1.

Cari gen di gen Bank

>HQ400615.1 Pseudomonas sp. CH-11 plasmid NahAc protein (nahAc) gene,
partial cds
GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCCCCTTCTCTTA
TGGACTATCTCGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACATTCAGGCGGTTTAGAACTGGT
AGGTCCTCCAGGTAAGGTTGTGATCAAGGCCAACTGGAAGGCACCTGCGGAAAACTTTGTGGGTGATGCA
TACCACGTCGGTTGGACGCACGCGTCTTCGCTTCGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCA
ACGCAGTGCTGCCGCCTGAAGGTGCGGGCTTGCAAATGACCTCCAAATACGGCAGCGGTATGGGTGTGTT
GTGGGACGGATATTCAGGCGTGCATAGCGCACACTTGGTTCCGGAATTGATGGCATTCGGCGGCGCTAAG
CAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGCAGCCACCTCAACTGCACCG
TTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGGAACCCGATCGACGCAAACAC
CACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATGCCCGAGGATCTCAAGCGCCGCTTGGCCGAC
GCGGTTCAGCGAACGTTCGGGCCTGCTGGCTTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCAT
CGCAAAACGGCAAAAAATATCAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGT
ATACGGCGACGCGGTCTA

2. Cari gen yang memiliki kemiripan dengan gen tersebut dengan cara klik
“RUN BLAST”,lalu pilih Blast
Hasil dari Blast gen
>HQ400615.1 Pseudomonas sp. CH-11 plasmid NahAc protein (nahAc) gene,
partial cds
GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCCCCTTCTCTTAT
GGACTATCT
CGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACATTCAGGCGGTTTAGAACTGGTAGGTCCTCCAG
GTAAGGTTG
TGATCAAGGCCAACTGGAAGGCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCAC
GCGTCTTCG
CTTCGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAAGGTGCGGGCTT
GCAAATGAC
CTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGATATTCAGGCGTGCATAGCGCACACTTGGTTC
CGGAATTGA
TGGCATTCGGCGGCGCTAAGCAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGC
AGCCACCTC
AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGGAACCCGATCGA
CGCAAACAC
CACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATGCCCGAGGATCTCAAGCGCCGCTTGGCCGACG
CGGTTCAGC
GAACGTTCGGGCCTGCTGGCTTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGC
AAAAAATAT
CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGACGCGGTCTA
>AY048759.1 Pseudomonas fluorescens naphthalene dioxygenase (nahAc)
gene, complete cds
GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCCCCTTCTCTTAT
GGACTATCT
CGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACATTCAGGCGGTTTAGAACTGGTAGGTCCTCCAG
GTAAGGTTG
TGATCAAGGCAAACTGGAAGGCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCAC
GCGTCTTCG
CTTCGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAAGGTGCGGGCTT
GCAAATGAC
CTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGATATTCAGGCGTGCATAGCGCAGACTTGGTTC
CGGAATTGA
TGGCATTCGGCGGCGCTAAGCAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGC
AGCCACCTC
AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGGAACCCGATCGA
CGCAAACAC
CACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATGCCCGAGGATCTCAAGCGCCGCTTGGCCGACG
CGGTTCAGC
GAACGTTCGGGCCTGCTGGCTTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGC
AAAAAATAT
CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGACGCGGTCTA
>AF004283.1 Pseudomonas fluorescens naphthalene dioxygenase ferredoxin
(ndoC1) gene, partial cds, naphthalene dioxygenase iron sulfur protein,
large subunit (ndoC2) gene, complete cds, and naphthalene dioxygenase
iron sulfur protein, small subunit (ndoC3) gene, partial cds
GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCCCCTTCTCTTAT
GGACTATCT
CGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACATTCAGGCGGTTTAGAACTGGTAGGTCCTCCAG
GTAAGGTTG
TGATCAAGGCCAACTGGAAGGCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCAC
GCGTCTTCG
CTTCGCACAGGCGAATCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAAGGTGCGGGCTT
GCAAATGAC
CTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGATATTCAGGCGTGCATAGCGCAGACTTGGTTC
CGGAATTGA
TGGCATTCGGCGGCGCTAAGCAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCCCGCCCGGATTTATCGC
AGCCACCTC
AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGGAACCCGATCGA
CGCAAACAC
CACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATGCCCGAGGATCTCAAGCGCCGCTTGGCCGACG
CGGTTCAGC
GAACGTTCGGGCCTGCTGGCTTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGC
AAAAAATAT
CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGACGCGGTCTA
>AB004059.1 Pseudomonas putida DNA for reductase, ferredoxin, iron
sulfer protein and dehydrogenase, complete cds
GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGCCTGCATCGATCAGGAGGCCCCTTCTCTTAT
GGACTATCT
CGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACATTCAGGCGGTTTAGAACTGGTAGGCCCTCCAG
GTAAGGTTG
TGATCAAGGCCAACTGGAAGGCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCAC
GCGTCTTCG
CTCTGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAAGGTGCGGGCTT
GCAAATGAC
CTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGATATTCAGGCGTGCATAGCGCAGACTTGGTTC
CGGAATTGA
TGGCATTCGGCGGCGCTAAGCAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGC
AGCCACCTC
AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGGAACCCGATCGA
CGCAAACAC
CACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATGCCCGAGGATCTCAAGCGCCGCTTGGCCGACG
CGGTTCAGC
GAACGTTCGGGCCTGCTGGCTTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGC
AAAAAATAT
CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGACGCGGTCTA

3. Sejajarkan sekuenya,pensejajaranya menggunakan clustalomega

Hasilnya adalah

AB004059.1 GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGCCTGCATCGATCAGGAGGCC
HQ400615.1 GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCC
AY048759.1 GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCC
AF004283.1 GAAGTCGCTCGCGTAGAGAGCTTCCATGGGTTCATCTATGGTTGCTTCGATCAGGAGGCC
 **************************************** *** **************

AB004059.1 CCTTCTCTTATGGACTATCTCGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACAT
HQ400615.1 CCTTCTCTTATGGACTATCTCGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACAT
AY048759.1 CCTTCTCTTATGGACTATCTCGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACAT
AF004283.1 CCTTCTCTTATGGACTATCTCGGTGACGCTGCTTGGTACCTGGAACCCATCTTCAAACAT
************************************************************

AB004059.1 TCAGGCGGTTTAGAACTGGTAGGCCCTCCAGGTAAGGTTGTGATCAAGGCCAACTGGAAG
HQ400615.1 TCAGGCGGTTTAGAACTGGTAGGTCCTCCAGGTAAGGTTGTGATCAAGGCCAACTGGAAG
AY048759.1 TCAGGCGGTTTAGAACTGGTAGGTCCTCCAGGTAAGGTTGTGATCAAGGCAAACTGGAAG
AF004283.1 TCAGGCGGTTTAGAACTGGTAGGTCCTCCAGGTAAGGTTGTGATCAAGGCCAACTGGAAG
*********************** ************************** *********

AB004059.1 GCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCACGCGTCTTCG
HQ400615.1 GCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCACGCGTCTTCG
AY048759.1 GCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCACGCGTCTTCG
AF004283.1 GCACCTGCGGAAAACTTTGTGGGTGATGCATACCACGTCGGTTGGACGCACGCGTCTTCG
************************************************************

AB004059.1 CTCTGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAA
HQ400615.1 CTTCGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAA
AY048759.1 CTTCGCACAGGCGAGTCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAA
AF004283.1 CTTCGCACAGGCGAATCTATCTTCTCGTCGCTCGCTGGCAACGCAGTGCTGCCGCCTGAA
** ********** *********************************************

AB004059.1 GGTGCGGGCTTGCAAATGACCTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGA
HQ400615.1 GGTGCGGGCTTGCAAATGACCTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGA
AY048759.1 GGTGCGGGCTTGCAAATGACCTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGA
AF004283.1 GGTGCGGGCTTGCAAATGACCTCCAAATACGGCAGCGGTATGGGTGTGTTGTGGGACGGA
************************************************************

AB004059.1 TATTCAGGCGTGCATAGCGCAGACTTGGTTCCGGAATTGATGGCATTCGGCGGCGCTAAG
HQ400615.1 TATTCAGGCGTGCATAGCGCACACTTGGTTCCGGAATTGATGGCATTCGGCGGCGCTAAG
AY048759.1 TATTCAGGCGTGCATAGCGCAGACTTGGTTCCGGAATTGATGGCATTCGGCGGCGCTAAG
AF004283.1 TATTCAGGCGTGCATAGCGCAGACTTGGTTCCGGAATTGATGGCATTCGGCGGCGCTAAG
********************* **************************************

AB004059.1 CAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGCAGCCACCTC
HQ400615.1 CAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGCAGCCACCTC
AY048759.1 CAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCGCGCCCGGATTTATCGCAGCCACCTC
AF004283.1 CAGGAAAGGCTGAACAAAGAAATTGGCGATGTTCCCGCCCGGATTTATCGCAGCCACCTC
********************************** *************************

AB004059.1 AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGG
HQ400615.1 AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGG
AY048759.1 AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGG
AF004283.1 AACTGCACCGTTTTCCCGAACAACAGCGTGCTGACCTGCTCGGGTGTTTTCAAAGTATGG
************************************************************

AB004059.1 AACCCGATCGACGCAAACACCACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATG
HQ400615.1 AACCCGATCGACGCAAACACCACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATG
AY048759.1 AACCCGATCGACGCAAACACCACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATG
AF004283.1 AACCCGATCGACGCAAACACCACCGAGGTCTGGACCTACGCCATTGTCGAAAAAGACATG
************************************************************

AB004059.1 CCCGAGGATCTCAAGCGCCGCTTGGCCGACGCGGTTCAGCGAACGTTCGGGCCTGCTGGC
HQ400615.1 CCCGAGGATCTCAAGCGCCGCTTGGCCGACGCGGTTCAGCGAACGTTCGGGCCTGCTGGC
AY048759.1 CCCGAGGATCTCAAGCGCCGCTTGGCCGACGCGGTTCAGCGAACGTTCGGGCCTGCTGGC
AF004283.1 CCCGAGGATCTCAAGCGCCGCTTGGCCGACGCGGTTCAGCGAACGTTCGGGCCTGCTGGC
************************************************************

AB004059.1 TTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGCAAAAAATAT
HQ400615.1 TTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGCAAAAAATAT
AY048759.1 TTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGCAAAAAATAT
AF004283.1 TTCTGGGAAAGCGACGACAATGACAATATGGAAACAGCATCGCAAAACGGCAAAAAATAT
************************************************************
AB004059.1 CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGAC
HQ400615.1 CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGAC
AY048759.1 CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGAC
AF004283.1 CAATCCAGAGATAGTGATCTGATTTCCAACCTTGGTTTCGGGAAGGATGTATACGGCGAC
************************************************************

AB004059.1 GCGGTCTA
HQ400615.1 GCGGTCTA
AY048759.1 GCGGTCTA
AF004283.1 GCGGTCTA
********

Primer forward/kiri : 5’GCGTAGAGAGCTTCCATGGG’3 fix

3’ CGCATCTCTCGAAGGTACCC ’5

GC CONTENT 60 %
MELT TEMP 57.7 ºC

Primer reverse/kanan: 5’GCGACGACAATGACAATATGG’3

3’CGCTGCTGTTACTGTTATACC‘5
Hasil oligo analyzer 5'- CCA TAT TGT CAT TGT CGT CGC -3'

GC CONTENT 47.6 %
MELT TEMP 54.1 ºC

TUGAS GEN YANG DITUJU nidA

Bakteri penelitian novousphingobium sp