Adamson University

College of Engineering

Chemical Engineering Department

Laboratory Report

Spectrophotometry
Experiment 1

Experiment run on: February 13, 2021

Submitted by:

Andallo, Jan Arvee V. 201912478

Austria, Jeremy Kyle Edson A. 201914365

Castro, Sean Carlo S.A. 201914624

Chua, Ma. Angelica B. 201914283

Reyes, Joserie Joice P. 201914413

Group 7

Submitted to:

Pinky Joy Janaban

Physical Chemistry for Engineers 1 Laboratory

51010

February 17, 2021

II. VISIBLE SPECTRUM OF RED DYE (CONCENTRATION, 20ppm)

WAVELENGTH ABSORBANCE
(nm) (AU)
400 0
410 0.001
420 0.004
430 0.01
440 0.028
450 0.064
460 0.119
470 0.178
480 0.242
490 0.346
500 0.541 Mean 0.17692
520 0.996 Median 0.029
530 0.927 Mode 0.001

540 0.547
550 0.208
560 0.076
570 0.043
580 0.029
590 0.014
600 0.008
620 0.001
640 0.001
650 0.004
670 0.013
680 0.023
Table 1. Analysis of the Visible Spectrum of Red Dye
 ABSORBANCE OF RED DYE (20PPM)
1.2

0.8

0.6

0.4

0.2

0
400 450 500 550 600 650 700

Figure 1. Graphical Representation of the Absorbance of Red Dye

III. VISIBLE SPECTRUM OF BLUE DYE (CONCENTRATION, 20ppm)

WAVELENGTH ABSORBANCE
(nm) (AU)
375 Not within the range
400 0.126
410 0.112
420 0.077
430 0.044
440 0.019
450 0.007
460 0.002
470 0.007
480 0
490 0.001
500 0.002 Mean 0.18464
Median 0.065
520 0.014
Mode 0.007
530 0.025
540 0.039
550 0.065
560 0.12
570 0.211
580 0.311
590 0.397
600 0.504
620 0.886
640 0.858
650 0.6
670 0.141
680 0.048
Table 2. Analysis of the Visible Spectrum of Blue Dye
 ABSORBANCE OF BLUE DYE (20PPM)
1

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0
400 450 500 550 600 650 700

Figure 2. Graphical Representation of the Absorbance of Blue Dye

IV. DETERMINATION OF CONCENTRATION USING ABSORBANCE VALUES

WAVELENGTH, 520 nm
RED DYE
CONCENTRATION (PPM) ABSORBANCE
0 0
10 1.073
50 1.074
80 1.073
100 1.074

Mean 0.8588
Median 1.073
Mode 1.073
Confidence Interval ± 0.001

Table 3.1 Analysis of Absorbance using Concentration Values of Red Dye

 Beer's Law Plot: Concentration of Red Dye vs. Absorbance
1.600

1.400

1.200 1.073 1.074 1.073 1.074

1.000

Absorbance 0.800
Abs = 0.0136(Conc)
0.600 R² = -0.21

0.400

0.200
0
0.000
0 20 40 60 80 100 120
Concentration (ppm)

Figure 3. Beer’s Law Plot for the Concentration of Red Dye

Empirical equation: Abs = 0.0136(Conc)

Absorbance Absorbance Percentage Error
Concentration (Theoretical) (Experimental) (% error)
0 0 0 #DIV/0!
10 0.136 1.073 7.3
50 0.68 1.074 7.4
80 1.088 1.073 7.3
100 1.36 1.074 7.4

Table 3.2. Analysis of Theoretical Values for the Absorbance of Red Dye
WAVELENGTH, 620 nm
BLUE DYE
CONCENTRATION (PPM) ABSORBANCE
0 0
10 0.886
50 0.889
80 0.886
100 0.888

Mean 0.7098
Median 0.886
Mode 0.886
Confidence Interval ± 0.003

Table 4.1 Analysis of Absorbance using Concentration Values of Blue Dye

 Beer's Law Plot: Concentration of Blue Dye vs. Absorbance
1.200

1.000
0.886 0.889 0.886 0.888

0.800

Absorbance 0.600
Abs = 0.0112(Conc)
R² = -0.209
0.400

0.200

0
0.000
0 20 40 60 80 100 120
Concentration (ppm)

Figure 4. Beer’s Law Plot for the Concentration of Blue Dye

Empirical equation: Abs = 0.0112(Conc)

Absorbance Absorbance Percentage Error (%
Concentration (Theoretical) (Experimental) error)
0 0 0 #DIV/0!
10 0.112 0.886 11.4
50 0.56 0.889 11.1
80 0.896 0.886 11.4
100 1.12 0.888 11.2
Table 3.2. Analysis of Theoretical Values for the Absorbance of Blue Dye