Timog, Keesha Mae U.

BSN1-A

Questions:
What are the differences between the three levels of disinfectant effectiveness?
The differences between the three levels are the High-level germicides have the ability to kill
vegetative cells, fungi, viruses, and endospores, leading to sterilization, with extended use.
Intermediate-level germicides, as their name suggests, are less effective against endospores and
certain viruses, and low-level germicides kill only vegetative cells and certain enveloped viruses,
and are ineffective against endospores.

When comparing the activities of two disinfectants against the same microbe, using the disk-
diffusion assay, and assuming both are water soluble and can easily diffuse in the agar, would a
more effective disinfectant have a larger zone of inhibition or a smaller one?
The more effective disinfectant is the larger zone of inhibition

Is the use-dilution test performed in a clinical setting? Why?

No, because it is a manufacturers test

What does a positive in-use test indicate?

Growth of 5 or more colonies on either plate suggest that viable microbial cells existed in the
disinfectant solution and that it is in fact contaminated.