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ABSTRACT
Fasciolosis caused by Fasciola hepatica and Fasciola gigantica is a zoonotic helminth infection of
ruminants and causes of an important economic loss. The cross-sectional study was conducted from
February to April 2012 at Lemo district and Hossana municipal abattoir to determine the prevalence
of fasciolosis, identify the predominant Fasciola species and to determine fluke burden, severity of
liver pathological lesions of cattle slaughtered and assess the direct economic loss because of bovine
fasciolosis due to liver condemnation. In this study, the sample population of 384 cattle from the
Keywords Peasant Associations were randomly selected and coprologically examined by sedimentation
techniques and 384 cattle were selected by systematic sampling techniques for postmortem
examination at Hossana municipal abattoir. The overall prevalence of bovine fasciolosis on farm and
Abattoir, abattoir study was 34.9% and 30.5%, respectively. The infection rate of bovine fasciolosis on farm
Bovine, and abattoir study was significantly associated (p<0.05) with age, breed, body condition, origin of
Economic study animals as well as the type of Fasciola species, liver pathology and worm burden. However,
loss, there was no statistically significant association of bovine fasciolosis with sex of cattle (p>0.05).It
Fasciolosis, was noticed that a higher (37.5%) prevalence rate was identified in young than adult (33.3%) cattle
and higher (61.9%) in Holstein than cross (35.7%) or indigenous (33.1%) breed. Cattle with thin body
Hossana, condition were higher in infection rate than average or fat. The predominant Fasciola species
Lemo, identified was Fasciola hepatica (55.6%) followed by Fasciola gigantica (19.6%).The average mean
Prevalence fluke burden was 27.48 flukes per affected liver. The mean fluke burden in mixed Fasciola species
(34.00 ± 6.255) was higher and least in Fasciola hepatica (27.85±3.620).The mean fluke burden that
contributed for the liver pathological lesion categories of moderately, severely and lightly affected
livers was (34.37±4.369), (26.76± 2.712) and (7± 0.513), respectively. A direct economic loss
identified in cattle due to liver condemnation by fasciolosis at Hossana municipal abattoir was
estimated 88,806.85 Ethiopian birr Per annum. Bovine fasciolosis was a prevalent parasitic disease
and cause a considerable economic loss in the study area. Therefore, the role of host factors (age, sex,
breed and body condition) and conducive environment for the development of intermediate host snails
should be clearly recognized by all stakeholders in order to understand their effects on the disease
occurrences as well as in control and prevention of fasciolosis in cattle.
Introduction
Fasciolosis is an important parasitic two species most commonly implicated, as
disease of domestic ruminants caused by the etiological agents of fasciolosis are
digenean trematodes of the genus Fasciola Fasciola hepatica and Fasciola gigantica
commonly referred as liver flukes. The (Lotfy et al., 2002). It is a serious disease
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of herbivorous animals (Torgerson and major cause for the considerable economic
Claxton, 1999), leading to huge economic losses in the cattle industry, mainly
losses in livestock production, while through mortality, liver condemnation,
human infection has long been seemed to reduced production of milk, meat and
be accidentally (Mas-Coma et al., 2005). expenditures for anthelmintics (Hillyer
The distribution of Fasciola hepatica is and Apt, 1997). Therefore, the objectives
limited to temperate areas and high land of of this study were to assess the current on
tropical and sub-tropical regions while farm and abattoir prevalence of bovine
Fasciola gigantica is wide spread in most fasciolosis and associated economic loss in
parts of tropical Africa. Thus, the Lemo district.
distribution of two Fasciola species
overlap in many African and Asian Materials and Methods
countries and sometimes in the same
country, although in such cases the Study Area
ecological requirements of the flukes and
their snail intermediate host is distinct The study was conducted at selected areas
(Mas-Coma et al., 2005; Walker et al., of Lemo district and Hossana municipal
2008). abattoir of Hadiya Zone, Southern
Ethiopia. Hadiya Zone is one of the 13
The geographic distribution of Fasciola Zones in the southern Nation Nationalities
species is dependent on the distribution of of Ethiopia. The highest altitude in the
suitable species of snails such as Lymnae Zone is 2970 m.a.s.l at the Summit of
natalensis and Lymnae truncatula, the Sengiya Mountain in the Duna district and
most common intermediate hosts and the lowest is 800 m.a.s.l in Gibe River
usually associated with herds and flocks valley. The zone is divided into ten
grazing wet marshy land area. Both districts and one city administration. Lemo
Lymnae species are needed for the district is one of ten administrative
parasite s life cycle to be completed. districts found in Hadiya zone and located
According to Thomas (1883) and Brown at a distance of 230 km in the North East
(2005), the distribution of fasciolosis is from Addis Ababa and 187 km from
associated with the favorable climatic and Hawassa, the capital city of the region.
ecological conditions for development,
spread and maturity of parasite and it s life According to the information obtained
cycle stages in various areas. In view of from Hadiya Zone Agricultural Office,
the worldwide spread, occurrence and mixed farming system (livestock and crop
zoonotic nature, fasciolosis has emerged production) is widely practiced. The
as a major global and regional concern district consists of 35 Peasant Associations
affecting all domestic animals and and has a total of 137, 889 cattle
infection is most prevalent in regions with population of which 12,066 cattle are
intensive cattle production (WHO, 2008). found in the study areas of four selected
From the many parasitic problems of farm Peasant Associations namely, Ambicho,
domestic animals, fasciolosis is the most Belessa, Shecha and Lereba were selected
important disease, which causes direct and purposively by considering their cattle
indirect economic loss on livestock populations, availability of suitable
production, particularly of sheep and cattle breeding site for intermediate host and
(Keyyu et al., 2006). The disease is the transport access. The study area located
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Fecal samples were collected directly from II. Types of infection: are classified as
rectum of randomly selected cattle by Fasciola hepatica, Fasciola gigantica,
hands protected by rubber gloves, using mixed Fasciola species (Fasciola hepatica,
two fingers (i.e., middle and index Fasciola gigantica) and juveniles.
fingers). Each sample was clearly labeled III. Severity of liver pathological
with animal s identification, date and lesions: Categorization of affected livers
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of bovine fasciolosis in the present study animals in which acquired immunity was
was lower than the previous findings of well developed through repeated challenge
50.98% by Dejene (2008) The present of the disease (Ogunrinade and Adegoke,
study also showed higher prevalence of 1982; Kuchai et al., 2011; Mungube et al.,
bovine fasciolosis as compared to the 2012).
25.46% by Khan et al.(2009). The
variation in overall prevalence of bovine Out of the total 384 cattle examined, 335,
fasciolosis among different areas of the 21 and 28 were Indigenous, Holstein and
study may depend on some factors such Cross-breed cattle, respectively (Table 2).
as, snail population, choice of diagnostic The prevalence of fasciolosis was 33.1%,
method, livestock management system and 61.9% and 35.7% for Indigenous, Holstein
suitability of the environment for survival and Cross breed cattle, respectively. There
and distribution of the parasite as well as was statistically significant ( 2 = 7.135, P
the intermediate host might have played = 0.028) association of fasciolosis with
their own role in such breeds.
differences (Thomas, 1883; Yildirim et al.,
2007; Shiferaw et al., 2011) Regarding the effects of breed as showed
in Table 2, the infection was breed
As the shown in Table 1, from the total of dependent ( 2=7.135, P = 0.028) and the
152 male and 232 female cattle examined, highest infection rate was observed in
63(41.4%) and 71(30.6%) were positive Holstein breed (61.9%) followed by Cross
for fasciolosis, respectively. Analysis breed (35.7%) and Indigenous breed
indicated that, there was no significant (33.1%). This was in agreement with
association ( 2=0.688, p=0.407) between literature, which states that Holstein breed
prevalence of fasciolosis and sex of study are most affected than cross-breed and
animals. The result of the study (Table 1) Indigenous cattle breed were less likely to
indicated that, younger cattle (37.5%) be affected than both breeds (Castelino
were more affected as compared to adults and Preston, 1979; Mufti, 2011). This
(33.3%). Statistical analysis of the data could be explained by the fact that, the
showed that the presence of significant higher prevalence rate in Holstein breed
association ( 2 =4.573, P = 0.029) of and followed by cross-breed may be due to
Fasciola infections with age group. The the less adaptively capacity with the
present finding was in line with previous environment and Indigenous breeds are
studies by Shiferaw et al. (2011) around acquired a high degree of immunity as a
Assela, Mufti (2011) from Pakistan region, result of repeated natural exposure to
Kiyyu et al. (2003) from southern parasitic infections (Cohen and Watten,
highlands of Tanzania, Nganga et al. 1967). Out of 77, 87,119 and 101 cattle
(2004) from area of Kenya, who reported examined in Ambicho, Belessa, Shecha
that there was an age difference in the and Lereba Peasant Associations,
prevalence of fasciolosis. The possible 28(36.4%), 26(29.9%), 54(45.4%) and
explanation might be younger cattle were lastly 26 (25.7%) were found to be
more susceptible and less resistant to positive for fasciolosis, respectively. There
infection of fasciolosis than adults. Hence, was statistically significant ( 2 =10.515,
young animals with weak and less P=0.015) association in prevalence of
developed immunity were more likely to fasciolosis among different study location
be affected by fasciolosis than older (farms) of cattle examined (Table 2). The
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highest prevalence of fasciolosis was (1995) from Dire Dawa, 14% by Fufa et
recorded in Shecha (45.4%) peasant al. (2010) from Soddo, 20.3% by Kassaye
associations followed, in descending et al. (2012) from Addis Ababa abattoir,
order, by Ambicho (36.4%), Belessa and found to be lower than the prevalence
(29.9%) and Lereba (25.7%). Statistical of 46.58% by Tadela and Worku (2007)
analysis of the prevalence among peasant from Jimma and 54.5% by Abie et al.
associations indicated that, there was a (2012) from Jimma slaughter houses. The
significant association (p< 0.05) of variation of prevalence rate in different
infection rate of disease with animal study areas were probably due to the
origin. Accordingly, the occurrence of ecological and climatic difference between
bovine fasciolosis in Shecha Peasant the localities and the characters of soils
Association was the highest when that is important for multiplication of snail
compared to Ambicho, Belessa and hosts (Abie et al., 2012).
Lereba. Shecha has more appropriate
environmental conditions for the As shown in Table 3, from the total of 377
occurrence of intermediate hosts including males and 7 females, 114 (30.2%) and 3
flooded natural pastures, watershed areas, (42.8%), respectively were positive for of
slowly flowing waterways and rivers for fasciolosis. Among 24 young and 360
the presence of relatively more infection adult cattle examined during study period,
rate of fasciolosis in cattle. The finding of 14 (58.3%) and 103 (28.6%), respectively
the present study was in agreement with were positive for fasciolosis. Out of 343
the earlier findings by Torgerson (1999), Indigenous, 30 Holstein and 11Cross-
who reported that the interaction of breed cattle examined, 97(28.3%), 15
various environmental factors that increase (50%) and 5 (45.5%), respectively were
the likelihood of fasciolosis in cattle and positive for Fasciola species infection.
causes variation of infection rate between This study revealed that there was no
the localities. significant difference ( 2=0.517, P =0.440)
in fasciolosis infection on sex basis (Table
Prevalence of Fasciola Species Infection 5). This finding was in agreement with the
in Cattle earlier records reported by Opara (2005).
The number of slaughtered males in the
From the total of 384 cattle slaughtered at abattoir was higher than females. Even if
Hossana municipal abattoir, 117 (30.5%) the number of female cattle that come to
were found positive for fasciolosis on abattoir were fewer in number as
postmortem examination. This study was compared to males, the number of positive
comparable with the previous findings of females was higher in proportion than
30.43% by Hailu (1995) from Hawassa, males, hence the sample size matters in
29.75% by Rahel (2009) from Alaba, this case.
31.5% by Wakuma (2009) from Bedele,
32.3% by Mihreteab et al. (2010) from As Table 3 shown, young cattle (58.3%)
Adwa, 28.63% by Rahmeto et al. (2010) have significantly higher ( 2= 9.382,
from Hawassa and 29.75% by Mulat et al. P=0.002) prevalence rate of
(2012) from Gondar abattoirs. The finding fasciolosis when compared to adult ones (
of current study (30.5%) was relatively 28.6%).This may be attributed to the fact
higher when compared with the 14.4% that the immunity against disease increases
overall prevalence reported by Daniel with age (Dwinger, 1982; Yilma and
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Mesfin, 2000; Shiferaw et al., 2011; severely affected livers. The result of this
Mufti, 2011) or it could also be (most study was in agreement with the works by
probable) because of few number of young Dwinger et al. (1982) from Argentina,
animals presented for postmortem Mihreteab et al. (2010) from Adwa,
examination. The prevalence of fasciolosis Rahmeto et al. (2010) from Hawassa,
in Holstein breed were significantly Dechasa et al. (2012) from Jimma
(p<0.05) higher in susceptibility than municipal abattoirs. The mean fluke
Cross-breed. Indigenous breeds are less burden in moderately affected livers was
vulnerable than both breeds (Table 5). The higher than severely affected livers. This
result of this study was in agreement with may be attributed to the fact that, the bile
the findings of Kabir et al. (2010) from ducts of severely affected livers become
Bangladesh. Higher infection rate of calcified, fibrosed and having acquired
fasciolosis was recorded in holistein breed resistance and that leads to block the
cattle in this finding might be due to its further passage of undefined immature
few number as compare to cross or flukes in severely affected livers (Ramato,
indigenous breeds that came to 1992; Yilma and Mesfin, 2000; Dechasa et
postmortem examination. Hence, the al., 2012).
sample size matters.
Direct economic loss
Association of Severity of Liver
Pathological Lesion with Fluke Burden A direct economic loss as a result of
in Cattle fasciolosis in cattle was estimated on liver
condemnation at Hossana Municipal
There was a statistically significant abattoir. The calculation on direct
variation (F =171.47, P=0.000) of mean economic loss of bovine fasciolosis was
fluke count between the levels of severity based on the average number of cattle
of liver lesions (Table 9). The average slaughtered per year, mean selling price of
mean fluke count encountered during the the cattle livers at Hossana town and the
study was 27.48 flukes per affected liver prevalence of fasciolosis in the present
and it was lower as compared to the report study (30.5%). The average market price
by Dechasa et al. (2012), who reported 50 of 1Kg of meat and one liver at Hossana
flukes per affected liver. The possible town was taken as 120 and 55 Ethiopian
reason of being less in average mean birr, respectively. The mean number of
flukes count may be due to the lack of cattle slaughtered in this municipal
rainfall and moisture at a point of study abattoir was 5294 per year depends on two
period for the replication of snails as well years recorded data.
as Fasciola species (Brown, 2005).
The direct economic loss was calculated
according to the mathematical formula
The mean fluke burden in lightly, derived by Ogunrinade and Ogunrinade
moderately and severely affected livers (1980) as follows:
was found to be
ALC = MCS x MLC x P
7.00±0.513, 34.37±4.369 and
26.76±2.712, respectively. It was higher in Where ALC=Annual loss from Liver
moderately affected livers and followed by
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Table.1 Prevalence of fasciolosis by sex and age based on coprological examination of cattle
owned by smallholder farmers in Lemo district, southern Ethiopia from February-April, 2012
Table.2 Prevalence of Fasciola species infection by breed of cattle and study farms in Lemo
district, southern Ethiopia from February-April, 2012
Variables
2
examined Positive (%) 95% CI OR p-value
Breed Indigenous 335 111(33.1%) 28.1-38.5
Holstein 21 13 (61.9%) 38.4-81.9
Cross breed 28 10 (35.7%) 18.6-55.9
Total 384 134 (34.9%) 30.1-39.9 1.247 7.135 0.028
Location Ambicho 77 28 (36.4%) 25.7-48.1
Belessa 87 26 (29.9%) 20.5-40.6
Shecha 119 54 (45.4%) 36.2-54.8
Lereba 101 26 (25.7%) 17.6-35.4
Total 384 134 (34.9%) 30.1-39.9 1.023 10.515 0.015
Table.3 Prevalence of fasciolosis by sex, age and breed of cattle slaughtered at Hossana
municipal abattoir, southern Ethiopia from February-April, 2012
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