Example standard curves Error types Author - PG Arthur

Standard curve - linear fit - triplicates Sheet locked - password: standard

x label y label 0.4

Concentration
Absorbance
(mg/ml)
0 0.049 f(x) = 0.0515765765765766 x + 0.0831981981981981
0 0.052
R² = 0.93022745933685

Absorbance
0 0.059
1 0.15
1 0.135 0.2
1 0.145
2 0.22
2 0.2
2 0.21
3 0.26
3 0.27
3 0.25
0
5 0.3 0 1 2 3 4 5 6
5 0.32
5 0.33 X label
y = ax + b

Insert numbers to match equation below a&b Average for

a b zero concentration % difference
0.0516 0.0832 0.053333 -56

Average Standard
Sample identity Measured absorbance Calculated concentration (mg/ml)
(mg/ml) deviation
A B C A B C
1 0.08 0.081 0.085 -0.06 -0.04 0.03 -0.023 0.051
2 0.085 0.09 0.095 0.03 0.13 0.23 0.132 0.097
3 0.226 0.225 0.224 2.77 2.75 2.73 2.748 0.019
4 0.25 0.24 0.245 3.23 3.04 3.14 3.136 0.097
Example standard curves Error types Author - PG Arthur

Standard curve - polynomial fit - triplicates

Solving a polynomial

x label y label 0.35 The solution for a polynomial of the fo

Concentration
(mg/ml) Absorbance
f(x) =0.3
− 0.00840697103583702 x² + 0.0942930780559647 x + 0.0547962690230731
0 0.049 R² = 0.991518862462941
0 0.052
0.25
0 0.059

Absorbance
1 0.15 0.2 For your data the formula is ax2 + bx +
1 0.135
1 0.145 0.15 Therefore the equation needs to be re
2 0.22 where y is absorbance and x is conce
2 0.2
0.1
2 0.21 The solution is
3 0.26
0.05
3 0.27
3 0.25
5 0.3
0
5 0.32 0 1 2 3 4 5 6
5 0.33 Concentration (mg/ml)

ax2 + bx + c = 0

Insert numbers to match equation below a&b Average for

a b c zero concentration % difference
-0.0084 0.0943 0.0548 0.0533333333 -2.75

Standard
Average deviation
Sample identity Measured Calculated concentration (mg/ml) (mg/ml)
A B C A B C
1 0.08 0.081 0.085 0.27 0.29 0.33 0.296 0.030
2 0.085 0.09 0.095 0.33 0.39 0.44 0.387 0.057
3 0.226 0.225 0.224 2.28 2.26 2.24 2.260 0.018
4 0.25 0.24 0.245 2.74 2.54 2.64 2.637 0.100
Example standard curves Error types Author - PG Arthur

Standard curve - linear fit - triplicates

f(x) = NaN x + NaN
x label y label R² = 0
Concentration 10
Absorbance
(units) 9
0 8
0
7

Y label
0
6
5
4
3
2
1
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
X label
y = ax + b

Insert numbers to match equation below a&b Average for

a b zero concentration % difference
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Average Standard
Sample identity Measured absorbance Calculated concentration (units)
(units) deviation
A B C A B C
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Example standard curves Error types Author - PG Arthur

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Example standard curves Error types Author - PG Arthur

Standard curve - polynomial fit - triplicates

Solving a polynomial

x label y label 1 The solution for a polynomia

Concentration (ug/ml) Absorbance
0.9
0 0.294
0 0.3 0.8 f(x) = − 9.75661375661376E-07 x² + 0.00146450793650794 x
0 0.3 0.7 + 0.321561904761905
R² = 0.940926876370711

Absorbance
150 0.534 For your data the formula is
0.6
150 0.556
150 0.618 0.5 Therefore the equation need
300 0.613 0.4 where y is absorbance and x
300 0.718
300 0.662 0.3 The solution is
450 0.791 0.2
450 0.672 0.1
450 0.771
600 0.839 0
600 0.89 0 100 200 300 400 500 600 700
600 0.88 Concentration (ug/ml)

ax2 + bx + c = 0

Insert numbers to match equation below a&b Average for

a b c zero concentration % difference
-1.00E-06 0.0015 0.3216 0.298 -7.91946309

Standard
Average C.V
deviation
Sample identity Measured Calculated concentration (units) (units)
A B C A B C
1 0.294 0.3 0.3 -18.18 -14.26 -14.26 -15.569 2.261 -14.5188607
2 0.534 0.556 0.618 158.31 177.20 234.15 189.886 39.481
3 0.613 0.718 0.662 229.33 342.45 278.73 283.500 56.710
4 0.791 0.672 0.771 444.88 289.46 413.70 382.677 82.222
5 0.893 0.89 0.88 Err:502 Err:502 685.97 Err:502 Err:502
6 1.186 1.292 1.276 Err:502 Err:502 Err:502 Err:502 Err:502
7 0.502 0.53 0.533 131.86 154.94 157.46 148.086 14.111
8 0.304 0.318 0.317 -11.64 -2.40 -3.06 -5.700 5.158
Example standard curves Error types Author - PG Arthur

9 0.574 0.628 0.658 193.13 243.94 274.50 237.190 41.101

10 0.556 0.607 0.588 177.20 223.60 205.85 202.215 23.411
-190.27 -190.27 -190.27 -190.266 0.000
-190.27 -190.27 -190.27 -190.266 0.000
-190.27 -190.27 -190.27 -190.266 0.000
-190.27 -190.27 -190.27 -190.266 0.000
-190.27 -190.27 -190.27 -190.266 0.000