely the frequent
contamination of the media. This results in stunted explant growth, rot, and even death (Leifert &
Cassells, 2001). Contamination is a condition of the cultural environment that is disrupted due to
the entry of contaminants, both fungi, and bacteria (Adihaningrum & Rahayu, 2019). Fungi and
bacteria that enter the culture environment, come from various sources, one of which is plant
explants. Plant explants that come from mother trees that come from nature carry spores and seeds
of fungi and bacteria. So that when the explants are planted, the spores and seeds of the fungus
and bacteria grow and damage the explants.

3. Table 2. Effect of the type of substitution medium on the height increase of Vanda tricolor
shoots. The values displayed are the mean values of 5 replications ± SD (standard deviation).

High gain
Treatment
(mm)
Medium VW + 150g / l Banana Extract 3.8 ± 0.9 a
Medium VW + 150g / l Banana Extract + 150 g / l Sprouts
4.9 ± 0.8 a
Extract
Leaf Fertilizer Medium + 150 g / l Banana Extract 4.2 ± 1.2 a
Leaf Fertilizer Medium + 150 g / l Banana Extract + 150 g /
3.1 ± 0.6 ab
l Sprouts Extract
Medium POC + 150g / l Banana Extract 3 ± 0.5 ab
Medium POC + 150g / l Banana Extract + 150 g / l Sprouts
2.4 ± 0.5 ab
Extract
Medium POC + 150g / l Banana Extract + 150 g / l Sprouts
2.4 ± 0.5 ab
Extract
The number followed by the same lowercase letter is not significantly different according
to the DMRT at the 5% real level.

In table 2, the highest increase in explant height was obtained in the treatment of VW
medium + 150g / l banana extract + 150 g / l sprouts extract, but there was no significant difference.
The green bean sprouts extract can be an alternative application of growth hormone in plants. As
with green beab, cowpea and peanut sprouts also have the potential to be used as phytohormones.
The growth hormone content in the sprout extract differentiated the high increase in the treatment
medium VW + 150g / l banana extract + 150 g / l sprouts extract. (Fitri et al., 2004). The results
of the report (Nova et al, 2017) explained that the peanut sprouts extract contained the highest IAA
hormone auxin (4.40%) and the lowest cowpea sprouts extract was 2.75%.

4. Table 3. Effect of the type of substitution medium on the increase in the number of Vanda
tricolor leaves. The values displayed are the mean values of 5 replications ± SD (standard
deviation).

Number of leaves
Treatment
(sheet)
Medium VW + 150g / l Banana Extract 1.3 ± 0.4 a
Medium VW + 150g / l Banana Extract + 150 g / l Sprouts
0.7 ± 0.2 b
Extract
Leaf Fertilizer Medium + 150 g / l Banana Extract 1.4 ± 0.2 a
Leaf Fertilizer Medium + 150 g / l Banana Extract + 150 g /
0.8 ± 0.2 b
l Sprouts Extract
Medium POC + 150g / l Banana Extract 0.7 ± 0.3 b
Medium POC + 150g / l Banana Extract + 150 g / l Sprouts
0.7 ± 0.1 b
Extract
The number followed by the same lowercase letter is not significantly different according
to the DMRT at the 5% real level.

Based on the results of the study, giving substitution medium treatment affected the
addition of Vanda tricolor leaves. The treatment of Medium VW + 150g / l Banana Extract and
Medium Leaf Fertilizer + 150 g / l Banana Extract was not significantly different according to
DMRT. Meanwhile, when the treatment was added with Sprouts Extract, the results were shown
to be significantly different according to DMRT. The other results of the treatment added with the
respective sprouts extract were not significantly different according to DMRT. Sprout extract
which contains growth hormone also affects the number of Vanda tricolor leaves.

5. Bibliography
Adihaningrum, H. & Rahayu, T. (2019). Potential Biocide of Kepok Banana Leaf Powder in
In Vitro Culture of Black Rice Seeds. In the 4th National Seminar on Biology and Science
Education (SNPBS), 133.
Djajanegara, I. (2010). Utilization of Banana Fruit and Coconut Water Waste as Media for the
Moon Orchid (Phalaenopsis amabilis) Type 229 tissue culture. Journal of Environmental
Technology, 11 (3), 373–380.
Fitri, AM, Solichatun, Musdyantini, & Widya. (2004). Effect of Mung Bean (Vigma radiata
L.) Plant Extracts on Growth and Nodulation of Soybean (Glycine max L.) Journal of
Biosmart, 6 (1): 24-28
Leifert, C. & Cassells, AC (2001). Microbial hazards in plant tissue and cell cultures. Journal
of In Vitro Cell. Dev. Biol. Plant, 37: 133-138.
Hutami, S. (2008). Browning Problems in Tissue Culture. Journal of AgroBiogen, 4 (2): 83-
88.