Hypoglycemic Action of Mast Tree

(Polyalthia longifolia) bark Extract in

White Mice (Mus musculus)

An Investigatory Project for

Division Science Fair

Researchers:
Jean E. Javier
Lorennie L. Edera
Jubillee E. Lancin
Jan Mareyne C. Curada
Shane G. Dapon
Blester Orville B. Abis
Jaymar C. Sajol
Andre Rotceh Toledo
Fern Angelo C. Dungo

Research Adviser:
Ludem C. Bacolod

SURIGAO DEL NORTE NATIONAL HIGH SCHOOL

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 Abstract

Polyalthia longifolia is known to be as Mast tree and are commonly found in Brgy. Washington,

Surigao City. The tree has been the subject of quite a few research studies and has been shown to have

properties that could treat diabetes. This study determined the significant effect of Mast Tree (Polyalthia

longifolia) bark extract in reducing the blood sugar level of Albino mice (Mus musculus) as the subject

of the study.

The barks of Mast Tree (Polyalthia longifolia) were collected, boiled with 500 mL water (per

1000 grams of Mast Tree bark) for 15-20 minutes and were filtered. The 225 mL filtrate were divide

based on the following treatment: T1=100% (Cage 1), T2=75% (Cage 2), T3=50% (Cage 3), T4=control

(Cage 4) and was administered to the 9 out of 12 mice as test organisms. The mice were given 0.5 mL

every six hours within one week or seven days after breakfast, lunch and dinner respectively with the

above mentioned treatments.

Data analysis revealed that the decoction obtained from mast tree bark extract had significant

effect in reducing the blood sugar level of mice using 3 different concentrations 100%, 75% and 50%.

Reducing the total blood sugar level showed significant difference from the control set-up from

T1=22.67%, T2= 15.91%, T3=9.95%, T4= -5.83%. Results concluded that the Mast tree (Polyalthia

longifolia) bark had significant effect in lowering the total blood sugar level of albino mice. Therefore, it

can be used as an alternative and effective blood sugar reducer for individuals who had suffered

hyperglycemia ailment.

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 ACKNOWLEGDEMENT

Their companion is to help you achieve your goal, help you to understand, support you morally

and financially. They showed their kind and pleasing personalities. We can’t really make this project by

ourselves.

First of all, the researchers would like to thank Almighty God for giving us knowledge and

wisdom to understand and overcome the challenges ahead of us, for guiding and giving us safety.

Second, we would like to thank Mr. & Mrs. Godofredo T. Javier, Mr. & Mrs. Lorenzo

Edera, Mr. & Mrs. Alexis Lancin, Mr. & Mrs. Gaime Sajol, Mr. & Mrs. Eleuterio Abis, Mr. &

Mrs. Hector Toledo, Mr. & Mrs. Reynaldo A. Curada, Mr. & Mrs. Teodoro . Dapon and Mr. &

Mrs. Fernando Dungo for supporting us financially and morally till the end of this experiment. For

guiding us and being at our back in the hopeless times.

Third, we would like to thank Mr. Godoberto Amora, Ms. Mary Jane Laoguico, Ms. Maridel

L. Zerda, Mrs. Enjelyn C. Gomez, Dr. Life Sheila J. Laugo and Mr. Isaias Elumba for guidance and

for all the knowledge that they have share to us so that we can finish the study well.

Special thanks to Mrs. Ludem C. Bacolod for guidance, support, efforts and teaching us more

about Research II for her faithful and pleasing personality.

To all of you, thank you and may God bless us all!

The Researchers

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 Table of Contents

Tittle Page…….…………………………………………………………………………...….i

Abstract………………………………………………………………………………………ii

Acknowledgement……………………………………….……………………………..…….iii

Table of Contents……………………………………………………………………….……iv-v

Chapter 1 –Introduction………………………………………………………………….….1

A-Background of the Study…………………………………………………………1

B-Statement of the Problem………………….………...………….……….……….2

C- Objectives of The Study……………………………………………………...…2

 Theoretical/Conceptual Framework………………………..………………3

 Assumptions…………………………………………………….……..…...4

C.1 Research Hypothesis…………………………………………………….……..4

D .Significance of the Study………………………………………………..……....4

E. Scope and Limitations…………………………………………..…………..…...5

 Definition of Key Terms………………………………………..………….5-6

F. Review of Related Literature…………………………………………………....7-9

Chapter 2- Methodology………………………………………………………………..…10

A. Materials and Equipment Used…………………………………………….….10

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 B. Treatment and General Procedures…………………………………………11

 Preparation for different concentrations………………………………...11

 Preparation for the Pathogen Free cage…………………………………11

 Selection of Pathogen Free Tested Organism…………………………..11

 Application of different levels of concentration……………………….12

C. Collection of blood sugar before and after treatment……………………….12

 Standard Procedures in Tail Cutting…………………………………12

 Analysis of blood sugar level………………………………………..12

D. Decontamination of Waste disposal………………………………………...13

E. Data Gathering Technique…………………………………………………..13

F. Flow Chart…………………………………………………………………14

G. Research Design …………………………………………………………...15

Chapter 3- Results and discussions……………………………………………..……….16-23

Chapter IV-Summary,Conclusions, Recommendations…………………………….……24

 Summary…………………………………………………………….……24

 Conclusions…………………………………………………………........25

 Recommendations………………………………………………………..26

Bibliography………………...……………………………...…………………………....27-28

APPENDICES……………………………………………………………………………29-31