DATA PRAKTIKUM SPEKTRO MULTIVARIAT

PCT DAN GG

 PARACETAMOL
ƛ Paracetamol = 245nm
absorbtivitas = 668a
Range absorbansi = 0,2-0,8
Batas bawah Batas atas
A =axbxc A =axbxc
0,2 = 668 x 1 x c 0,8 = 668 x 1 x c
C = 0,2/668 C = 0,8/668
C = 0,000299 x 104 C = 0,00119 x 104
C = 2,99 ppm C = 11,9 ppm

Larutan induk Paracetamol = 100 ppm 10 mg Paracetamol ad 100 ml Aqua acid

5 seri konsentrasi larutan Paracetamol

Ppm Pembuatan
3 ppm 3 ml ad 100 ml aqua acid
4 ppm 1 ml ad 25 ml aqua acid
6 ppm 3 ml ad 50 ml aqua acid
8 ppm 2 ml ad 25 ml aqua acid
10 ppm 5 ml ad 50 ml aqua acid

 GG
ƛ GG = 273nm
absorbtivitas = 125a
Range absorbansi = 0,2-0,8
Batas bawah Batas atas
A =axbxc A =axbxc
0,2 = 125 x 1 x c 0,8 = 125 x 1 x c
C = 0,2/125 C = 0,8/125
C = 0,0016 x 104 C = 0,0064 x 104
C = 16 ppm C = 64 ppm

Larutan induk GG = 100 ppm 10 mg GG ad 100 ml Aqua acid

5 seri konsentrasi larutan GG

Ppm Pembuatan
16 ppm 4 ml ad 25 ml aqua acid
20 ppm 5 ml ad 25 ml aqua acid
30 ppm 3 ml ad 10 ml aqua acid
40 ppm 10 ml ad 25 ml aqua acid
 50 ppm 5 ml ad 10 ml aqua acid

 DATA ABSORBANSI

PCTƛPCT
ppm Absorbansi
3 0,112
4 0,237
6 0,351
8 0,46
10 0,61
K11 = 0,067

PCTƛGG
ppm Absorbansi
3 0,036
4 0,06
6 0,1
8 0,115
10 0,149
K21 = 0,0154

GGƛGG
ppm Absorbansi
16 0,158
20 0,223
30 0,325
40 0,459
50 0,627
K22 = 0,0134

GGƛPCT
ppm Absorbansi
16 0,041
20 0,032
30 0,055
40 0,077
50 0,164
K12 = 0,0034

 PREPRARASI SAMPEL
Sampel di larutkan aqua acid ad 100 ml, diambil 1 ml di ad kan aqua acid 50 ml dan
diambil 1 ml di ad kan aqua acid 100 ml.
Absorbansi Sampel ad 50 ml di ƛ PCT = 0,301; 0,301; 0,301 = 0,301
 Absorbansi Sampel ad 100 ml di ƛ PCT = 0,286; 0,286; 0,286 = 0,286
Absorbansi Sampel ad 50 ml di ƛ GG = 0,162; 0,163; 0,163 = 0,163
Absorbansi Sampel ad 100 ml di ƛ GG = 0,083; 0,093; 0,093 = 0,093

 PERHITUNGAN KADAR
 SAMPEL 50 ML
A1 = K11.C1 + K12.C2
A2 = K21.C1 + K22.C2
0,301 = 0,067.C1 + 0,0034.C2
0,163 = 0,0154.C1 + 0,0134.C2
ELIMINASI
0,301 = 0,067.C1 + 0,0034.C2 x 0,0134
0,163 = 0,0154.C1 + 0,0134.C2 x 0,0034
0,0040334 = 0,0008978.C1 + 0,00004556.C2
0,0005542 = 0,00005236.C1 + 0,00004556.C2 -
0,0034792 = 0,00084544.C1
C1 = 4,11 ppm

SUBSTITUSI
A1 = K11.C1 + K12.C2
0,301 = 0,067.C1 + 0,0034.C2
0,301 = 0,067. 4,11+ 0,0034.C2
0,301 = 0,27537 + 0,0034.C2
0,0034.C2 = 0,301 – 0,27537
0,0034.C2 = 0,02563
C2 = 7,54 ppm

 Ppm sampel : 150 mg : 100 ml = 1,5 x 1000 = 1500 ppm

 Pengenceran : V1.C1 = V2.C2
1.1500 = 50.C2
C2 = 30 ppm
 Kadar Paracetamol = (4,11 ppm : 30 ppm) x 100% = 13,7%
= 13,7% x 150 mg = 20,55 mg
 Kadar GG = (7,54 ppm : 30 ppm) x 100% = 25,13%
= 25,13% x 150 mg = 37,695 mg

 SAMPEL 100 ML
A1 = K11.C1 + K12.C2
A2 = K21.C1 + K22.C2
0,286 = 0,067.C1 + 0,0034.C2
0,093 = 0,0154.C1 + 0,0134.C2
ELIMINASI
0,286 = 0,067.C1 + 0,0034.C2 x 0,0134
 0,093 = 0,0154.C1 + 0,0134.C2 x 0,0034

0,0038324 = 0,0008978.C1 + 0,00004556.C2

0,0003162 = 0,00005236.C1 + 0,00004556.C2 -
0,0035162 = 0,00084544.C1
C1 = 4,16 ppm

SUBSTITUSI
A1 = K11.C1 + K12.C2
0,286 = 0,067.C1 + 0,0034.C2
0,286 = 0,067. 4,16 + 0,0034.C2
0,286 = 0,27872 + 0,0034.C2
0,0034.C2 = 0,286 – 0,27872
0,0034.C2 = 0,00728
C2 = 2,14 ppm

 Ppm sampel : 150 mg : 100 ml = 1,5 x 1000 = 1500 ppm

 Pengenceran : V1.C1 = V2.C2
1.1500 = 100.C2
C2 = 15 ppm
 Kadar Paracetamol = (4,16 ppm : 15 ppm) x 100% = 27,73%
= 27,73% x 150 mg = 41,595 mg
 Kadar GG = (2,14 ppm : 15 ppm) x 100% = 14,27%
= 14,27% x 150 mg = 21,405 mg