Gluc

Volume Gluc conc when concentration

Volume of 0.1M gluc mass from
Test Tube of dH O mixed with when mixed Absorbance
2
glucose (mL) stock (mmol)
(mL) dH20 (M) with 9.0 ml
dH2O
1 3 0 0 0 0 0
2 2.5 0.5 0.05 0.016666666667 0.005 0.123
3 2 1 0.1 0.033333333333 0.01 0.269
4 1.5 1.5 0.15 0.05 0.015 0.37
5 1 2 0.2 0.066666666667 0.02 0.498
 Standard Curve
0.6
0.498
0.5
f(x) = 24.86 x + 0.0034
R² = 0.99761677234397 0.37
0.4
Absorbance

0.3 0.269

0.2
0.123
0.1
0
0
0 0.005 0.01 0.015 0.02 0.025
Concentration (M)