Scribd Logo
Upload

Welcome to Scribd!

  • Data Pengamatan 1. Perhitungan Larutan Induk

    Uploaded by

    Farah Yumna Ambaro
    8 views4 pages
    1. The document details the preparation of stock solutions of different concentrations from a 1000 ppm parent solution to create a calibration curve. 2. Absorbance readings were taken over time for samples and used to calculate flux values through a diffusion membrane. 3. The maximum flux value was determined to be 0.0424 mg/cm2 at 60 minutes, and the percentage of drug diffused over 120 minutes was calculated to be 5.325% of the initial dose.

    Original Description:

    Data pengamatan praktikum

    Original Title

    datpeng modul 5

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    1. The document details the preparation of stock solutions of different concentrations from a 1000 ppm parent solution to create a calibration curve. 2. Absorbance readings were taken over time for samples and used to calculate flux values through a diffusion membrane. 3. The maximum flux value was determined to be 0.0424 mg/cm2 at 60 minutes, and the percentage of drug diffused over 120 minutes was calculated to be 5.325% of the initial dose.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    8 views4 pages

    Data Pengamatan 1. Perhitungan Larutan Induk

    Uploaded by

    Farah Yumna Ambaro
    1. The document details the preparation of stock solutions of different concentrations from a 1000 ppm parent solution to create a calibration curve. 2. Absorbance readings were taken over time for samples and used to calculate flux values through a diffusion membrane. 3. The maximum flux value was determined to be 0.0424 mg/cm2 at 60 minutes, and the percentage of drug diffused over 120 minutes was calculated to be 5.325% of the initial dose.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 4

    Data Pengamatan

    1. Perhitungan Larutan Induk


    Larutan induk yang dibuat adalah 1000 ppm dalam 10 mL, maka zat aktif
    yang ditimbang adalah 1000 ppm = 1000 μg/mL
    1000 μg
    10 mL → x 10 mL = 10000 μg = 10 mg
    1 mL

    2. Perhitungan Larutan Stok


    7,5 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 7,5 ppm
    V1 = 0,075 ppm
    10 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 10 ppm
    V1 = 0,1 ppm
    12,5 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 12,5 ppm
    V1 = 0,125 ppm
    15 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 15 ppm
    V1 = 0,15 ppm
    17,5 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 17,5 ppm
    V1 = 0,175 ppm
    20 ppm → V1 . N1 = V2 . N2
    V1 . 1000 ppm = 10 mL . 20 ppm
    V1 = 0,2 ppm

    3. Data Kurva Kalibrasi


    Konsentrasi (ppm) Absorbansi (A)
    7,5 0,331
    10 0,451
    12,5 0,6
    15 0,718
    17,5 0,749
    20 0,834

    Tabel 1. Data Kurva Kalibrasi


    a = 0.064 b = 0,040 r = 0,978
    y = 0,040x + 0,064

    4. Data Absorbansi Sampel


    Waktu (menit) Absorbansi (A)
    15 0,356
    30 0,378
    60 0,338
    90 0,214
    120 0,134
    Tabel 2. Data Absorbansi Sampel

    5. Perhitungan Sampel Uji


    y = 0,040x + 0,064
    t15 → 0,356 = 0,040 x + 0,064
    x0,356−0,064
    =
    0,040
    x = 7,3 x 15 = 109,5 μg
    t30 → 0,378 = 0,040 x + 0,064
    x0,378−0,064
    =
    0,040
    x = 7,85 x 15 = 117,75 μg
    t60 → 0,338 = 0,040 x + 0,064
    x0,338−0,064
    =
    0,040
    x = 6,85 x 15 = 102,75 μg
    t90 → 0,214 = 0,040 x + 0,064
    x0,214−0,064
    =
    0,040
    x = 3,75 x 15 = 56,25 μg
    t120 → 0,134 = 0,040 x + 0,064
    x0,134−0,064
    =
    0,040
    x = 1,75 x 15 = 26,25 μg

    6. Perhitungan Faktor Koreksi


    C15 = 109,5 μg = 0,109 mg
    C30 2 mL
    = 117,75 + ( x 109,5)
    5 mL
    =
    117,75 + 14,6 = 132, 35 μg = 0,132 mg
    C60 2 mL
    = 102,75 + [ x (109,5+117,75)]
    5 mL
    =
    102,75 + 30,3 = 133, 05 μg = 0,133 mg
    C90 2 mL
    = 56,25 + [ x (109,5+117,75+102,75)]
    5 mL
    =
    56,25 + 44 = 100, 25 μg = 0,1 mg
    C120 2 mL
    = 26,25 + [ x (109,5+117,75+102,75+56,25)]
    5 mL
    =
    26,25 + 51,5 = 77, 75 μg = 0,078 mg

    7. Nilai Fluks (J)


    Diketahui: D = 2 cm
    Jt15 0,109 mg
    = = 0,035 mg/cm2
    3,14 cm²
    Jt30 0,132mg
    = = 0,0420 mg/cm2
    3,14 cm²
    Jt60 0,133 mg
    = = 0,0424 mg/cm2
    3,14 cm²
    Jt90 0,1 mg
    = = 0,032 mg/cm2
    3,14 cm ²
    Jt120 0,078 mg
    = = 0,025 mg/cm2
    3,14 cm²

    8. Perhitungan % Difusi
    Dosis = 2,5 mg
    J tertinggi
    % dosis = dosis x 100%
    luas lingkaran
    0,0424 mg/cm ²
    = x 100%
    2,5 mg/3,14 cm²
    = 5,325 %

    0.05

    0.04

    0.04

    0.03

    0.03

    0.02

    0.02

    0.01

    0.01

    0
    15 30 60 90 120

    Grafik 1. Grafik Difusi Piroksikam (Waktu vs Nilai Fluks)

    You might also like