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KATRAL BIO ENZYME


TELEGRAM GROUP
BIO ENZYME
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Who invented the Bio Enzyme?


Bio Enzyme is developed by
Dr . Rosukun Poompanvong from
Thailand. However, although
called an Enzyme, it is not an
enzyme in technical sense. Also,
the term Garbage Enzyme (another
name by which it is called) can
also be misleading! Some call it as
Fruit enzyme... but Fruit Enzyme
and Garbage Enzyme are different;
Fruit Enzymes are editable while
Bio enzymes are not.
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How to make Bio Enzyme or


Garbage Enzyme for household
cleaning purposes
The questions are divided into three
parts so that it’s easier for you to
assimilate the information as and
when you need it:
1. before, when you need know
what are Bio Enzymes and how
exactly to make them
2. during, when you might come
across issues or doubts about
whether it is progressing well for
you and
3. after, when you might want to
explore the many different uses of
the Bio Enzyme
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Do go through this list to get a good


understanding about Bio Enzymes
and get armed with the necessary
information to make it really work for
you.

Part 1:

Here are the Q & As you will need to


go through BEFORE you make the
Bio Enzyme

1. What are Bio Enzymes?


Bio Enzyme (also referred to as
Garbage Enzyme or Fruit Enzyme) is
a multi-purpose, natural cleaner
produced from vegetable/fruit peels
(usually citrus) or waste. It is an
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effective alternative to harsh


chemicals such as bleach, phenyl,
and other chemical solutions we
typically use in households to wash
our bathrooms, clean toilets, wipe our
floors, tiles and other surfaces.
Chemically, the Bio Enzymes are a
mixture of complex organic
substances such as proteins, salts
and other materials that are by-
products of the bacteria/yeast
(naturally occurring) that we will use
to make the Bio Enzyme. These
organic substances which are in the
Bio Enzyme are capable of breaking
down chemical and other organic
waste thus helping us in removing
stains, odor, getting rid of other
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harmful microbes, etc. They also


greatly neutralize toxins and
pollutants. In short, they are the next
best thing to be discovered by man
after a ripe and sweet Mango!

2. Why do we need to use Bio


Enzyme instead of chemicals and
solutions like Harpic, Mr. Muscle,
Phenyl, etc.?
You might have read all the recent
news about lakes frothing, lakes
catching fire, sewage contaminating
water bodies and so on and so forth.
As human population grows and our
lifestyles becomes increasingly
convenience driven, we are polluting
our living environment more and
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more. A polluted environment is in no


way conducive to a healthy living –
causing us disease, disturbance and
distress. As people who are
conscious of our footprint on the
environment, we must do all that we
can to not just stop adding to the
pollution but also to prevent it
actively. One of the most important
and easy way to do that is to
completely eliminate all chemicals
from your household and replace
them with Bio Enzyme and its
variations.

Moreover, it is in our personal best


interest as well to switch to the Bio
Enzymes! Chemical cleaners, in
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addition to polluting the environment,


also leave chemical residues in your
household – potential and slowly
accumulating toxins that can cause
great harm to children, pets and
adults as well. A recent news article I
read cited the residue of dishwash
bars on vessels as one of the major
causes of cancer (as they can turn
carcinogenic)!! While we do not know
the scientific validity of such claims, it
is always easier to be safer than
sorry. It’s even more safer to go
green completely!

3. What are the uses of Bio


Enzyme?
Bio Enzymes has a veritable list of
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uses – I keep discovering more and


more uses to it!

Let me try to bracket them for you:


1. As a surface cleaner: Floor
Cleaner, Tile Cleaner, Toilet Cleaner,
Stove/Chimney Cleaner
(concentrated version or the pulpy
residue), Window Cleaner, Car wash,
etc.

2. As a dirt remover: Laundry liquid


(with or without other additions such
as soapnut), Vessel Cleaner (with or
without other additions such as
soapnut), stain remover, etc.

3. As a personal care product (Watch


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Katral Bio Enzyme Beautycare


Videos):
Body wash, Shampoo, Hair
conditioner, Shoe odor remover,
Sanitizer, Face Cream, Lotion…

4. As an anti-bacterial and anti-viral:


Refrigerator cleaner, Cabinet cleaner,
Unclogging drains

5. Washing Vegetables and Fruits to


neutralize harmful fertilizers/pesticide
residues on them
6. Pest Control: Continued use of Bio
Enzyme have been known to repel
small insects, ants, cockroaches at
home (though it doesn't kill them).
Also, Bio Enzyme is being used
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extremely effectively in fumigation


along with Soapnuts and Neem Oil.

7. Plant Care: It is a wonderful bloom


nutrient. Also helps in managing plant
pests like white flies, mealy bugs and
spider mites.There may be many
more uses that may just need to be
discovered!

5. How do we exactly make Bio


Enzymes?
Very simple. Remember the ratio
1:3:10 – Jaggery to Citrus Peels to
Water. That is take one part of
Jaggery, 3 parts of Citrus peels and
10 parts of Water. If you use one cup
of Jaggery, use the same cup to
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measure the other two ingredients as


well. But you need not be 100%
accurate to the last dot – the Bio
enzyme is forgiving if you add a bit
more of this or that (not too much or
too less though!). Take a big enough
and air tight container with a lid which
can accommodate this solution + still
have a 10 – 15 % space left empty.
Mix these three ingredients in that
container, put the lid on, label it with
the date of creation and leave it in a
dark place (such as inside your
kitchen cupboard or under the sink).
Once the solution starts fermenting
(will be explained in a bit), it will start
releasing some gases that will get
built up in the empty space in the
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container. Before this gas becomes


too much and “pops” outside, you will
need to release them. Hence, you will
need to open the lid once in a day
(for first one week at least), air it out
for a minute or so (no need to stir or
do anything else!), put the lid back
on, keep it back in the dark place and
again forget it for another day. From
second week onwards, the gas
activity will reduce a bit and you need
not open every day - you can open
every other day.

6. Can I use a metal or glass


container instead of plastic?
No, not advisable as metal and glass
will not be able to expand. There are
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gases that will get released during


the process of production – so plastic
is a better option. You can use any
old plastic container lying around.

7. Why is an airtight container


required?
The airtight container is necessary to
promote fermentation (minimize
oxygen) and to also avoid flies from
getting in.

8. How long before my Bio Enzyme


will be ready?
Usually, it will take three months for
the Bio Enzyme to be produced if you
do not use any other additional
ingredient other than that which are
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mentioned above (Jaggery, Citrus


Peels and Water). That is why it is
important that you label the container
with the date of creation. This way,
you can start multiple batches and
stagger them so that you can get a
continuous supply.

9. What is the rationale for the


stipulated 3 months’ period?
During the first month of the
fermentation, alcohol will be
released, so you will smell alcohol
when you open the container. In the
second month, you will smell an
acidic smell, which is the smell of
acetic acid. With many compounds
such as minerals and vitamins, it will
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continue breaking down and naturally


form the enzyme. Hence, the
minimum duration suggested is 3
months.

10. Does the Bio Enzyme have an


expiry date?
No, you can use the Bio Enzyme
lifelong! They do not get spoilt, go
bad or anything like that. Of course,
you need to ensure you keep them
safely closed away from pests and
flies.

11. What are the fruit peels I can


use to make Bio Enzyme? Can I
use Banana and Mango?
While the Bio Enzyme can be made
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with all the Fruits peels, for greater


household cleaning abilities, it is
usually recommended only to use
Citrus Fruit peels – i.e. Lemon,
Orange, Lime and Pineapple. You
can either use all four of them
separately in different batches to test
which is more effective or combine
them into one. As mentioned, you
can use Banana and Mango – but
they won’t be as effective as
Citrus. Plus, they may not smell as
great as citrus!

12. Can I use the entire fruit


instead of just the peels?
Yes, you can but why would you want
to “waste” the whole fruit instead of
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consuming it? But if the fruit is rotten


or spoilt, you can put it in whole or
cut up, not a problem.

13. Should I wash the peels before


I use them to make Bio Enzymes?
No, not required. During the process
of creating the Bio Enzyme, all
chemical residues, if any, will get
neutralized completely.

14. Does the fruits/peels I must


use to make the Bio Enzymes be
organic?
Again, same answer as above. All
chemical residues will get neutralized
during the process – so it doesn’t
matter if it is organic or not.
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15. Why is regular opening and


closing of the container needed?
Biogases will be produced as by-
products of the anaerobic breakdown
of the sugars (usually hydrogen,
CO2, and less likely methane). Thus,
releasing the gases produced,
especially during the initial stages, is
essential.

16. Do I really need to open the Bio


Enzyme every day? What will
happen if I forget to stir for a few
days?
No, not every day but every other day
definitely – at least for the first 3 – 4
weeks when the bacterial activity will
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be at its peak. Slowly, the speed of


the process will decrease and the
amount of gases that will get
produced will also decrease – so you
can increase the gap between the
time you go and check out your
Enzyme from 2 days (for fist 3-4
weeks) to 4 days (for next 3-4 days)
and then to once a week.

17. What is the purpose of brown


sugar or Jaggery in the process?
The purpose of Jaggery or molasses
or brown sugar is to provide “simple”
carbon source or energy for the
microorganisms to first feed and
grow. When growth is sizeable, the
microbes may then turn their
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attention to the additional food source


(the waste materials or peels) that is
harder to digest due to the complexity
in their molecular structures. This
process is usually energy consuming
for the microorganisms, and the
added sugar may provide them the
extra energy needed for this.

18. Can I use White Sugar instead


of Jaggery or Brown Sugar?
No, you can’t.

19. Can I use dried peels instead of


fresh ones?
No, it’s preferable that you use fresh
peels as much as possible
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20. Can I use Frozen and Thawed


peels?
Well, if you can’t get access to fresh
peels, you may use frozen and
thawed ones.

21. What do I do after three


months?
Take another container (such a big
enough vessel or bucket), filter out
the liquid from the Bio Enzyme
container using a fine mesh cloth or
filter, squeeze the pulp to take out
remaining liquid and voila – the Bio
Enzyme is ready! The liquid (which
should be clear and orange to
brownish in color) you have collected
in the other container is the Bio
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Enzyme – it might still have some


very fine residue which will settle
down once you keep it undisturbed
for some time – you can use along
with the residue, not a problem. You
can retain the pulp to start another
batch (along with fresh peels of
course) or to use as a concentrated
cleaner. Store both the liquid and the
pulp safely in closed containers for
everyday use.

22. I use an herbal floor cleaner


and find it extremely effective.
Why should I shift to Bio Enzyme?
Can you be 100% certain that the
“herbal” cleaner you use does not
have any chemical whatsoever? And
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that it contains only totally natural


substances? Please verify that.
Clever marketing and use of words
such a “herbal”, “organic”, “all
natural” are becoming more and
more commonplace by companies
that are out to fool customers. Please
do not fall pray to such tactics. Once
you have verified the authenticity of
your herbal cleaner, check whether
they are safe to use and the residues
they leave are safe to use. If you are
fully satisfied, you may continue to
use your herbal cleaner – but at a
cost! But I wonder why you would do
that when you can make your own
cleaner with the least and minimum
cost which will be equally effective if
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not more? What’s more, you can be


super proud as well.

Part 2: During the process of making


the Bio Enzyme

23. I’m curious to know the


scientific principle behind Bio
Enzyme. Can you explain?
Chemically, Bio Enzymes are a
mixture of concentrated Vinegar or
Alcohol (or a mixture/blend of both,
along with other organic compounds)
produced by microorganisms which
are naturally occurring in the
environment that were a part of the
solution you prepared. It is a simple
fermentation process where the food
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waste is broken down into smaller


compounds along with the release of
gases. Once all the food (Jaggery
and what is in Citrus that you added)
is digested by the microbes, they will
have nothing to eat and will die
naturally. But what they leave behind
(as their waste product so to speak)
is precious - the Bio Enzyme. As they
say, one man's waste is another
man's gold. In this case, the microbes
do all the hard work and we get to
enjoy their labour!

24. I see a white layer, like Fungus,


forming on top of the peels! What
do I do?
This is a good indication. Simply
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ignore the layer and keep airing out


the Enzyme-in-process. The white
layer is nothing but naturally
occurring yeast that has come up for
air. Do open it more frequently and
stir in the white mixture every once in
a while.

25. I have got worms in my Bio-


Enzyme! Help me!
Uh-uh, someone has not been
careful in keeping the lid tightly
closed! So the flies have come in and
laid their eggs! Well, not to worry,
add some more Jaggery, put the lid
back on and keep it very tight. Open
and close once in 2-3 days (beware
of the wriggly worms though
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harmless they are!). The worms will


go away by themselves. Don’t ask
where!

26. Is my Bio Enzyme supposed to


smell a certain way? How do we
get to know if the process is
completed?
Not really, since we will be using only
citrus, they will only smell nicely
citrusy.

As for how to know when it is ready:


Three factors.
1. Time - As mentioned, it takes 3
months to make the Bio Enzyme
without any yeast. If one adds yeast,
it can get done in 20-25 days
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(provided right environment is


maintained for the microbes to
flourish). This has been calculated
based on lot of people's experience
and research.
2. Stopping of Gas production - As
the fermentation slows down, so will
the production of gases from the
solution. At the end of the time
period, most of the food materials, if
not all, would have been converted
by the microbes into Vinegar or
Alchohol and there wont be anything
left for them to munch. So they will
start to de-activate or die. This
signals that solution is ready.
However, one needs to use this
indicator along with the time as
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sometimes fermentation might slow


down in between also due to
unfavorable conditions (we will see
more details of this below).
3. Peels settling down - the Peels we
used to make the Enzyme - as they
get processed by the micorbes (in
essence, eaten up) - will start to
settle down to the bottom of the
container. This is a good indicator -
sometimes, some peels will continue
to float while most settle down. That
is okay.

27. What If your enzyme becomes


very smelly like the smell of rotten
eggs or feces?
Just add one portion of brown sugar.
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For instance, this bottle contains one


liter of water. Then you need to
quickly add 100 grams of brown
sugar to it. Stir it thoroughly and then
cover the cap tightly. After you seal it,
do not open it again. One month
later, you will find that it’s good again
when you open it.

28. What are the Gases that will


get released when we make the
Bio Enzymes?
Some of the gases that will be
produced by Bio Enzymes are CO2,
O3, NO3, CO3, etc. None of these
gases are toxic or unsafe. Moreover,
only negligible quantities of these
gases will be produced during the
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process.

29. So what are some of the


"unfavorable" things that can
happen that can slow down or
affect the Bio Enzyme production
process?
1. Leaving your container open and
letting in too much oxygen - As
mentioned, fermentation releases
carbon di oxide along with minor
quantities of other gases. This CO2
needs to be let out while letting in bit
of Oxygen. However, if we
completely leave the container open,
too much aerobic condition will affect
the final quality of the Enzyme
produced.
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2. Worms and Flies in your Bio


Enzyme container - this will also
occur only if you had NOT been
careful about your container and left
it open at some point inviting flies to
come in and breed. This can slow
down fermentation to a great extent
and take away the food meant for
microbes. We can try to correct when
worms occur (we need to add extra
jaggery and by closing container in
air tight manner for 1 week -
hopefully, the worms will die.
Otherwise, one just needs to
abandon and start again.

3. Temperature - Microbes like a


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temperature that is close to our body


temperature. So if we are able to
maintain that in the solution, then the
fermentation will happen pretty fast.
But if we create extreme temperature
fluctuations or worse make it too hot
or too cold, fermentation will get
affected. For example, keeping the
Enzyme inside your fridge - thats a
big NO. Keeping your Enzyme in
direct sunlight - that's a big NO.
Shifting your container continuously
from place to place causing lot of
temperature fluctuations - that's a
BIG No. Tip: Try to keep the Enzyme
under the sink in your kitchen
(relatively warm place in your house)
or even on top of your fridge (where
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heat is generated) protected by a


cloth underneath.

4. Forgetting to open for months and


months - Now, just the way leaving
your container totally open is also a
problem, not opening at all can also
be a disaster. The built up gas, for
one, can cause an explosion! Two -
the accumulated CO2 will end up
killing the microbes as well. Hence,
the suggestion to open it once a
week initially (when fermentation rate
if at its highest). After that, when
fermentation slows down and gas
production slows down, you can open
it once in two or three days.
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Part 3: AFTER your Bio Enzyme is


ready

30. My Bio Enzyme is ready. Do I


need to use it right away?
When you are done making the
enzyme, you don’t need to use it right
away. You can keep it for years - one
year, two years, three years, even 10
or 20 years. The longer the period is,
the smaller the molecules become,
as the mixture continues to ferment
and decompose. When the
molecules get smaller, it will have a
better penetration. Definitely, longer
durations would be more ideal to
ensure a more complete fermentation
process where there will be no (or
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little) sugars left, and higher


concentrations of products could be
achieved. Also, the lack of food and
the low pH levels would most likely
kill/deactivate the microorganisms
present (safer for handling).

31. Is the Bio Enzyme alive? In


other words, does it by itself
contain microorganism?
No, by the time the Bio Enzyme is
ready at the end of three months (in
the 100% natural way of producing
with just the three ingredients
mentioned), all the bacteria/yeast that
were working super hard to produce
the Enzyme for you would have died
leaving just the organic compounds
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and proteins behind. There might be


very minor traces of them if you take
them to a microscope but you don’t
need to be concerned about it.

32. Is the Bio Enzyme same as


Fermented Fruit Juice?
No, not at all though the FFJ is made
pretty much the same way but with a
different ratio and different duration.
FFJ is predominantly for gardening
use to aid greater flowering and
fruiting in your plant. You can use the
Bio Enzyme as well in your garden
(in 1: 1000 dilution, mind you) as a
pest control or general well-being
tonic for your plants.
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33. Can I use the Bio Enzymes in


my garden/for my plants?
Yes, you can but in very high dilution.
Also refer the question on fermented
fruit juice above.

34. Can I use the Bio Enzymes in


my composting?
Generally, in composting, we do not
add any liquid as much as
preventable – so while Bio Enzyme
might help in the composting
process, it would not be advisable to
use it any great quantity. You can
use the pulp though – just add the
leftovers to your compost bin to
speeden up the compost!
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35. How come a single product has


such multiple benefits in different
areas?
The high acetic acid concentration
and low pH could be the main
reasons for the many purposes of
GE, as vinegar is well known to be
used a cleaning agent, odor removal,
preventing drain blockages, etc.
Ethanol is known to have antiseptic
properties, while propionic acid is
used in food preservation. These
substances may allow Bio Enzyme to
act as an anti-microbial agent,
insecticide and pesticide. When
diluted, it could provide nutrients to
plants due to the “growth hormones”,
minerals, enzymes and/or other
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organic compounds extracted directly


or converted from the waste
materials. The highlight of Bio
Enzyme is that it is organic and can
be homemade at low costs, as
compared to other products that
contain synthetic chemicals (may be
toxic to human health or
environment) and consume high
energy in their production.

36. Is Bio Enzyme scientifically


analyzed and proven?
No, the scientific community has not
come out with claims either for or
against the Bio Enzyme though there
are a lot of critics that call the Bio
Enzyme nothing more than a glorified
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homemade vinegar. While some tall


claims like "It reduces global
warming" are scoffed at, it has
definitely been found effective in
household cleaning to a great extent.

In essence, Bio Enzymes can help


you save yourself from harsh
chemicals, save money, save waste,
save the clutter of multiple cleaning
liquids in your house, and finally save
the Earth from pollutants and restore
back the balance.

Uses of Bio Enzyme

As with anything else, if the use of


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something is clearly defined, it will


find better and easier adoption with
everyone - same is the case with Bio
Enzyme. While the Internet abounds
with a huge list of how the Bio
Enzyme can be used, there seems to
be no single resource that goes in
depth along with the dilution ratio for
each usage type. So here's a humble
attempt to do that (as I find more
uses/corrections to the notes here on
dilution or other aspects, I will keep
modifying this section).

MEASUREMENT TABLE
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Wait for updates…………

THANK YOU….…
HAVE A HAPPY BIO ENZYME JOURNEY
♥️💗💕💞💓💖❣️♥️
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