6.

A calibration curve for the colorimetric determination of phosphorous in urine is prepared by reacting
standard solutions of phosphate with molybdenum (VI) and reducing the phosphomolybdic acid
complex to produce the characteristic blue color. The measured absorbance A is plotted against the
concentration of phosphorous. From the following data, determine the linear least-squares line and
calculate the phosphorous concentration in the urine sample: ppm P A 1.00 0.205 2.00 0.410 3.00 0.615
4.00 0.820 Urine sample 0.625

ppm P A
1.00 0.205
2.00 0.410
3.00 0.615
4.00 0.820
Urine sample 0.625

 Slope = 0.205
 Y-intercept = 0
 C = A – 0/0.205
 C = 0.625 – 0/0.205
 C = 3.04 mg/mL