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Waras Nurcholis1, 2), Laksmi Ambarsari1, 2), Ni Luh Putu Eka Kartika Sari2,
Latifah K Darusman1, 3)
1)
Biopharmaca Research Center, Bogor Agricultural University, Indonesia
2)
Department of Biochemistry, Bogor Agricultural University, Indonesia
3)
Department of Chemistry, Bogor Agricultural University, Indonesia
ABSTRACT
The main bioactive substances in the rhizomes of Curcuma xanthorrhiza and
Curcuma domestica that have eficacy as antioxidant and anti-inflamatory
activities are curcuminoids. In this study, ethanol extracts of C. xanthorrhiza and
C. domestica promising lines from Sukabumi of Indonesia were investigated for
the presence of curcuminoids, antioxidant and anti-inflamatory activities. HPLC
method was used to determined curcuminoids content. The antioxidant (radical
scavenging) potential of the samples was evaluated using 2,2-diphenyl-1-
picrylhydrazyl (DPPH) free radical method. While for the anti-inflamatory
activity, the in vitro cyclooxygenase 2 (COX2) inhibition method was used. The
curcuminoid content of C. xanthorrhiza and C. domestica were 31.27 and 66.32
mg/ g, respectively. IC50 values for DPPH radical scavenging activity were 81.99
and 73.31 µg/ mL, with C. domestica having lowest value and most potent than C.
xanthorrhiza. Percent inhibition values for COX2 inhibitor activity were 74.84
and 67.96 %, with C. domestica having the highest value. In this study, the
ethanol extracts of C. domestica promosing line from Sukabumi of Indonesia
exhibited most in curcuminoids content, antioxidants properties and anti-
inflamatory activity than C. xanthorrhiza promosing line.
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A
C
70.
Curcumin
Curcuminoids content (mg/ g)
60.
50. B
66.32
40.
30.
20. 31.27
Demethoxycurcumin
10.
0.
Curcuma xanthorrhiza Curcuma domestica
Promising lines from Sukabumi
Bisdemethoxycurcumin
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0.8
0.4
0.3
0.2
0.1
0.0
12.5 25 50 100 200
Concentration (µg/ mL)
73.31
80.00 xanthorrhiza. Curcuminoids
compound are the major C.
70.00
xanthorrhiza and C. domestica
60.00 compounds with antioxidant activity
50.00 (Jayaprakasha et al., 2006; Masuda et
al., 1992). This study, we used
40.00
ethanol crude extract of C.
30.00 xanthorrhiza and C. domestica, so
20.00 it’s possible has pure compound
most stronger as DPPH free radical
10.00 scavenging activity than that
0.00 extracts.
C. domestica C. xanthorrhiza
Promising lines from Sukabumi
Anti-inflammatory activity
Figure 3. DPPH free radical Anti-inflammatory activity of C.
scavenging activity of C. xanthorrhiza and C. domestica
xanthorrhiza and C. domestica promising lines from Sukabumi was
promising lines from Sukabumi, evaluated through the percentages of
West Java, Indonesia.
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76 74.84
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