FEROZSONS LABORATORIES LIMITED Form No.

FZ/210
ANTIBIOTIC EFFICACY TEST REPORT

SOP No. M – 30-01

Product: 707 Hand Sanitizer Liquid
Report No.: 219TC16

Strength: 75 % v/v Batch No.: 1J171 Test Date: 04/01/2017

Method: Serial Dilution & Spread Plate Method Solvent Used: Sterile PO4 Buffer pH - 8.0
Staphylococcus aureus
Stage: Filled Bottle Culture Used:
ATCC 25923
Absorbance Escherichia coli
Wave Length: Culture Used:
ATCC 25922
Turbidity Std. Abs. Bacterial Susp. Abs.
WHO
600 nm Result Date: 05/01/2017
0.116 Abs. 0.119 Abs. Guidelines

Zone of Inhibition Measurements in Millimeters (mm)

Tab-1 Tab-2 Tab-3 Ref. Standard

Sample Zones
28.34 28.18 28.38 27.78
(mm)
28.38 28.2 28.32 27.82

Avg. Zone/Tab. 28.36 28.19 28.35 27.8

Zone Measurement Criteria:

Zone of Antibiotic
Inhibition Disc

Calculations:
Average Sample Zone : Tab-1 + Tab-2 + Tab-3 : 28.3 .
3
Individual %age
P% : Average Sample Zone X 100. Top 102.01%
Standard Zone Mid 101.40%
Bottom 101.98%
: 28.3 X 100.
27.8

%age Assay : 101.8% .

Limit : 90% 110%.

101.80%
Decision : Passed .

Remarks : Released for Coating .

Performed by: Checked by: Approved by:

Microbiologist (Name/Sign./Date) AM Microbiology (Name/Sign./Date) MQC Microbiology (Name/Sign./Date)

Address: P.O. Box Ferozsons Amangarh, Nowshera, KPK, Pakistan.

Phone No: (0923) 614295, Fax No: (0923) 611302