DATA HASIL PRAKTIKUM

1. Tuliskan data pengukuran kadar BSA standar!

Kadar standar BSA (mg/ml) Absorbansi

0 0,057
1 0,172
2 0,268
3 0,359
4 0,437
6 0,615
8 0,782

2. Buatlah kurva standar BSA dan persamaan regresi liner standar BSA (perhitungan untuk
penentuan kadar protein sampel)!

Kurva Standar BSA

0.9
0.8
f(x) = 0.09 x + 0.08
0.7 R² = 1
0.6
Absorbansi

0.5
0.4
0.3
0.2
0.1
0
0 1 2 3 4 5 6 7 8 9
Kadar Standar BSA

Persmaan regresi linear: y = 0,0892x + 0,0785

3. Tuliskan data hasil pengukuran berat protein sampel (filtrat) hasil hidrolisis enzim!

Sampel Nilai Absorbansi Berat Kadar Protein (mg/ml)

Kedelai Mentah 0,821 1,002 0,083

Kedelai Rendam 0,735 1,0503 0,07

Kedelai Rebus 0,414 1,0092 0,037

Kedelai Sangrai 0,509 1.0203 0,047

Kecambah Kedelai 0,653 1,0321 0,062

Tempe Kedelai 0,576 1,0066 0,055
Susu Cair 0,733 1,02 0,072
Yogurt 0,697 1,00 0,069
Perhitungan :  Kedelai sangrai
Kadar protein filtrat = 0,509=0,0892x + 0,0785

Kadar Protein x FP x Volume Filtrat x=4,826

Berat sampel ( gr ) x 1000 4,826 x 1 x 10
=0,047
1,0203 x 1000
y = 0,0892x + 0,0785
 Kecambah kedelai
FP = 1 Volume Filtrat = 10 ml
0,653=0,0892x + 0,0785
 Kedelai mentah
x=6,441
0,821=0,0892x + 0,0785
6,441 x 1 x 10
x=8,324 =0,0624
1,0321 x 1000
8,324 x 1 x 10
=0,083  Tempe kedelai
1,002 x 1000
00,576=0,0892x + 0,0785
 Kedelai rendam
x=5,577
0,735=0,0892x + 0,0785
5,577 x 1 x 10
x=7,360 =0,0554
1,0066 x 1000
7,360 x 1 x 10
=0,070  Susu cair
1,0503 x 1000
0,733=0,0892x + 0,0785
 Kedelai rebus
x=7,337
0,414=0,0892x + 0,0785
7,337 x 1 x 10
x= 3,761 =0,0719
1,02 x 1000
3,761 x 1 x 10
=0,037  Yogurt
1,0092 x 1000
 0,697=0,0892x + 0,0785 6,934 x 1 x 10
=0,0693
1 x 1000
x=6,934

4. Hitung daya cerna protein sampel !

Jenis Sampel Kadar Protein hasil Kadar Protein Daya Cerna
hidrolisis enzimatis Sampel (kjiedahl) Protein (%)
(mg/gram) (mg/gram)
Kedelai Mentah 0,083 0,264 31,44
Kedelai Rendam 0,07 0,129 54,26
Kedelai Rebus 0,037 0,250 14,8
Kedelai Sangrai 0,047 0,298 15,77
Kecambah Kedelai 0,0624 0,108 57,7
Tempe Kedelai 0,0554 0,197 28,12
Susu Cair 0,0719 0,022 326,81
Yogurt 0,0693 0,013 533,08

 Kedelai mentah
% daya cerna =
 Kedelai sangrai
Kadar protein filtrat
× 100 %
Kadar proteinsampel % daya cerna =
= 0.083/0.264 x 100% Kadar protein filtrat
× 100 %
= 31.44% Kadar protein sampel
 Kedelai rendam = 0.047/0.298 x 100%
% daya cerna = = 15.77%
%Daya cerna =
Kadar protein filtrat
× 100 %
Kadar protein sampel
= 0.07/0.129 x 100% mg
kadar protein filtrat ( )
gram
= 54.26% x 100 %
mg
kadar protein sampel ( )
 Kedelai rebus gram
% daya cerna = 0,0624
= x 100 %
0,108
Kadar protein filtrat = 57,7%
× 100 %
Kadar protein sampel  Tempe kedelai
= 0.037/0.25 x 100%
%Daya cerna =
= 14.8%
 mg 0,0719
kadar protein filtrat ( ) = x 100 %
gram 0,022
x 100 %
mg = 326,81%
kadar protein sampel ( )
gram
0,0554  Yogurt
= x 100 %
0,197 %Daya cerna =
= 28,12%

mg
 Susu cair ( gram
kadar protein filtrat ) x 100 %
%Daya cerna = mg
kadar protein sampel (
gram )
0,0693
mg = x 100 %
kadar protein filtrat ( ) 0,013
gram
x 100 %
mg = 533,08%
kadar protein sampel ( )
gram

5. Tuliskan rumus daya cerna protein !

Penentuan kadar protein filtrat (metode biuret)
kadar protein x fp x volume filtrat
Kadar protein filtrat =
berat sampel ( gr ) x 1000

Penentuan daya cerna protein (Hasil biuret/metode Kjedahl)

mg
Kadar proteinfiltrat ( )
gram
% daya cerna = X 100 %
mg
Kadar proteinsampel ( )
gram