Calibration curves Solution A

Concentrations of
Absorbance at λmax Absorbance at λmax 260nm for PBS
fluconazole in PBS
260nm 0.05
Phase (µg/ml)
5 0.016 0.045
0.04
0.024
10 0.016
20 0.035
0.039

Absorbance
0.03
40 0.027
0.025 y = 0.0002x + 0.0191
60 0.026
0.02
80 0.038
0.015
100 0.044
0.01
0.005 m = 0.0002
0
0 20 40 60 80 100 120
Concentration
22

Funnel A (PBS)
A from question, Conc. From Graph
Absorbance 0.024
Concentration 22

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Calibration curves Solution B

Concentrations of
Absorbance at λmax 260nm for Octanol
fluconazole in Absorbance at λmax
Octanol Phase 260nm 0.2

(µg/ml) 0.18
5 0.016 0.16
10 0.015 0.14 0.068
20 0.035

Absorbance
0.12 y = 0.0019x - 0.0022
40 0.068 0.1
60 0.102 0.08
80 0.16 0.06
100 0.189
0.04
m = 0.002
0.02
0
0 20 40 60 80 100 120
Concentration
37

Funnel A (Octanol Phase)

A from question, Conc. From Graph
Absorbance 0.068
Concentration 37

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Question # 2

For Funnel A

Caq = 22
Corg = 37
P = ?

𝐶𝑜𝑟𝑔 37
𝑃= = = 1.682
𝐶𝑎𝑞 22

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