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Dhafer M Aziz
University of Mosul
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A lack of appetite is
one of the earliest signs
of bitch might
be pregnant.
Signs of Canine Pregnancy
3. Breast Development
Breast development is a good
indicator for pregnancy changes.
The nipples of an unbred female
are usually small, and the area
beneath them feels flat.
4. Change in Nipple Color
Abdominal palpation
Radiography
Ultra sonography
Relaxin assay
Acute phase proteins
Abdominal palpation
Tradional method of in bitches and queens.
is accurate between 24- to 35-day post- breeding.
At about 30-35 days the accuracy is high 87%.
in the flank and also in the lower abdomen
Gestational sacs are palpated as tense conceptual
swellings (25 to 30 mm in diameter) within the uterine cornua
These swellings double in size every week until days 35 to 38.
Disadvantages of Abdominal palpation
Disadvantages
High cost of machine
Not the method of choice for assessment of litter size
Fetal position is difficult to determine
Trained person is required to perform USG
Radiography
Radiography is done after 45 days of mating
100% accurate in last 15 days of pregnancy
Mineralization of bones starts 44 day onwards
Lateral sides are mineralized earlier than ventro dorsal
projections
Radiography in lateral recumbency is preferred than dorsal or
dorso ventral
voltage should be 50 Kvp
current should be 0.5 – 1 mA
Advantages
Good in evaluating fetal numbers.
Fetus can be differentiated easily from abdominal
contents due to mineralization of bones after 45 days.
Position of the fetuses can be easily determined.
Number of fetuses can be easily counted.
Whelping date can be predicted
Disadvantages
Poor in evaluating fetal viability.
High cost of machine
Hazards of exposure to X Rays to growing fetuses &
operator
Hormonal assay
Relaxin
Pregnancy-specific hormone in dogs
Secreted by placenta only
Differential hormone between pregnancy &
pseudo pregnancy
Peak conc. Between 20–30 days of gestation
Relaxin assay cannot be used to estimate litter size
Acute phase proteins
Increase after day 20 of gestation
Released during pregnancy & inflammatory diseases
Include haptoglobin, ceruloplasmin, alpha-globulin,
C-reactive protein and fibrinogen.
Serum fibrinogen as a pregnancy confirmatory test is;
98% accurate, with a value >280 mg/dL
Nearly 100% accurate with a value of>300 mg/ dL
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