Faculty of Dentistry

Oral biology department

HPT231
Oral Biology 1
Lab manual
 Faculty of Dentistry
Oral biology department

HPT231
Oral Biology 1
Lab manual
Pr. Sahar Shawkat
Head of oral biology department

Mohamed Shamel
Assistant Lecturer
 Evaluation sheet
Name:

ID: Group:

Drawing Date Signature

Bud stage
Cap stage
Early bell stage

Late bell stage

Root formation
LS Ground
LS Decalcified
TS Ground crown
TS Ground root
Pulp cells
Periodontal
ligament
Bone
Evaluation 1
Evaluation 2
FINAL EVALUATION
 Index
Tooth development
Bud stage…………………………………….. 1
Cap stage …………………………………….. 3
Early bell stage…………………………….. 5
Late bell stage……………………………… 7
Root formation……………………………. 9
Enamel........................................................... 13
Dentin………………………………………………………. 14
Longitudinal ground section in tooth…………. 15

Transverse section in tooth………………………… 17

Longitudinal decalcified section in tooth …… 21

Pulp cells…………………………………………………….. 23

Periodontal ligament………………………………….. 27

Alveolar bone
Macroanatomy……………………………. 31
Microanatomy…………………………….. 33

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 Oral Biology I Tooth development

Bud stage

Oral Epithelium

Dental Sac
Dental lamina
Dental Papilla

Enamel organ

1
Oral Biology I Tooth development

Bud stage

2
 Oral Biology I Tooth development

Cap stage

Oral Epithelium

O.E.E.
Enamel cord

Enamel Knot

Stellate Reticulum

Cell free zone I.E.E.

Dental Papilla

Dental Sac

3
Oral Biology I Tooth development

4
 Oral Biology I Tooth development

Early bell stage in a deciduous tooth

Oral Epithelium

Lateral dental
lamina
O.E.E.
Main dental
lamina Stellate Reticulum

Stratum
intermedium

odontoblasts
I.E.E.

Cervical loop
Dental Papilla

Dental Sac

5
 Oral Biology I Tooth development

Early bell stage in a deciduous tooth

6
 Oral Biology I Tooth development

Late bell stage in a deciduous tooth

1- oral epithelium 8- ameloblast 15- dental papilla

2- main dental lamina 9- inner enamel epithelium 16- cervical loop

3- epithelial rests of Serres' 10- enamel matrix 17- dental sac

4- successional dental lamina 11- enamel space

12- dentin
5- outer enamel epithelium
13- pre-dentin
6- stellate reticulum
14- odontoblast
7- stratum intermedium
7
 Oral Biology I Tooth development

Late bell stage in a deciduous tooth

6
8
10

12

15
8
Oral Biology I Tooth development

Root formation

9
Oral Biology I Tooth development

Root formation

10
Oral Biology I Tooth development

Root formation

11
Oral Biology I Tooth development

Root formation

12
Oral Biology I LS tooth

Enamel

13
 Oral Biology I LS tooth

Dentin Dentin
Ground section Decalcified section

14
 Oral Biology I LS tooth

Longitudinal Ground section in a deciduous tooth

Inc .lines of Retzius

Enamel Lamella
Enamel Spindle

Neonatal line

Interglobular dentin
space
Neonatal line
DEJ
Contour line of
Owen
CEJ

Incremental lines of
von Ebner
Tome`s
Pulp Space Granular layer

Incremental lines of Acellular

Salter cementum
Dentinal Tubules

Cellular cementum
Lacuna of
15
cementocytes
 Oral Biology I LS tooth

Longitudinal Ground section in a deciduous tooth

16
 Oral Biology I TS in tooth

Transverse Ground section in crown of a

deciduous tooth

A- Incremental lines of
Retzius

B- Enamel tuft

C- Enamel lamella

D- DEJ

17
 Oral Biology I TS in tooth

Transverse Ground section in root

Apical third Cervical third

Lacuna of cementocytes
Incremental lines of
Salter

Tome`s Granular layer

Dentinal Tubules

Lacuna of cementocyte A-Dentinal tubules

B-Acellular cementum

18
 Oral Biology I TS in tooth

Transverse Ground section in crown

Transverse Ground section in root

Apical third Cervical third

19
 Oral Biology I TS in tooth

Hunter-Schreger bands

D E

20
 Oral Biology I LS tooth

Longitudinal decalcified section in a deciduous tooth

Enamel space

Interglobular dentin

Neonatal line Pulp core

Contour line of Owen CEJ

Incremental lines of von

Ebner Dentinal Tubules

Acellular
cementum
Incremental lines of
Salter

Cellular
cementocytes cementum
21
 Oral Biology I LS tooth

Longitudinal decalcified section in a deciduous tooth

22
 Oral Biology I Pulp cells

Odontoblast
Intertubular Periodontoblastic Peritubular
dentin space DEJ
predentin dentin

Odontoblastic Lateral branches Terminal branches

process

23
 Oral Biology I Pulp cells

Odontoblast

Dentinal
Odontoblastic process Peritubular
tubules lumen
dentin

Periodontoblastic
space

Intertubular dentin

T.S. in dentinal tubules

24
 Oral Biology I Pulp cells

Pulp cells
1- Formative:

a) Odontoblast

b) Fibroblast

2- Defensive: 3- Rouget (Pericyte) cell

a) Mast cell

b) Histiocyte

4- U.M.C.
c) Eosinophil

d) Lymphocyte

e) Plasma cell

25
Oral Biology I Pulp cells

Pulp cells

26
 Oral Biology I Periodontal ligament

Periodontal ligament

2
3
4
7
5

1-Gingival fibers
2-Transeptal (interdental) fibers
3-Alveolar crest group
4-Horizontal group
5-oblique group
6-apical group
7-interradicular group
27
Oral Biology I Periodontal ligament

Periodontal ligament

1
2
3
4
7
5

6
28
 Oral Biology I Periodontal ligament

Periodontal ligament cells

U.M.C.
Fibroblast

Cementoblast Osteooblast

Lymphocytes Mast cell Histiocyte

Epithelial rests of Malassez

Osteoclast

29
Oral Biology I Periodontal ligament

Periodontal ligament cells

30
 Oral Biology I Alveolar Bone

Macroanatomy of alveolar bone

Outer Alveolar bone Bundle bone

cortical plate proper
Supporting Lamellar bone
alveolar bone
Inner cortical
Spongiosa plate

Basal bone

Mandibular canal

31
 Oral Biology I Alveolar Bone

Macroanatomy of alveolar bone

32
 Oral Biology I Alveolar Bone

Microanatomy of alveolar bone

Cementum
Zuckerkandl &
Hirschfeld
canals
Sharpey`s
fibers

Periodontal
ligament

Bundle Spongiosa Cortical plate

bone
Alveolar bone
Supporting alveolar bone
proper
Lamellar
bone

33
 Oral Biology I Alveolar Bone

Microanatomy of alveolar bone

34
Tooth development

35