Professional Documents
Culture Documents
NICU REPORT
Submitted to,
Prof. Mrs. Sonavane M. B.
Principal & HOD child health dept.
INE. Mumbai.
Submitted by, Kedar Vimal
Submitted by
Nagargoje Anita
M.Sc Nursing II year
I.N.E. mumbai
INDEX
1. GENERAL POLICY OF NICU
2. ADMISSION CRITERIA
3. DISCHARGE CRITERIA
5. FLUID MANAGEMENT
6. FEEDING POLICY
7. HYPOGLYCIMEA
2
NICU PHYSICAL LAY OUT
3
KEYS OF PHYSICAL LAYOUT
1. Toilet
3. Nursing station
4. Neonate cubic‟s
5. Isolation cubic
6. Store room
7. Sterilization unit
8. Wash basin
11. Passage
12. Doors
4
RECORDS MAINTAINED IN NICU
1. Weekly indent book
2. Special indent book
3. Surgical indent book
4. Petty supply book
5. Iv fluid book
6. Gauze cotton book
7. Surgical condemn book
8. Stationary book
9. Empty gas cylinder book
10. Biomedical complaint book
11. PWD book
12. Electrician complaint book
13. Servant complaint book
14. X-ray dispatch book
15. Sprit indent book
16. Lab indent book
17. Special indent book
18. Weekly indent book
19. Weekly injection checking book.
20. Admission book
21. Transfer out book
22. Death book
23. Remaining book
24. Night report book
25. Special report book
26. Staff duty register book
27. Drug record book
5
GENERAL POLICY OF NICU
1. INDUCTION
New residents who will join NICU should receive induction from JR III or lecturer
2. DRESSING CODE
3. RECORDS
Need to be dated and timed. The person who makes the entry needs to be signand write
4. HAND WASHING
Air and water sampling should be done only in the case of epidemic.
6
6. EMERGENCY DRUGS AND INSTRUMEENTS
Need to be checked and signed by the senior resident on call and lecturer every morning.
7. OXYGEN CYLINDERS
Full oxygen cylinders are tagged green and empty oxygen cylinders are tagged red
9. REGISTERS
All registers should be coded with green blue or yellow colour and the list of same is
Shown to the care taker by resident doctor/intern before or at the time of discharge
7
ADMISSION CRITERIA TO NICU
1. All baby with weight less than 2kg
6. All baby requiring intervention in form of bag and mask or bag and tube ventilation
11. The same criteria applied to baby not burned at J.J. hospitalexcept that they must
8
Guidelines for Admission to 1. Birth weight less than 1250g.
staffing acuity.
ventilation.
as in (1) apply.
concern.
4. Respiratory problems
9
(b) Any respiratory distress causing concern.
one hour.
5. Gastrointestinal problems
concern.
bowel obstruction.
6. Metabolic problems
feeding.
7. CNS problems
(a) Convulsion.
not ensue.
8. Malformations
9. Cardiovascular
10
unavailable on the postnatal wards.
10. Miscellaneous
Infants that have been born at very low birthweight or low birthweight, represent an at risk group
of children. Increasingly they are being discharged home at a weight less than 2.5kg. The
1. The baby has to be gaining weight – it doesn't have to have reached any particular
11
If breast feeding is being established, it is not a prerequisite that the baby is on full breast
feeding prior to discharge, provided we are happy that the baby is able to suck strongly.
However we must be sure that the mothers are aware of the need to continue to monitor
progress once further feeding changes are made at home, i.e. a switch from
3. The baby must be able to maintain his or her temperature in a cot in a normal household
environmental temperature.
This is particularly important when discharging low birth weight babies home in the
winter.
4. Parents must be willing and happy to take the baby home and to have demonstrated
that they have adequate parenting skills.This may be self-evident, particularly when there
5. Some basic information should be known about the home environment and the
community to which the infant is going, i.e. if they are living in a remote area in a
caravan, then one would be less likely to effect a discharge home at a low birthweight.
up. In remote areas details should be known about the availability of a Well Child Service
visiting. The neonatal home care nurses will be able to provide some initial support and
12
Transfers & Discharges from NICU and PIN
1. For transfers from Level 3 to Level 2 or PIN a formal transfer should occur between
2. Ideally the parents would have been told a few days in advance to accustom to the
3. A transfer letter should be in the notes (and should also be sent to the LMC/GP).
4. The problem list should be up-to-date (as should happen when a discharge/transfer
summary is prepared)
1. Babies transferred to PIN need a formal letter if they have had a complicated course
whilst in Level
2. If they have had a recent transfer summary from Level 3 to Level 2, this may not be
necessary.
1. Babies who are transferred to PIN should have an up-to-date problem summary in
the notes
letter.
3. Babies being transferred to towns outside the Auckland area should have a formal
13
Discharge Documentation
1. A full examination record on the appropriate form (include age in days, weight, length,
head circumference).
Discharge Letters
2. For babies who are being discharged to the postnatal wards and where there are going to
be delays in generating a discharge letter, the baby can be discharged with out a letter
being available. However, it is the responsibility of the resident team (registrar or NS-
ANP) to ensure that a letter is completed as soon as possible and is filed in the notes of
the baby.
3. PLEASE ENSURE that the discharge letter contains the correct information.
1. The database can only use the information available within it.
2. It provides a structure for the letter, and makes an attempt at providing a letter
3. You need to check the letter you have produced and edit it in Word.
4. The quality of the letters you sign your name to reflects on your abilities as well
as the quality of the care that the baby has received in NICU.
5. Please check and edit the letters before you sign them and send them off.
14
ALGORITHM FOR RESUSCITATION OF NEWLY
BORN INFANT
Clear of meconium ?
Breathing or crying? Routine care
YES
Good muscle tone? Provide warmth
Colour pink? Dry the baby
Term gestation? Clear
NO
Provide warmth
Position Clear airway as necessary
Dry stimulate reposition.
Give O2 as necessary
APNEA or HR<100
Provide positive pressure HR > 100
ventilation. Ongoing care
And PINK
HR <60 HR>60
HR<60
Endotracheal intubation may
Administer epinephrine be considered
15
INFECTION CONTROL POLICY IN NICU
Infection prevention is an important component of care of newbornbaby .babies are more
Observing the infection prevention practices below will protect the baby, mother, and health care
provider from infections. This will also help to prevent spread of infection.
2. Consider every person including baby , mother and staff as as potential source of
infection.
3. Through hand washing with soap or alcohol based hand scrub must before and after
5. Use barrier nursing practice whenever indicated such as use of gown , mask and gloves
etc…
8. Routinely clean the newborn special care unit, and dispose of waste immediately.
10. Special precaution while dealing with babies with infection, strict use of barrier nursing.
11. Strict restriction of visitors and health team members with upper respiratory tract
infections
16
BIOMEDICAL WASTE MANAGEMENT AND
HANDLING GUIDELINES
BMW rules were formed under environment protection act, 1986 by the Ministry of
The guideline have been prepared to enable each hospital to implement the said rules.
Further amendments of the Bio medical waste (management and handling) rules 1998
were done by by the government of india in the year of 2000 and 2003.
These rules apply to all person who generate , collect, receive, store , transport,treat,
dispose or handle biomedical waste in any form. Whosoever found breaching these rules
25,000/-.
2. Noscomial infection in patient due to poor infection control and poor Hospital waste
management.
3. Risk of infection outside hospital to waste handlers, scavengers and eventually the
general public.
4. Risk associated with hazardous chemicals, drugs being handled by persons handling
17
Biomedical Waste means any waste , which is generated during the diagnosis, treatment or
SEGRATION OF WASTE:
18
Why is it necessary to segregate waste?
Yellow Bags Human anatomical waste, e.g. human tissues, organs, amputed
Red Bag Soiled dressing & infected plastic tubing‟s, cut syringes, blood
bags.
Red and yellow bags to be taken to designated room near oxygen cylinder between 11:30am -
12:30 pm
Black Bag ( Dry waste) General office waste e.g. papers wrappers etc.
Black bag (Wet waste) Left over food, peels of fruits &vegetables
Used needles tip should be burned in needle burner & tips of syringes should be cut with the
needle cutter.
Use of 1 % hypochlorite for disinfection of needles, syringes, gloves & I.V. Tubing‟s
Card board box Used glass bottles, saline bottles, Packing materials
19
LOCATION OF CONTAINER:
All containers with different color coded bags should be located at the point of generation of
waste, i.e. wards, near OT tables, in injection rooms, diagnostic services areas, dressing trolleys
LABELLING:
All the bags/ containers must be labeled with bio-hazard symbols, according to the rules
Date of transportation.
BAG:
It should be ensured that the waste bags are filled only up to three, fourth capacity, tied securely
and removed from the site of generation to the storage area regularly and thriley.
20
THE QUANTITY OF COLLECTION SHOULD BE DOCUMENTED IN A REGISTER:
STORAGE OF WASTE:
SAFETY MEASURES:
1. Gloves:- disposal gloves, heavy duty rubber gloves, till elbows 9 for cleaners)
4. Plastic aprons
5. Gum boots
6. Any worker reporting with an accident/ injury due to handling of BMW shall be given
prompt aid.
7. All accidental injury should be reported to in charge, should be recorded and should
mention the nature of accident , when & where it occurred, emergency measures taken
21
HYPOGLYCEMIA
Blood glucose to be routinely estimated in following conditions-
6. Infant born to mothers receiving therapy with terbutaline, propranolol and oral
hypoglycemia agents.
7. Infants on IV fluids
8. LGA babies and IDM – sugar to done at 0, 1,2,3,6,12,24, 36, 48 , hours of life.
ASYMPTOMATIC SYMPTOMATIC
Levels > 45- 50 with Levels low with feeds Give bolous 2ml /kg
feeds or doesn’t take feeds of 10 % dextrose
followed by iv
glucose 8 mg /kg/min
22
After nursing IV Glucose sugar to be monitored every 30 minutes. If sugar levels are still
Glucagon 0.025- 0.3mg/kg im (max 1 mg) can be given to hyogycemic infant with good
glycogen stores
hyperinsulemia.
FEEDING POLICY:
1. The optimal care is for all babies to receive breast milk only. This policy addresses those
infants who do not need IV fluids and whose mothers have not established a breast milk
supply.
2. In general, IV infusions should not be started if there are no medical indications for IV
3. Babies who need feeding should be given what mother‟s breast milk is available and
always receive mother‟s breast milk in preference to formula. Be sure to check that no
4. If they require additional feeds, infants should then be started on term infant formula,
after discussion with their mother/father. In such discussions, parents should be informed
that there are few - if any - adverse effects of formula used short term in this way in a
neonatal unit. Click here to review the evidence supporting this policy.
5. For a baby who is already on an IV infusion, it is reasonable to continue the infusion for a
short time if mother‟s milk supply is being established and there is a reasonable
23
expectation that she will be producing enough breast milk with in a day or so. This time
period needs to be judged against the ease of IV access and the condition of the baby.
Babies should not have IVs re-inserted solely because no breast milk is available.
6. Smaller preterm infants will often have a medical indication for ongoing IV fluids and in
them it is desirable to increase the oral fluids slowly. The pace of increase of oral fluids
can usually be matched to the increase in the availability of expressed breast milk.
7. Mothers should be advised and helped with expressing. NICU staff should discuss
expressing as soon as possible. It is accepted that the role of initially helping with
expressing lies with postnatal ward staff. NICU staff should support mothers‟ expression
of breast milk.
8. Nasogastric feeding rather than bottle or cup feeding is advantageous for ex-premature
babies. Term babies who do not have problems with hypoglycaemia can usually
transition directly from IV fluids to breast feeds. Alternatively, bottle or tube feeds may
9. NICU does not provide hydrolysed formula unless there is a clinical indication (other
than a history of allergy). If there is a very strong family history of allergy, hydrolysed
24
EXPRESSION OF THE BREAST MILK
1. Take all aseptic precautions possible.
a. Take a sterile wati, with a warm towel ,wrap the breast for at least 5 minutes.
b. With two finger, using their pulp in circular motions massage breast.
d. Provide massage for atleast 5-10 minutes on each breast before expression of the
milk.
b. Sit comfortably, and hold the sterile container near the breast.
c. Make her put her thumb on her breast above the nipple and areola, and put her
first finger on the breast bellow nipple and areola, opposite to the thumb. And ask
d. Press the thumb and the first finger slightly inward towards the chest wall.
e. Press the breast area behind the nipple and areola ,and than release.
f. Expression of breast atleast for 3-5 minutes until flow slows, then express the
other side.
h. To sustain the milk flow in a mother who has her baby sick , should express every
3 hourly.
25
4. Breast pump can be also used to express the breast milk.
26
WEIGHT RECORDING
2. Assemble all needed supplies and equipment before starting to weigh the baby.
27
28
OXYGEN THERAPY BY HOOD
Oxygen is a gas that the cells in your body need to work properly. The air we breathe normally
Babies with heart or lung problems may need increased amounts of oxygen.
There are several different ways to deliver oxygen to a baby. It depends on how much oxygen is
An oxygen hood is used for babies who can breathe on their own but still need extra oxygen. A
hood is a plastic dome or box with warm, moist oxygen inside. The hood is placed over the
baby's head.
A thin, soft, plastic tube called a nasal cannula may be used instead. This tube has soft prongs
that gently fit into your baby‟s nose. Oxygen flows through the tube. The baby must be able to
Another alternative is a nasal CPAP system. CPAP stands for continuous positive airway
pressure. A CPAP machine delivers oxygen through tubes with soft nasal prongs, but the air is
under higher pressure, which helps the lungs better expand (inflate).
Finally, a breathing machine or ventilator may be needed to deliver increased oxygen to the
baby. The oxygen flows through a tube placed down the baby's windpipe.
29
WHAT ARE THE RISKS OF OXYGEN?
Too much or too little oxygen can be harmful. If the cells in the body receive too little oxygen,
energy production is decreased. With too little energy, cells may not work properly and may die.
Your baby may not grow properly. There is risk for injury to many of the developing organs,
However, too much oxygen can also cause injury. Breathing too much oxygen can cause injury
to the lung. Under certain conditions, too much oxygen in the blood may also lead to problems in
the brain and eye. Babies with certain heart conditions may also require lower levels of oxygen
in the blood. Your baby‟s doctors and nurses try to balance how much oxygen is needed to
minimize the risks of too little or too much oxygen. If you have other questions regarding the
risks and benefits of oxygen in your baby, you should discuss these with your doctor.
Infants receiving oxygen by hood may get cold if the temperature of the oxygen is not warm
enough.
Most (but not all) nasal cannulas use cool, dry oxygen. At higher flow rates, this can lead to
irritation of the inner nose, such as cracked skin, bleeding, or mucous plugs in the nose. This can
Similar problems can occur with nasal CPAP devices. Also, some CPAP devices use wide nasal
30
INSTRUCTION FOR NURSES:
1. Initiate the required flow as confirmed with confirmed using flow meter.
4. Monitor baby for the respiratory rate, saturation, colour and distress.
31
FIXATION OF INTRAVENOUS CANNULA
2. Prior prepare all the required materials like jelco, three way- attached flush, cleaning
5. Assist the physicaian, by holding the extremity proximally and making the vein more
prominent.
6. Once canula is in, help in securing and immobilization of the joint proximal to it with
9. Monitor for extravasation ( swelling and redness), leaking from the canula site, blockage
a. Check and ensure the patency of the cannula with 0.4 ml of normal saline.
c. Administer the drug and reflushnthe cannula to clear the drug from the line.
32
TEMPERATURE MAINTENANCE:
4. Thermometer is cleaned with soap and water, wiped and then used .recatl thermometer
are lubricated with paraffin before use. Insert thermometer 2-3 cm deep it is than
a. Room heaters and over head warmers are used to maintain the desired body
temperature.
c. Actively warm the baby- warm clothes should always be checked for the warmth
33
OVER HEAD WARMER USED IN NICU.
34
ROLE OF NURSE
HEAD NURSE:
1. The Charge Nurse (CN) has shift responsibility within the clinical setting to
ensure the safety and well-being of all patients' on the assigned unit.
assigned departments.
staffing.
6. Takes direct patient care assignment as needed and is held accountable for
8. The CN is clinically involved in daily care activities on the unit and will be
discharges.
35
10. Delegates tasks and duties to appropriate team members in accordance with the
healthcare team members to provide the necessary care for the patients.
STAFF NURSE:
Under general nursing and medical direction, utilizing standard procedures, the Nurse Practitioner or
Physician Assistant functions within an organized system for the delivery of health care in
association with other members of the health care team and performs specialized nurse practitioner
1. Managing patient care of newborns and pediatrics, assisting with the admission assessment
4. Participating in nursing and unit staff meetings and patient care conferences;
36
6. Under general supervision of the Director of Perinatal Nursing, the NICU/Pediatric Nurse
Practitioner or Physician Assistant performs specialized clinical nursing duties and serves as
the clinical expert for the neonatal inpatient population. The essential job functions include
the following:
b. Provides and/or manages the nursing plan of care for neonates with complex
problems;
d. Interprets, coordinates, and implements new and existing policies, methods and
e. Keeps informed of current practices and trends and incorporates them into practice;
institutions/organizations;
37
PEOPLE AS SOURCES OF INFECTION
Sepsis is the most common cause of mortality and morbidity in the nursery. Every hospital
should establish special policy to prevent infection of newborn in baby care area.
Newborn is usually free from harmfull organisms for initial few hours of life. It‟s the hospital
staff or relatives or even the mother tend to spread infection to baby and colonization of
pathogenic organisms.
Prevention of infection is the very cost effective than treatment of neonatal sepsis.
1. Locate the newborn special care unit in a low-traffic area with restricted access.
4. Soap
8. Do not allow staff or visitors to enter the newborn special care unit if they have an acute
38
GUIDELINES FOR ENTRY INTO BABY CARE AREA:
6. Wash the hands with soap and water for 2 minutes following the six steps of hand
3. Mothers are always welcome, attached mother room to baby care area is very convenient.
5. Mothers should be taught , guided and supervised about proper hand washing, good
hygiene.
39
FEEDING POLICY
1. All babies are feed every 2 Hourly either by breast feed, or orogastric tube or by wati
spoon.
3. Sero positive mothers are given option of breast feeding or top feeding and their babies
4. Milk bank support is taken in particular cases like preterm, and cesarian section.
6. Organize feeding: measured length (from nose to ear pinna and to xipoid process) of a
9. Feed the child and let the milk flow with the gravity.
10. Post feeds the ogt is kept closed and the baby put prone with head end at the higher level
11. Wati-spoon feeding: wash hands and collect milk in a sterile wati. Get the baby wrapped
in prewarmed clothing. Hold the baby in semi upright position in lap and feed slowly
with a spoon. Do not pour the feeds in the mouth but let baby suck from the spoon.
40
INITIATION OF KANGAROO MOTHER CARE:
1. Counseling: tell all the benefits of KMC to the mother sand relatives.
6. Baby should be kept naked on the mothers chest in between her breast in a upright
position.
7. Head should be kept turned to one side and in slightly extended position. This allows
9. Babys hip should be flexed and abducted, elbows flexed, with in abdomen lying on the
mother epigastrium. This provides enough room for the babys breathing.
10. KMC actually makes breast feeding easier and holding baby near the breast stimulates
milk production.
11. KMC started earlist as soon as baby is stable. Short KMC is initiated while baby is on
recovery.
12. Each session should be lest be of half an hour or frequent handling may be stressful for
the baby.
13. Session are latter gradually increased only interrupted for the change of diapers.
14. Mother is to relax on easy chairs at an angle 15 degree from the horizontal.
41
IDEAL POSITION OF BABY DURING KMC
42
THE TWO COMPONENTS OF KMC ARE:
i. Skin-to-skin contact
Early, continuous and prolonged skin-to-skin contact between the mother and her baby is
the basic component of KMC. The infant is placed on her mother's chest between the
breasts.
The baby on KMC is breastfed exclusively. Skinto- skin contact promotes lactation and
A mother cannot successfully provide KMC all alone. She would require counseling
along withsupervision from care-providers, and assistance and cooperation from her
family members.
KMC is continued at home after early discharge from the hospital. A regular follow up
and access to health providers for solving problem are crucial to ensure safe and
successfulKMC at home.
BENEFITS OF KMC:
Breastfeeding: Studies have revealed that KMC results in increased breastfeeding rates as well
as increased duration of breastfeeding. Even when initiated late and for a limited time during day
and night, KMC has been shown to exert a beneficial effect on breastfeeding.
43
Thermal control: Prolonged skin-to-skin contact between the mother and her preterm/
LBW infant provides effective thermal control with a reduced risk of hypothermia. For
stable babies, KMC is at least equivalent to conventional care with incubators in terms of
Early discharge:: Studies have shown that KMC cared LBW infants could be discharged
from the hospital earlier than the conventionally managed babies. The babies gained
Less morbidity: Babies receiving KMC have more regular breathing and less
discharge from the hospital, the morbidity amongst babies managed by KMC is less.
44
KMC is associated with reduced incidence of severe illness including pneumonia during
infancy.
Other effects: KMC helps both infants and parents. Mothers are less stressed during
kangaroo care as compared with a baby kept in incubator. Mothers prefer skin-to-skin
contact to conventional care. They report a stronger bonding with the baby, increased
confidence, and a deep satisfaction that they were able to do something special for their
babies. Fathers felt more relaxed, comfortable and better bonded while providing
kangaroo care.
hospital should establish special policy to prevent infection of newborn in baby care
area.
New born is usually free from harmfull organisms for initial few hours of life. It‟s the
hospital staff or relatives or even the mother tend to spread infection to baby and
Prevention of infection is the very cost effective than the treatment of neonatal sepsis.
1. Locate the newborn special care unit in low-traffic area with restricted access.
4. Soap
45
5. Elbow or foot operated taps
8. Do not allow staff or visitors to enter the new born special care unit if they
6. Wash hands with water for 2 minutes following 6 steps of hand washing.
46
HAND WASHING
Hand washing is the simplest and most effective way to of preventing the transmission of
infection and thus reducing the incidence of health care associated infections.
Two minutes hand washing with six steps to be done before entering the unit. 20 seconds
Wash hands with soap and water if they are visibly claen. Disinfect them with using
Instruct the mother and the family members to wash their hands before and after handling
the baby.
2. Wash hands for 10-15 seconds with plain soap and running water.
47
3. Allow hands to air dry or dry them with sterile paper or towel.
4. An alcohol based hand scrub is more effective in cleaning hands than hand
SKIN PREPRATION:
It is the most important step to avoid the entry of pathogenic organisms during any
Again clean it with spirit to wipe of the betadine and let it air dry.
48
Skin is now ready for prick.
49
50
HOUSE KEEPING AND DISINFECTION
and water
Baby linen Wash, dry, autoclave Use autoclave linen each time
Feeding utensils Wash with soap and water, Before each use
dry, autoclave
Injection trays, medicine Wash with soap and water, Daily morning shift
Set of procedure Wash with soap and water, After each use/ every 72 hrs
51
dry, autoclave
Steel drum Wash with soap and water, After use and every 48 hrs.
dry, autoclave
skin thermometer
Rectal thermometer Clean with soap and water After and before each use
and dry
Laryngoscope Blade and handle are after each use remove bulbs
cleaned with spirit swab and cells. Wash with soap and
removed and cleaned with linen put date and time if not
52
AUTOCLAVING
PRECAUTIONS FOR AUTOCLAVING:
Before Use
Attempting to operate an autoclave without proper training from someone familiar with the unit
can cause serious injury or insufficient sterilization of its contents. If training is not an option,
reading the instruction manual and following the manufacturer's directions helps to avoid
Loading
To achieve adequate sterilization, avoid overloading the autoclave, autoclave bags, or containers.
Prevent glass containers from exploding by loosening their caps before placing them in the
autoclave. When loading is complete, securely lock the door to enable a tight seal and avoid
Protective Gear
Protective gear includes insulated gloves, safety goggles, lab coats, rubber aprons, and rubber
boots. Wear insulated gloves when unloading the hot contents of the autoclave or allow the items
to remain inside until cooled. When you open the autoclave door at the end of the sterilization
cycle, wearing rubber boots prevents burns on your feet due to an accumulation of condensed
steam pouring out of the unit. When sterilization is finished, open the autoclave slowly, using the
door as a shield to protect your body from injury due to the sudden release of trapped steam.
53
Glass Precautions
Liquids continue to boil after the sterilization cycle is completed. To avoid fracturing, glass
containers filled with liquid must remain in the autoclave for cooling prior to removal. Carefully
remove glass containers from the sterilization unit to prevent shattering by accidentally hitting
Unloading
Opening the autoclave and the actual physical process of extracting the enclosed materials
presents you with maximum exposure to injury. Your face, hands and arms are more susceptible
to injury from escaping steam and accidental contact with the autoclave walls during this phase
of the sterilization procedure. After the pressure level inside the autoclave registers zero, wait a
few minutes before opening the unit to reduce the potential for burns.
Maintenance
Adherence to proper maintenance of the autoclave is required to insure the safety of you and the
schedule and use technicians qualified to provide this service. Check the efficiency of the
autoclave with sterilization indicator strips that contain live Bacillus spores. By changing color
during the sterilization process, the chemically treated strips verify the death of the spores and
54
STEAM PRESSURE DURING AUTOCLAVING:
A medical autoclave is a device that uses steam to sterilize equipment and other
objects. This means that all bacteria, viruses, fungi, and spores are inactivated.
However, prions, like those associated with Creutzfeldt-Jakob disease, may not be
destroyed by autoclaving at the typical 134 °C for 3 minutes or 121 °C for 15 minutes.
55
OPERATING PROCEDURE:
1. Check the chamber pressure (GAUGE #2 in the Figure) if the autoclave door is already
closed and locked, because a locked door usually indicates that autoclaving is in progress.
Do not attempt to open the door unless the chamber pressure is at ambient. Opening a
chamber filled with pressurized steam can be suicidal. Although some newer models of
autoclaves have built-in safety features to guard against this possibility, never subject
one's own well being to such a shaky assumption. Check the jacket pressure (GAUGE
#1). If the gauge indicates a pressure of between 15-17 psig, proceed to Step 4; otherwise,
3. Slowly open the steam supply valve (VALVE #2). Allow condensate to drain. When the
condensate is completely drained, as evidenced by the flow of steam from the outlet of
VALVE #1, close the condensate valve (VALVE #1). (If pipes start to "bang" hard
during condensate drainage, close VALVE #2 slightly.) Wait for the jacket pressure to
4. Open the door on the autoclave slowly and cautiously. Take out all the items from the
previous autoclaving runs if they are found inside the chamber. Place items to be
autoclaved inside the chamber. Make sure that all the components of each item can
withstand the heat of autoclaving; many plastic materials will melt or deform. Leaving a
few drops of water inside a closed container where the steam in the chamber cannot
easily penetrate will enhance the sterilization effect. If liquid is to be autoclaved, place all
such containers in a stainless steel pan so that any spill can be readily caught. Many
liquids will boil over in the autoclave. The boiling is especially violent when the pressure
56
is released at the end of the autoclave cycle. The use of a pan will also facilitate handling
if more than one container is to be autoclaved. Vent all containers to avoid explosion. If
bottles are capped, make sure that the caps are screwed on lightly so that any excess
pressure can escape. The caps can be screwed tightly later as they are taken out from the
autoclave. At the same time, make sure that everything is closed so that contaminants
cannot enter after autoclaving. Note that the need for pressure relief and prevention of
contaminant entry are not contradictory. For example, shaker flasks can be vented with
cotton seals; fermentor jars, and nutrient jars can be vented with in-line filter units. On
the other hand, some items to be autoclaved cannot be conveniently vented; they must be
placed in a protective steel casing. The glass pH electrodes are such examples.
5. Near the center of the door locking wheel, there is a pin that, when in the inserted
position, prevents the door locking wheel from turning freely. The pin is released from its
hole by pushing the small (approx. 2 inches) lever parallel to the face of the door locking
wheel. To engage the pin, set the lever perpendicular to the face of the door locking
wheel, then turn the wheel slightly to allow the pin to drop into one of the holes intended
for it. Close the autoclave door. Engage the pin, and turn the wheel clockwise by about
1/8 of a turn to insert all the locking bars in the respective slots on the rim of the
autoclave. These bars do not always all slide into the slots at the first attempt. If so,
wiggle the bars as the wheel is turned. After make sure that all the the locking bars extend
securely through the slots, release the pin so that the door locking wheel is now free to
spin. Rotate the wheel clockwise until it is hand-tight; this applies pressure to the door to
seal it air tight so that steam pressure can be built up inside the chamber. Do not over
57
tighten the wheel. Engage the pin to lock the wheel and prevent it from turning back. The
6. Turn the four-position selection valve (VALVE #3) on top of the autoclave to the sterilize
position (STER) by lining up the STER mark with the arrow on the valve. This position
allows the steam to enter the autoclave chamber from the jacket.
7. Wait for the chamber pressure, as indicated by GAUGE #2, to reach the maximum,
where the pressure is to remain stationary during the entire autoclave cycle. The
pressurization of the chamber will take approximately 5 minutes. This marks the start of
the autoclave. The temperature in the chamber can be read with GAUGE #3 at the lower
8. After autoclaving for 20-30 minutes, terminate the autoclaving cycle by shutting off
steam to the chamber and venting it slowly to the ambient pressure. This can be
exhaust position (EXH). It takes about 3-5 minutes to vent the excess steam. It is safe to
open the door only when the needle of the chamber pressure indicator, GAUGE #2, falls
within the narrow white area marked around 0 psig. When the chamber reaches ambient
pressure, release the pin that locks the door locking wheel. Slowly turn the wheel
counterclockwise to relieve pressure on the door. When the door locking wheel is fully
released, engage the pin and turn the wheel 1/8 turn further to disengage the locking bars
from the slots. Open the door slowly. Watch out for a small puff of residual hot steam
being released as the door is first opened. Note that a slight vacuum may be created in the
chamber as the hot air is cooled further while the door is left locked. In this case, a
screwdriver may be used to pry open the autoclave door with care; however this is not
58
recommended for beginning students. Alternatively, introduce steam into the chamber for
a few seconds after securely locking and pressurizing the door will bring back a positive
9. Again, remember to wear gloves. Watch out for the boiling liquid and handle the hot
autoclaved items with great care so as not to burn oneself. Do not touch the hot chamber
wall. Now is the time to make sure that the sterile side of all the sterilized items are not
open to invasion by contaminants. For example, plug the mouth of shaker flasks if the
plugs have come off during autoclaving. Check all connections on a fermentor jar to
make sure that they all remain sealed and that no tubings have been broken. Reconnect
them quickly whenever possible. Remove all autoclaved items from the chamber.
10. Clean up any spills and close the autoclave door when done. Do not pressurize the door.
SUMMARY OF PROCEDURES
8. Clean up.
59
60
NICU ENVIRONMENT:
crucial for the best neonatal outcome. Frequent NICU management procedures like s
imaging are stressful for the baby and also disrupt their sleep [1]. For the very small
preemies, just being handled for daily care, like diaper changing, feeding, vital
should include attention to sound, light, position, touch and other variables. Slevin et
al had shown that significant alteration of the NICU environment for light, noise,
infant handling and staff activity for a specified time period, resulted in reduced
median diastolic blood pressure, mean arterial pressure, neonatal movements and thus
Sound Environment
Loud sound besides being stressful, may lead to loss of hearing. The noise of the incubator
motor is 55-60 decibels (db) and warmer bed, 62 db. The use of mechanical ventilation or CPAP
observation period, 4994 peak noises were recorded. About 90% of these were due to human-
related factors. Loud, sharp sounds can raise noise levels to 100-200 db, which may damage cells
in the ear. This is more likely to happen in sick babies on ototoxic drugs like aminoglycosides.
Loud or sharp sounds can cause physiological changes like tachycardia, tachypnoea, apnoea,
oxygen desaturation and sudden increase in mean arterial blood pressure, disturb sleep, startle
61
the baby and may even produce intracranial haemorrhage in a micropremiee. Sound levels
must be reduced by talking quietly, closing doors and portholes gently, not dropping things on
top of the incubator, turning down machine alarms and phone ring and by modifying staff
soft music transfer love, emotions and wisdom and thus augur long term developmental
Light Environment
Constant light may disturb body rhythm; bright light may not permit baby to open eyes and look
around. Preemies in nurseries with dimmed nightlight, progress more quickly in their sleep-wake
patterns. The amount of light should be reduced by covering the isolettes by laying a blanket
over the top with baby hooked to multisystem monitor. While using phototherapy lights, eyes
must be covered. “Quiet time” must be maintained during the day, when lights are dimmed for
several hours and the baby is not disturbed unless a procedure is really needed. This will help in
62
starting a day/night s l e ep schedul e and suppor t diurna l va r i a t ions in hormone and
temperature levels. Positioning The preemies lack the muscle strength to control movements of
body. They tend to lie with arms and legs straight, or extended, rather than flexed. The
extended position for long periods can lead to abnormal tone with consequent delay in the motor
development. S m a l l p r e e m i e s m a i n t a i n b e t t e r o x y g e n a t i o n ,
temperature, and sleep when they are nursed in prone or lateral positions with mild shoulder
elevation. Sometimes, it is difficult to place the preemie in a curled up, flexed position because
of attachments of lines and sensors. Nesting is one of the key factors in maintaining beneficial
Handling
Handling of preterms may lead to physiologic and behavioural stress, which is shown as
63
and responses like hiccups or yawning. Therefore, minimal handling is the most important
Touch
The sense of touch develops very early in fetal life. In very small preemies, the skin is so fragile
that touching has to be done with great care. For preemies <30 weeks gestational age, studies
show that touch may be stressful rather than soothing. For older preemies, gentle touching can
be helpful. Preemies react in different ways to different kinds of touch. How and how often the
preemie is touched, needs to be based on his responses. A gentle application of bland, sterile oil
is recommended for small preterm neonate. Kangaroo Mother care (KMC) which facilitates
intimate touch of the baby with mother‟s chest and provides various benefits to both mother and
Analgesia
Neonates feel pain as older children and adults. Experiencing pain is stressful with
inevitable, then analgesics like oral sucrose, breast milk, mother‟s nipple, soother, l o c a l a n a
Parental Stress
Having a premature baby is one of the most stressful experiences of a parent. Many aspects of
neonatal intensive care units are stressful to parents, including prolonged hospitalization,
alterations in parenting, exposure to a technical environment, and the appearance of their small,
64
fragile infant [6,7]. Frank, in-depthcommunication with parents is essential, to make them
participate in decision making and babycare. This will remarkably alleviate the parental anxiety
and confusion. Therefore, neonatal care providers should be trained in the art of communication,
in a structured curriculum. Its importance will progressively increase with the enactment of
Effects of light and electromagnetic fields on pineal function could have implications for long-
term risk of breast cancer, reproductive irregularities, or depression in healthcare providers [8].
Therefore, there is need to monitor these variables and develop permissible standards.
Family Involvement
The quality and frequency of family participation in their neonate‟s care in the NICU can play a
significant role in their effectiveness after discharge. Their presence and involvement in the
NICU offer a unique way to humanize the healthcare experience for infants, the i r families
and their caregivers .The salient guidelines for parental involvement are, to teach parents signs of
stress and stability, provide a „parent friendly‟ area to be with their infant, provide privacy for
breast feeding and kparents to use their hands to provide support to the infant and provide
grasping opportunities, support parents in the transition of caring for their preterm infant.angaroo
65
Ethical Environment
NICU is a place where ethical issues are faced very often especially when a neonate suffers from
a nearly fatal condition. It is very difficult to decide to withhold life support or withdraw it. One
has to be very careful in newborn care because consent is given by a surrogate and not by the
patient. It is imperative that NICU should have standard ethical guidelines with full back up of
hospital ethical committee to avoid any ethical catastrophe and shouldpractise detailed
informed consent.
66