JOURNAL OF EXPERIMENT

Title of Experiment : Bile

Day/Date of Experiment : Tuesday, December 1st 2020

Name/ID : Andi Evi Febrianti/1813440002

Class/Group : ICP of Chemistry Education/ICP 1.1

Members : 1. Adi Surya Abdi

2. Nurazizah Irwansyah

3. Chalifatul Ashila

4. Karina Silele Parampo

Assistant : Aan Eko Putra

A. OBJECTIVE OF EXPERIMENT
To know the physic condition of bile, musin test and anorganic compound
in bile, relationship between color substance in body, Van Den Berg test and bile
acid.

B. APPARATUS AND CHEMICALS

1. Apparatus
a. Graduated cylinder 10 mL 1 piece
b. Graduated cylinder 25 mL 1 piece
c. Test tube 6 pieces
d. Test tube rack 1 piece
e. Beaker glass 100 mL 3 pieces
f. Spray bottle 1 piece
g. Tweezers 1 piece
 h. Test tube clamp 1 piece
i. Drop pipette 1 piece
j. Scissor 1 piece
k. Analytical balance 1 piece
l. Spatula 1 piece
2. Chemicals
a. Indicator universal
b. 10% Acetic acid CH3COOH
c. Barium chloride BaCl2
d. Silver nitric AgNO3
e. Ammonium molibdat
f. Aquadest H2O
g. Nitric acid HNO3
h. Ebrlichdiazo
i. Blanco
j. Sucrose C12H22O12
k. Sulfuric acid H2SO4
l. Tissue

C. WORK PROCEDURES
1. Bile physical test

Universal
indicator

Observation of Measured pH Weight bile

bile: color, bile
smell and shape
2. Tests of musin and inorganic compounds in bile

25 mL musin filtrate
1 mLCH3COOH Filtrate musin
bile a

Investigation:
a. Chloride with AgNO3 solution

filtrat AgNO
b. Sulfate with
3 BaCl2shake
Solution

filtrat BaCl2 shake

b. Phospate with ammonium molibdate

filtrat (NH4)3Mo7O24 shake

1. Bile dye test
a. Gmelin

Write the result

3 mL HNO3
constrated bile

b. Smith test

Observation ring blue or

green

bile I2 in alkohol

2. Van Den Berg test

Observation and write the

result

a.
2 mL 5 mL bile
ebrlichdiazo

b. Blank

Observation and write the

result

2 mL 5 mL bile
ebrlichdiazo
3. Acid bile test
a. H2SO4 concentrated

Observation
and write the
result
3 ml bile Kristal 3 ml H2SO4
solution (1:3) sukrosa

b. Molish reactor

Observation
and write
the result
3 ml bile (1:3) Crystal of 3 ml Molisch
sucrose solution

Makassar, 2020
Assistant Apprentice,

Aan Eko Putra Andi Evi Febrianti

ID. 1613041006 ID. 1813441003