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Gram-Negative Bacilli
Rochelle D. Darlucio, RMT, MPH
Our Lady of Fatima University
College of Medical Laboratory Science
General Characteristics of Nonfermenters
• fail to acidify an oxidative-fermentative (OF) medium when it is
overlaid with __________
• P. aeruginosa
• P. fluorescens Produce pyoverdin
• P. putida •a yellow-green or
• P. veronii yellow-brown
• P. mosselii pigment.
• P. Monteilii
• often produces a weak, slow, positive • Smooth and cream to white colonies in
oxidase reaction. BAP and weakly oxidase (+)
• Almost all strains oxidize glucose, and • The only non-motile among all
many will oxidize maltose, lactose, and pseudomonads
mannitol. • _____________________
• Most strains are lysine decarboxylase and • Causes ________________– infectious
ONPG positive disease of horses, goats, sheep, & donkey.
• most strains are ornithine decarboxylase Rare cause of human infection acquired by
negative and fail to reduce nitrate to direct contact, trauma, or inhalation.
nitrite. • _________– disseminated form of
• motile by means of polar tufts of flagella glander’s disease
• Causes ___________in plants and
________________ in humans
Burkholderia
• Burkholderia pseudomallei • Burkholderia gladioli
• B. diminuta
▫ motile and possess a single polar
flagellum
▫ oxidize glucose
▫ oxidase positive.
▫ Most strains grow on MAC agar.
• B. vesicularis
▫ slender rod, with polar flagella.
▫ Only about 25% grows on MAC agar.
▫ Most strains of produce an
________ intracellular pigment.
▫ oxidase positive and oxidizes glucose
and maltose.
Less Commonly Encountered Nonfermentative,
Gram-Negative Bacilli
• CDC Groups EO-3, EO-4, and • Chromobacterium
Paracoccus
• Chromobacterium violaceum
• oxidase-positive ▫ Motile, facultative anaerobe,
• nonmotile, saccharolytic coccobacilli OXIDASE (+)
that grow weakly, if at all, on MAC ▫ Rare cause of human infection.
agar. ▫ Found in soil and water
• All oxidize glucose and xylose ▫ Unique because of the violet pigment
• EO-3 and many EO-4 isolates have a that it produces – ___________
________________________.