3.

Corrected excitation spectra

CORRECTED EXCITATION SPECTRA
CONCENTRATION (ppm) INTENSITY
0 0
0.02 85.39
0.04 172.13
0.06 264.33
0.08 344.53
0.10 441.95
Sample 1 (0.0334) 147.00
Sample 2 (0.0318) 138.19
Sample 3 (0.0338) 139.45

CORRECTED EXCITATION SPECTRA:

INTENSITY VS CONCENTRATION
500.00
441.95
450.00
400.00 f(x) = 4399.1 x − 1.9
R² = 1 344.53
350.00
300.00 264.33
INTENSITY

250.00
200.00 172.13
150.00
85.39
100.00
50.00
0.00
0.00
0.00 0.02 0.04 0.06 0.08 0.10 0.12
CONCENTRATION (ppm)

From the graph:

Sample 1:

y = 4399.1x – 1.9
145.13 = 4399.1x – 1.9

4399.1x = 147.03

x = 0.0334

Sample 2:

y = 4399.1x – 1.9

138.19 = 4399.1x – 1.9

4399.1x = 140.09

x = 0.0318

Sample 3:

y = 4399.1x – 1.9

146.85 = 4399.1x – 1.9

4399.1x = 148.75

x = 0.0338

ΣN
Mean =
N

0.0334+0.0318+ 0.0338
Mean = = 0.033 ppm
3

Actual concentration of sample = mean calculated concentration x dilution factor

= 0.033 ppm x 250

= 8.25 ppm

2
Standard Deviation σ= √∑ ( x− x́ )
n−1

σ = √ [(0.0334 – 0.033)2 + (0.0318 – 0.033)2 + (0.0338 – 0.033)2] / 3 - 1 = 1.0583 x 10-3

Standard Deviation
Relative Standard Deviation, RSD = x 100%
Mean

1.0583 x 10−3
= x 100%
0.033
 = 0.0321

Amount of Riboflavin in energy drink = 8.25 mg/ L x 0.05 L

= 0.4125 mg = 4.125 x 10-4 g

Energy Drink Sample Volume = 250 ml

4.125 x 10−4 g
Percent Weight per Volume of Riboflavin = x 100 %
250 ml

= 1.65x10-4% (w/v)

0.4125 mg
Concentration of Riboflavin from Energy Drinks = = 1.65 mg/L (ppm)
0.25 L