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  • Konsentrasi (PPM) Absorbansi

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    Selvy Jumiatul
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    1. The document describes calculations to determine the concentration of gallic acid in samples. It measures the absorbance of gallic acid solutions at different concentrations and extracts of shallot bulbs at various ratios to determine the gallic acid content. 2. Extracts of shallot bulbs at ratios of 2:1, 4:1, 5:1, and 6:1 to solvent were tested in triplicate. Absorbance readings were used to calculate the gallic acid concentration through linear regression analysis. 3. Gallic acid content was highest at around 60-63 mgGAE/g for 2:1 ratio, 9-12 mgGAE/g for 4:1, 13-15

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    1. The document describes calculations to determine the concentration of gallic acid in samples. It measures the absorbance of gallic acid solutions at different concentrations and extracts of shallot bulbs at various ratios to determine the gallic acid content. 2. Extracts of shallot bulbs at ratios of 2:1, 4:1, 5:1, and 6:1 to solvent were tested in triplicate. Absorbance readings were used to calculate the gallic acid concentration through linear regression analysis. 3. Gallic acid content was highest at around 60-63 mgGAE/g for 2:1 ratio, 9-12 mgGAE/g for 4:1, 13-15

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    9 views3 pages

    Konsentrasi (PPM) Absorbansi

    Uploaded by

    Selvy Jumiatul
    1. The document describes calculations to determine the concentration of gallic acid in samples. It measures the absorbance of gallic acid solutions at different concentrations and extracts of shallot bulbs at various ratios to determine the gallic acid content. 2. Extracts of shallot bulbs at ratios of 2:1, 4:1, 5:1, and 6:1 to solvent were tested in triplicate. Absorbance readings were used to calculate the gallic acid concentration through linear regression analysis. 3. Gallic acid content was highest at around 60-63 mgGAE/g for 2:1 ratio, 9-12 mgGAE/g for 4:1, 13-15

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    © All Rights Reserved
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    of 3

    Perhitungan

    1. Asam Galat

    Konsentrasi (ppm) Absorbansi


    12,5 0,047
    25 0,049
    50 0,089
    100 0,162
    200 0,332
    a = 0,015
    b = 0,001559
    r = 0,997

    2. Ekstrak Bawang Dayak


    a. Rasio 2:1
    Absorbansi ekstrak : 0,192;0,193;0,192
    Absorbansi pelarut : 0,060;0,064;0,064
    10−2
    Pengenceran : x 5 = 0,0012
    42

    i. y = a + bx
    0,132 = 0,015 + 0,001559x
    x = 75,05
    x = 0,075 mgGAE
    0,075
    => = 62,5 mgGAE/g sampel
    0,0012
    ii. y = a + bx
    0,129 = 0,015 + 0,001559x
    x = 73,12
    x = 0,073 mgGAE
    0,07 3
    => = 60,8 mgGAE/g sampel
    0,0012
    iii. y = a + bx
    0,132 = 0,015 + 0,001559x
    x = 72,48
    x = 0,072 mgGAE
    0,072
    => = 60 mgGAE/g sampel
    0,0012

    b. Rasio 4:1
    Absorbansi ekstrak : 0,097;0,097;0,100
    Absorbansi pelarut : 0,065;0,062;0,063
    10−2
    Pengenceran : x 5 = 0,0012
    41,5

    i. y = a + bx
    0,032 = 0,015 + 0,001559x
    x = 10,9
    x = 0,011 mgGAE
    0,011
    => = 9,17 mgGAE/g sampel
    0,0012
    ii. y = a + bx
    0,035 = 0,015 + 0,001559x
    x = 12,8
    x = 0,013 mgGAE
    0,013
    => = 10,8 mgGAE/g sampel
    0,0012
    iii. y = a + bx
    0,037 = 0,015 + 0,001559x
    x = 14,11
    x = 0,014 mgGAE
    0,014
    => = 11,7 mgGAE/g sampel
    0,0012

    c. Rasio 5:1
    Absorbansi ekstrak : 0,103;0,108;0,106
    Absorbansi pelarut : 0,061;0,063;0,064
    10−2
    Pengenceran : x 5 = 0,00125
    40

    i. y = a + bx
    0,042 = 0,015 + 0,001559x
    x = 17,32
    x = 0,017 mgGAE
    0,017
    => = 13,6 mgGAE/g sampel
    0,00125
    ii. y = a + bx
    0,045 = 0,015 + 0,001559x
    x = 19,24
    x = 0,019 mgGAE
    0,019
    => = 15,2 mgGAE/g sampel
    0,00125
    iii. y = a + bx
    0,042 = 0,015 + 0,001559x
    x = 17,32
    x = 0,017 mgGAE
    0,017
    => = 13,6 mgGAE/g sampel
    0,00125
    d. Rasio 6:1
    Absorbansi ekstrak : 0,104;0,107;0,108
    Absorbansi pelarut : 0,066;0,065;0,065
    10−2
    Pengenceran : x 5 = 0,00116
    43

    i. y = a + bx
    0,038 = 0,015 + 0,001559x
    x = 14,75
    x = 0,015 mgGAE
    0,015
    => = 12,9 mgGAE/g sampel
    0,00116
    ii. y = a + bx
    0,042 = 0,015 + 0,001559x
    x = 17,3
    x = 0,017 mgGAE
    0,017
    => = 14,65 mgGAE/g sampel
    0,00116
    iii. y = a + bx
    0,043 = 0,015 + 0,001559x
    x = 17,9
    x = 0,018 mgGAE
    0,018
    => = 15,5 mgGAE/g sampel
    0,00116

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