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  • Protein Estimation

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    This document describes a standard curve for total protein estimation. Various concentrations of protein standards from 0 to 2000 μg/mL were measured for absorbance at 562 nm. A linear relationship was observed between protein concentration and absorbance, with an equation of y = 0.00092598222786417x + 0.00174547762615 describing the trendline. This standard curve can be used to determine unknown protein concentrations based on their measured absorbance.

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    This document describes a standard curve for total protein estimation. Various concentrations of protein standards from 0 to 2000 μg/mL were measured for absorbance at 562 nm. A linear relationship was observed between protein concentration and absorbance, with an equation of y = 0.00092598222786417x + 0.00174547762615 describing the trendline. This standard curve can be used to determine unknown protein concentrations based on their measured absorbance.

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    6 views2 pages

    Protein Estimation

    Uploaded by

    tusherworkmail
    This document describes a standard curve for total protein estimation. Various concentrations of protein standards from 0 to 2000 μg/mL were measured for absorbance at 562 nm. A linear relationship was observed between protein concentration and absorbance, with an equation of y = 0.00092598222786417x + 0.00174547762615 describing the trendline. This standard curve can be used to determine unknown protein concentrations based on their measured absorbance.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    of 2

    Standard Curve for total protein Estimation

    Protein Standard (µg/mL) Average Absorbance (562 nm) Absorbance (562nm)


    2000 1.9515 1.857 Standard curve for total pro
    1500 1.501 1.4065 tion
    1000 0.9925 0.898
    2.5
    750 0.7905 0.696
    500 0.559 0.4645 2

    Absorbance (562nm)
    250 0.3195 0.225 f(x) = 0.00092598222786417 x + 0.001
    125 0.225 0.1305 1.5
    25 0.1275 0.033
    1
    0 0.0945 0
    Average Average 0.5
    0.729 0.6345
    0
    slope 0 500 1000 1500 2000 2500
    0.000925982227864 concentartion (µg/mL)
    Y intercept
    0.00174547762615
    tandard curve for total protein Estima-
    tion

    Average Absorbance
    (562 nm)
    f(x) = 0.00092598222786417 x + 0.0017454776261504
    Absorbance (562nm)
    Linear (Absorbance
    (562nm))
    Linear (Absorbance
    (562nm))

    0 500 1000 1500 2000 2500


    concentartion (µg/mL)

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