University of Bahrain

College of Engineering
Department of Instrumentation and Control Engineering

Fluorescence spectroscopy

pg. 1
 Table of Contents
1. Aim.......................................................................................................3
2. Introduction..........................................................................................3
3. Equipment And Chemicals...................................................................4
4. Calculations.........................................................................................4
4.1 Raw Data...........................................................................................4
4.2 Substituting.....................................................................................5
5. Conclusion...........................................................................................5
6. References...........................................................................................5

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1. Aim
Determination of anthracene concentration in an unknown sample .

2. Introduction
Fluorescence spectroscopy is a type of
electromagnetic spectroscopy that analyzes
fluorescence from a sample. Fluorescence is a
type of luminescence caused by photons exciting
a molecule, raising it to an electronic excited
state.

Fluorescence is analytically important emission

processes in which atoms or molecules are
excited by the absorption of a beam of
electromagnetic radiation. The excited species
then relax to the ground state, giving up their
excess energy as photons. Fluorescence takes
place much more rapidly
than phosphorescence .

Fluorescence is a photoluminescence process in which atoms or molecules are

excited by absorption of electromagnetic radiation, The excited species then relax
back to the ground state, giving up their excess energy as photons, the lifetime of
an excited species is brief because there are several mechanisms for an excited
atom or molecule to give up its excess energy and relax to its ground state. Two of
the most important of these mechanisms, nonradiative relaxation and fluorescence
emission.

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3. Equipment And Chemicals

 Fluorimeter
(florescence
spectroscopy
instrument) .
 Unknowns
Samples .

4. Calculations

4.1 Raw Data

Standard solution Graph

700
Concentratio
Intensity of Fluorescence (401.15 nm)

n of
600 f(x) = 477.37 x + 167.24
Intensity of
Anthracene
Fluorescence
Standard
500
(401.15 nm)
Solution
400
(ppm)
0.2
300 230.61
200
0.4 355.06
100
0.6 515.07
0
0.8 0.1 0.2 564.12
0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1
Concentration of Anthracene Standard Solution (ppm)
1 603.45

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 Unknowns samples

Sample 4 Sample 7
intensity 450.05 300.55
3.2 Substituting

sample 4:
Intensity (I) =450.05
by straight line equation : I = 477.37C + 167.24

450.05 = 477.37 x (C) + 167.24

Concentration (C) =0.5924 ppm
sample 7: Intensity (I) =300.55
by straight line equation : I = 477.37C + 167.24

300.55 = 477.37 x (C) + 167.24

Concentration (C) =0.2793 ppm

5. Conclusion
In this experiment, we used the Fluorescence Emission Spectrometry method
experiment to measure the concentration of anthracene .we used linear equation
which is showing a linear relationship between intensity and concentration .by
plotting the results ,the relation be shown easily.
We can summary the experiment in few sentences,
• Fluorescence spectrometry is a fast, simple and inexpensive method to determine
the concentration of an analyte in solution based on its fluorescent properties.
• Fluorescence spectroscopy is used in biochemical, medical, and chemical
research fields for analysing organic compounds .

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6. References
1- Ppt slides , Mr. Sadiq Maayoof , Chemistry Department , University of
Bahrain
2- fundamentals of analytical chemistry 9E, Douglas A. Skoog , Donald M.
West, F.James Holler, Stanley R. Crouch .
3- YouTube video, Zeal’s pharmacy tutorials channel, Fluorescence
spectroscopy.

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