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4. Judul Tugas : Analisis Kadar Asam Salisilat Dalam Bedak Dengan Metode
Spektrofotometri Visible
5. Bentuk Sediaan : Serbuk
6. Pustaka Acuan :
Higuchi Takeru, Einar Brochmann Hanssen, 1961, Pharmaceutical
Analysis Intercience Publisher, New York, p. 22-23
Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia
nomor 23 tahun 2019 Tentang Persyaratan Teknis Bahan Kosmetika
7. Acuan yang dipilih :
Higuchi Takeru, Einar Brochmann Hanssen, 1961, Pharmaceutical Analysis
Intercience Publisher, New York, p. 22-23
I. Prosedur Asli
Procedure
A sample, corresponding to upproximately 0.2 g. of salicylic acid, is
weighed accurately and dissolved in 15 ml. of alcohol. This solution is made up to
a volume of exactly 1 liter with water and is filtered if necessary rejecting the first
50 ml. of filtrate. An aliquot of the clear solution of 25 ml. is placed in a 100 ml.
volumetric fask and 5 ml. of the ferric nitrate solution is added. The solution is
made up to volume and read on the spectrophotometer as in the case of the
calibration curve. The concentration of the solution is then read from the
calibration curve. If the absorbance of the solution does not fall betwen 0.1 and
0.7, it i suggested that another aliquol, of such a size that the reading will fall
within these limits, be taken and that the calculation be adjusted to account
for the difference.
Alternatively, the A1c 1% at 525 mµ may be determined in place of
m
preparing a calibration curve, by dividing the absorbance by the present
concentration of a solution of known concentration. The absorbance values
obtained as described for the calibration curve may be used, and an average
value for the absorptively calculated. The percent concentration of the dilution
being determined is then obtained by dividing the absorbance read for
that .
1%
dilution by the 1cm
A
Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia nomor 23 tahun 2019
Tentang Persyaratan Teknis Bahan Kosmetika
Alat :
Instrumen Spektrofotometer UV-Vis
Botol Timbang (1)
Labu Ukur 100,0 ml , 50,0 ml (4/4)
Pipet volume 1,0 ml ; 2,0 ml ; 3,0 ml ; 5,0 ml ; 10,0 ml (1/1/1/1/1)
Pengaduk kaca (1)
Timbangan analitik
Timbangan milligram
Anak timbangan
Kertas timbang
Corong Kaca (2)
Kertas Saring
Kuvet (1)
Mortir dan stamper (1/1)
Sudip (1)
Kertas lensa
2. Buatlah data rentang baku kerja, simulasi bau kerja (bpj) vs absorbansi berdasarkan
A11 yang telah didapat dalam pustaka
Jawab :
Syarat absorbansi = A = 0,2 – 0,8
Spektrofotometer Visibel
525 nm =116
Perhitungan absorbansi =
- 10.000 bpj => A = 116
20 bpj => A = ...
A= 20 bpj / 10,000 bpj x 116 = 0,232
- 10.000 bpj => A = 116
30 bpj => A = ...
A= 30 bpj / 10,000 bpj x 116 = 0,348
- 10.000 bpj => A = 116
40 bpj => A = ...
A= 40 bpj / 10,000 bpj x 116 = 0,464
- 10.000 bpj => A = 116
50 bpj => A = ...
A= 50 bpj / 10,000 bpj x 116 = 0,58
- 10.000 bpj => A = 116
60 bpj => A = ...
A= 60 bpj / 10,000 bpj x 116 = 0,696
a. Sampel 1
Absorbansi = 0,782
A sampel
C sampel = x C baku kerja
A baku kerja
0,782
C sampel = x60
0,696
b. Sampel 2
Konsentrasi sampel = 1.980 mg/100,0 mL 19.800 bpj
Dipipet 10,0 mL ad 100,0 mL pengenceran 10x
A sampel
C sampel = x C baku kerja
A baku kerja
0,768
C sampel = x60
0,696
d. Sampel 4
Konsentrasi sampel = 1.850 mg/100,0 mL 18.500 bpj
Dipipet 10,0 mL ad 100,0 mL pengenceran 10x