DATA PENGAMATAN

1. Perhitungan Larutan Stok Baku Pembanding Paracetamol

Penimbangan = 15 mg dalam 50 mL
15 mg
Konsentrasi =
50 mL
= 0,3 mg/mL
= 300 ppm

2. Pembuatan kurva

Volume larutam stok Konsentrasi

Absorbansi
yang dipipet (mL) (mg/100mL)

1 3 0,144
1,5 4,5 0,248
2 6 0,308
2,5 7,5 0,446
3 9 0,519
3,5 10,5 0,636
4 12 0,683

a = -0,0387

b = 0,062

r = 0,9956

Persamaan regresi linear:

y = a ± bx
y = -0,0387 ± 0,062 x
1. (1 ml) 2. (1,5 ml)
V1.N1 = V2.N2 V1.N1 = V2.N2
 1 . 300 = 100 . N2 1,5 . 300 = 100 . N2
N2 = 3 ppm N2 = 4,5 ppm

3. (2 ml) 4. (2,5 ml)

V1.N1 = V2.N2 V1.N1=V2.N2
2 . 300 = 100 . N2 2,5 . 300 = 100 . N2
N2 = 6 ppm N2 = 7,5 ppm

5. (3 ml) 6. (3,5 ml)

V1.N1=V2.N2 V1.N1=V2.N2
2,5.2000 = 100. N2 3,5 . 300 = 100 . N2
N2 = 9 ppm N2 = 10,5 ppm

7. (4 ml)

V1.N1=V2.N2

4 . 300 = 100 . N2

N2 = 12 ppm

Kurva Kalibrasi
0.8
0.7
0.6
0.5 Series 1
0.4
0.3
0.2
0.1
0
3 4.5 6 7.5 9 10.5 12
3. Pembuatan larutan uji paracetamol

Kekuatan sediaan = 120 mg/5 ml

120 mg x
=
5 ml 2 ml

120 mg.2 ml
x=
50 ml

¿ 48 mg

4. Uji stabilitas dipercepat

 Suhu 60˚C

Persamaan regresi linear

y+ a
y= a ± bx  x =
b
y= -0,0387 +0,062 x
r= 0,9956
−0,3860+0,062
1. t10 → x= = 4,9468
0,268
−0,0387=0,062
2. t30 → x= = 5,1806
0,2825
 −0,0387−0,062
3. t60 → x= =4,8339
0,261
−0,0387−0,062
4. t90 → x= =5,1242
0,279
−0,0387−0,062
5. t120 → x= = 5,2855
0,2855
−0,0387−0,062
6. t150 → x= = 5,7049
0,315

 Suhu 70˚C

Persamaan regresi linear

y= a ± bx
y= -0,0387 +0,062 x 2
r = 0,9956
0,062−0,0837
1. t10 → x= = 4,7613
0,2565
 0,062−0,038
2. t30 → x= = 4,9387
0,2675
0,062−0,0387
3. t60 → x= =5,2290
0,2855
0,062−0,0387
4. t90 → x= =5,0839
0,2765
0,062−0,0387
5. t120 → x= = 4,9468
0,268
0,3062−0,0387
6. t120 → x= = 5,6
0,3085

 Suhu 80˚C

Persamaan regresi linear

y= a ± bx
y= -0,387 +0,062 x
r = 0,9956
0,062−0,0387
1. t10 → x= = 4,7774
0,2575
 0,062−0,0387
2. t30 → x= = 4,3661
0,232
0,062−0,0387
3. t60→ x= =5,0758
0,276
0,062−0,0387
4. t90 → x= =5,3097
0,2905
0,862−0,0387
5. t120 → x= = 5,1081
0,278
0,062−0,0387
6. t120 → x= = 5,3258
0,2915

5. Tabel orde reaksi

 Suhu 60˚C
 Suhu 60° Orde 0
8.0000

7.0000

6.0000

5.0000

4.0000

3.0000

2.0000

1.0000
t10 t30 t60 t90 t120 t150

Suhu 60° Orde 1

2.5

1.5

0.5

0
t10 t30 t60 t90 t120 t150
 Suhu 60° Orde 1
1
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
t10 t30 t60 t90 t120 t150

 Suhu 70˚C
 Suhu 70° Orde 0
8

1
t10 t30 t60 t90 t120 t150

Suhu 70° Orde 1

2.5

1.5

0.5

0
t10 t30 t60 t90 t120 t150
 Suhu 70° Orde 1
1
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
t10 t30 t60 t90 t120 t150

 Suhu 80˚C
 Suhu 80° Orde 0
8

1
t10 t30 t60 t90 t120 t150

Suhu 80° Orde 1

2.5

1.5

0.5

0
t10 t30 t60 t90 t120 t150
 Suhu 80° Orde 1
1
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
t10 t30 t60 t90 t120 t150

6. Perhitungan orde reaksi

 Metode grafik dengan melihat nilai R

Orde reaksi ditemukan berdasarkan nilai R pada setiap suhu masing-

masing orde yang mendekati 1 atau -1. Maka, berdasarkan data diatas,
indometasin memiliki orde 0 karena pada suhu 60˚C mendekati 1 atau -1.
7. Menghitung energi aktivasi menggunakan rumus arrhenius

I
Persamaan regresi dan ln K
T
y= a ± bx
a= -2,6975
b= -930,5
r= -0,6502
y = -2,7044 - 913 x

1. Suhu 60˚C
1
=0,0030
60 °+273

2. Suhu 70˚C
1
=0,0029
70°+273

3. Suhu 80˚C
1
=0,0028
80 °+ 273
 Persamaan arrhenius
Ea I
ln k = ln A – [ × ]
R T
y= a ± bx
Maka Ea = b.R
Ea = -930,5 x 1,9872
= -1849,0896 kal/mol

8. Menghitung nilai k pada suhu kamar

Ea I
ln k25 = ln A - [ × ]
R T
I
ln k25 = -2,6975 - [930 , 5 × ]
25=273
ln k25 = -5,82
k25 = 2,9676 x 10−3

9. Menentukan kadaluarsa pada suhu kamar apabila kadaluarsa = terurai

10%
Diket: C0 = 4,9468 mg
90
90% = × C0
100
90
= × 4,9468
100
= 4,45212 mg → nilai Ct

Waktu kadaluarsa
Persamaan orde .....
Maka, Ct = C0- Kt →
-kt = Ct-C0
 CO−CT
t=
K
4,45212 = 4,9468-= 2,9676 x 10−3 .t

4,9468−4,45212
t: −3
=1666939 jam /¿ (6,9456 hari )
2,9676 x 10

Perhitungan orde reaksi

 Suhu 60˚C
Orde 0: Orde 1: Orde 2:
a = 4,8392 a = 1,5789 a = 0,2058
b = 0,0044 b = 0,0008 b = 0,0002
r = 0,7800 r = 0,7769 r = -0,7718

 Suhu 70˚C
Orde 0: Orde 1: Orde 2:
a = 4,7843 a = 1,5669 a = 0,2084
b = 0,0040 b = 0,0008 b = -0,0002
r = 0,7352 r = 0,7363 r = -0,7357

 Suhu 80˚C
Orde 0: Orde 1: Orde 2:
a = 4,5880 a = 1,5231 a = 0,2181
b = 0,0053 b = 0,0011 b = -0,0002
r = 0,7738 r = 0,7634 r = -0,7513