Data Pengamatan

5.1. Perhitungan pembuatan dapar fosfat pH 7,4 sebanyak 1000 ml

KH₂PO₄ = 0,2 M (BM = 136 g/mol)

NaOH = 0,2 M (BM = 40 g/mol)
 Dibuat dalam 1000 ml
gr 1000 ml
 KH₂PO₄ → M = X
BM V
gr 1000 ml
X
0,2 = g 50 ml
136
ml
g = 1,36 gr
gr 1000 ml
 NaOH → M = X
BM V
g
0 ,2 M x 40 x 39 , 1 ml
gr = ml = 0,31 gr
1000 ml

 Untuk 1000 ml perlu dilakukan penimbangan sebanyak:

1000 ml 1000 ml
KH₂PO₄ = x 1 ,3 6 gr NaOH = x 0 , 31 gr
200 ml 200 ml
¿ 6 , 8 gr ¿ 1 ,5 5 gr
Aquadest:
1000 ml NaOH
KH₂PO₄ = x 500 ml
200 ml 1000 ml
= x 39 ,1 ml=19 6 ml
¿ 250 ml 200 ml

5.2. Perhitungan larutan standar piroksikam 100 ppm dalam 100 ml

μg
Ppm =
ml
100 μ g
Ppm = x 100 ml=10.000 μg=10 mg
ml
5.3. Pembuatan kurva kalibrasi piroksikam

Konsentrasi (ppm) Absorbansi

5 0,223
7 0,301
9 0,395
11 0,494
13 0,577
15 0,655

 Persamaan regresi linier:

a= -0,0002
b= 0,0441
r= 0,9993
y = bx ± a
y = 0,0441x – 0,0002

 Perhitungan Pengenceran
 5 ppm  13 ppm
V1 x 100 ppm = 10 ml x 5 ppm V1 x 100 ppm = 10 ml x
V1 = 0,5 ml 13 ppm
 7 ppm V1 = 1,3 ml
V1 x 100 ppm = 10 ml x 7 ppm  15 ppm
V1 = 0,7 ml V1 x 100 ppm = 10 ml x
 9 ppm 15 ppm
V1 x 100 ppm = 10 ml x 9 ppm V1 = 1,5 ml
V1 = 0,9 ml
 11 ppm
V1 x 100 ppm = 10 ml x 11 ppm
V1 = 1,1 ml
 5.4. Perhitungan % difusi perbulan gel piroksikam

Waktu Absorbansi Konsentrasi Kadar Kadar Fluks/J(t)

(menit) (mcg/ml) kompartemen/Ct terkoreksi/Ct mg/cm2
(mcg) erkoreksi
5 0,110 2,4989 37,4835 mcg/ml 37,9835mcg 11,9374
mcg/cm2
15 0,191 4,3356 65,034 mcg/ml 70,0318 mcg 37,9835
mcg/cm2
30 0,326 7,3968 110,952 mcg/ml 125,287 mcg 39,9003
mcg/cm2
45 0,408 9,2562 139,843 mcg/ml 168,883 mcg 54,1029
mcg/cm2
60 0,494 11,2063 168,0945 mcg/ml 221,786 mcg 70,6325
mcg/cm2
90 0,653 14,8118 222,177 mcg/ml 305,440 mcg 97,2739
mcg/cm2

5.4.1. Perhitungan konsentrasi piroksikam

(y = 0,0041x - 0,002)

 C5 0,191 + 0,0002 = 0,0441x

0,110 = 0,0441x - 0,0002
0,110 + 0,0002 = 0,0441x
x = 2,4989 mg/ml
 C15
0,191 = 0,0441x - 0,0002
x = 4,3356 mg/ml
 C30
0,326 = 0,0441x - 0,0002
0,326 + 0,0002 = 0,0441x
 x = 7,3968 mg/ml 0,494 = 0,0441x - 0,0002
0,494 + 0,0002 = 0,0441x
 C45 x = 11,2063 mg/ml
0,408 = 0,0441x - 0,0002  C90
0,408 + 0,0002 = 0,0441x 0,653 = 0,0441x - 0,0002
x = 9,2562 mg/ml 0,653 + 0,0002 = 0,0441x
 C60 x = 14,8118 mg/ml

5.4.2. Perhitungan kadar piroksikam pada kompartemen reseptor

C(t) = konsentrasi ( mcg

ml )
x volume kompartemen (ml)

 C5 = 2,4989 mcg/ml x 15 ml = 37,4835 mcg

 C15 = 4,3356 mcg/ml x 15 ml = 65,034 mcg
 C30 = 7,3968 mcg/ml x 15 ml = 110,952 mcg
 C45 = 9,2562 mcg/ml x 15 ml = 138,843 mcg
 C60 = 11,2063 mcg/ml x 15 ml = 168,0945 mcg
 C90 = 14,8118 mcg/ml x 15 ml = 222,177 mcg

5.4.3. Perhitungan kadar terkoreksi piroksikam

Cterkoreksi (t) = C(t) + ( Volume sampling ( ml )

)
Volume kompartemen ( ml )
x Ʃ(terkoreksi sebelumnya)

 Ct 5 = 37,4835 mcg + ( 25 mlml ) x 0 ¿ = 37,9835 mcg

Ct 15 = 65,034 mcg + (
5 ml )
2 ml
 x 37,9835 mcg¿ = 70,0318 mcg

Ct 30 = 110,952 mcg + (
5 ml )
2 ml
 x 37,9835 mcg+ 70,0318 mcg¿ = 125,287 mcg
 Ct 45 = 138,843 mcg + ( 25 mlml ) x 37,9835 mcg+ 70,0318 mcg+ 125,287 mcg ¿ =
169,883 mcg
 Ct 60 = 168,0945 mcg +

( 25 mlml ) x 37,9835 mcg+ 70,0318 mcg+ 125,287 mcg+169,883 mcg¿ = 221,786

mcg
 Ct 90 = 259,55 mcg +

( 152 mlml ) x 37,9835 mcg+ 70,0318 mcg+ 125,287 mcg+169,883 mcg+221,786 mcg ¿
= 305,440 mcg

5.4.4. Perhitungan nilai fluks

Cterkoreksi(t)
J(t) =
Luas lingkaran(c m2)

37,4835 mcg
 J5 = = 11,9374 mcg/cm²
3 , 14 cm ²
70,0318 mcg
 J15 = = 22,3031 mcg/cm²
3 , 14 cm ²
125,287 mcg
 J30 = = 39,9003 mcg/cm²
3 , 14 cm ²
169,883 mcg
 J45 = = 54,1029 mcg/cm²
3 , 14 cm ²
221,7860 mcg
 J60 = = 70,63 mcg/cm²
3 , 14 cm ²
305,440 mcg
 J 90= = 97,2739 mcg/cm²
3 , 14 cm ²

5.4.5. Perhitungan % difusi

terrtinggi /dosis
% Difusi = J ( t ) x 100%
luas lingkaran
 97,2739 mcg
x 100 %
= 2500 mcg
3 , 14 cm ²

97,2739 mg/cm ²
= x 100 %
796,1783 mgz/cm ²

¿ 12,2176 % = 12,22%

5.4.6. Kesimpulan

% Difusi gel piroksikan pirofel yang dihasilkan yaitu 12,22% dimana tidak
memenuhi syarat berdasarkan pustaka yaitu lebih dari 80% maka diperlukan
peningkat penetrasi

5.5. Grafik/Kurva Difusi Gel Piroksikam

Waktu (menit) Fluks/J(t) mg/cm2

5 11,9374 mcg/cm2
15 37,9835 mcg/cm2
30 39,9003 mcg/cm2
45 54,1029 mcg/cm2
60 70,6325 mcg/cm2
90 97,2739 mcg/cm2