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Absorbansi

Sampel Konsentrasi
No. Tipe ( = 660
ID (mg/mL)
nm)
1 STD 1 standard 0.3 0.504
2 STD 2 standard 0.5 0.566
3 STD 3 standard 0.7 0.581
4 STD 4 standard 0.9 0.640

Kurva Standar Glukosa


0.700

0.600

0.500 y = 0.2115x + 0.4458


R = 0.9555
Absorbansi

0.400
Kurva Standar Glukosa
0.300
Linear (Kurva Standar
0.200 Glukosa)

0.100

0.000
0 0.2 0.4 0.6 0.8 1
Konsentrasi (mg/mL)

Persamaan y = ax + b
y = 0.2115x + 0.4458 0.4892
=x
0.2115
y = absorbansi sampel
2.3230 mg/mL = x
x = konsentrasi sampel
2.3230 mg
0.9350 = 0.2115x + 0.4458 1 =x
100
0.9350 - 0.4458 = 0.2115x 23.230 mg/100 mL
0.4892 = 0.2115x

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