1.

How will you define totipotency - It is the ability of the cell to regenerate complete plant
2. The tissue for the young explants such as hypocotyls easily differentiate somatic embryos because -
It has predetermined embryogenic cells .
3. A species is a -A freely mating group of organisms
4. For the first time somatic embryogenesis was reported in – Carrot
5. How many zones a biosphere reserve should have? - Three zones
6. Which stage of anthers is most responsive for androgenesis - Tetrad stage
7. Which of the followings is the methods to sterilize the heat sensitive compounds - By filtering
through 0.22 microne filter
8. What is the range of pH set for MS medium - 5.5 – 6.0
9. MS medium was originally developed for - Tobacco callus cultures
10. Which of the followings is not a cytokinin – IBA
11. Which of following regeneration system is helpful in getting non-chimeric plants - Direct shoot
organogenesis
12. What is the concentration of ammonium nitrate in MS medium - 1650 mg/l
13. Which of the following plant growth regulators induces senescence? – Ethylene
14. Which of the followings is an example auxotrophic complementation? - Complementation of
nitrate reductase apoenzyme and molybdenum cofactor
15. While testing the viability of the protoplasts, after FDA staining - Live protoplast floured yellow
16. Which of the followings is a method of in situ conservation of germplasm? - Conserving an
organism in National parks
17. Which of the followings is not a cryoprotectant – Lactose
18. Which method of protoplast culture is used if the yield of protoplasts is lower - Hanging droplet
method
19. Which of the following combination of techniques are used for the verification of somatic hybrids?
- Morphology, isozyme pattern, chromosome number and karyotyping
20. Which of the in vitro propagation method is least prone to somaclonal variations - Forced shoot
proliferation
21. protoplasts flotation? - CPW21-S
22. Which of the following metabolic inhibitor combination are used for the selection of somatic
hybrids? - Iodoacetate and diethylpyrocarbamate
23. During selective amplification in AFLP, how many additional random nucleotides are added one of
the primer - 3-4
24. method of longer term conservation? -Seed bank
25. A biosphere reserve can have--------- number of core zones Many
26. Which of the following reactors best suitable for the production of secondary metabolites from
hairy root cultures? - Rotating drum reactor
27. purpose of vetrification in cryopreservation? - To protect tissue from chilling injury
28. How many bands you will get with uni-locus RFLP – Two
29. Which of the followings is used for the induction of secondary metabolite production from plant
cell cultures – Elicitors
30. Which of the following types of culture is most reliable for sustained production of secondary
metabolites? - Hairy root cultures
31. What is the main use of somatic hybridization? - To get plants with genes from distance sources
32. Double standard RNAs are recognized and chopped by which protein – DISER
33. Which of the followings enzyme is not required for the isolation of protoplasts from cell suspension
cultures – Pectinase
34. Which of the followings is not used to slow down culture growth - Reduced light
35. Unstable somaclonal variations arise due to - Epigenetic variations
36. RNAi is used for - Gene silencing
37. Which of the followings is best suitable for the establishment of cell suspension cultures -Friable
callus
38. Which of the followings is the advantage of somatic embryogenesis over shoot organogenesis? - To
get non-chimeric plants
39. For the isolation of protoplasts from single cell culture, which of the followings enzyme is not
required – Pectinase
40. Which of the following primer you will use for RAPD - 5’-CTTACCGATC-3’
41. Cabanicillin is used during agrobacterium mediated transformation for--For the removal of bacteria
42. Which of the vir protein acts as transcriptional factor - Vir G
43. Which of the followings is the most desirable mode of regeneration for getting non-chimeric plants
Indirect somatic embryogenesis
44. Somatic embryogenesis is easy from embryonic explants why? - Embryonic tissue contain more
PEDC
45. Which of the vir gene is constitutively expressed - Vir A
46. Old calli are more prone to somaclonal variants why? - Calli get accumulated polyploid cells
47. What is the role of detergent during mercuric chloride treatment? - For wetting of tissue surface
48. T-DNA complex is formed in - In the cytoplasm of the host cell
49. Phenol chloroform treatment in DNA isolation - For the separation of proteins from nucleic acids
50. At which stage anthers are most responsive for producing haploid embryos - Tetrad stage
51. Why you need to give DPAC treatment for RNA isolation - For the destruction of nucleases
52. Which of the followings is normally more effective for the induction of somatic embryos from leaf
explants - 2,4-D