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Microtome: Leica Rm2125 Rts & Olympus Microtec Cut 4060 Written Instructions
Microtome: Leica Rm2125 Rts & Olympus Microtec Cut 4060 Written Instructions
School of Medicine
Faculty Health
Prepared By Monique Trengove on 14/06/2017
Megan Ellis
Authorised By Elizabeth Laidlaw – Senior Technical Officer on 19/6/2017
Reviewed By Elizabeth Laidlaw On 19/6/2017
Microtome
Basic operation and safety procedures
The following document identifies the instructions and safety procedures required when using School of
Medicine microtomes - Leica RM2125 RTS (ka2.225) & Olympus microTec CUT 4060 (nb1.115) microtomes.
Description of Leica RM2125 RTS & Olympus microTec CUT 4060 microtomes:
Microtomes are used to cut thin slices of material, known as sections. Microtomes are used in microscopy,
allowing for the preparation of samples for observation under transmitted light or electron radiation.
Preparation of samples:
Please consult appropriate literature for preparation of your samples. Tissue processor and mounting equipment
available in nb1.115 (training required).
Simple Instructions:
• Rotate the handwheel (RHS) until the specimen clamp is in the uppermost position.
• Activate the handwheel lock.
• To insert a specimen into the specimen holder, push the lever forwards.
Specimen Specimen
holder holder
lever Specimen
holder lever
Specimen
holder
Handwheel
Knife holder
Knife holder
clamping lever
• Move the specimen holder to the upper end position by turning the handwheel and engaging the
handwheel lock.
• Release the specimen holder clamp, turn the eccentric lever counterclockwise.
• Turn setscrew #1 to orient the specimen in north-south direction.
• Turn setscrew #2 to orient the specimen in east-west direction.
• To lock the orientation, turn the eccentric lever clockwise.
Eccentric Setscrew
Setscrew
lever #1
#1
Eccentric
lever
Setscrew
Setscrew
#2
#2
5. Trimming the specimen
• Use the coarse feed wheel (left hand side) for a fast horizontal forwards movement of the specimen
– towards the knife – and backwards – away from the knife.
• Once in position, set to a large section thickness (eg. 50uM).
• Bring the specimen closer to the knife by rotating the coarse feed wheel and trim it by
simultaneously rotating the handwheel (right hand side).
• Trim until the desired specimen plane is reached (procedures may vary between microtome
models, please see appropriate manual for detailed instruction).
6. Sectioning
• Set the desired section thickness using the thickness setting knob at the front of the microtome on
the right and check the value in the display window.
Section
Section Thickness thickness
thickness setting
knob
Thickness
setting
knob
• Use a different area of the cutting edge (blade) for trimming than for sectioning. **Use caution
when repositioning blades and always ensure they are clamped back in place.
• For sectioning, turn the handwheel (right hand side) evenly in a clockwise direction. Repeat until
desired number of sections are cut.
• Pick up the sections and mount them on microscope slides by preferred method.
**Always rotate the handwheel at a uniform speed. The rotation speed of the handwheel must be
adapted to suit the hardness of the specimen. For harder specimens, use a slower speed.
7. Changing specimens
• Cover the sectioning edge with the knife guard.
8. Pack up
• Move the specimen clamp to the upper end position and activate the handwheel lock.
• Remove the blade from the knife holder and insert it in the receptacle at the bottom of the dispenser,
or remove the knife from the knife holder and put it back in the knife case.
Safety Precautions
***Lock the handwheel (right hand ride) and cover the knife edge with the knife guard prior to any
manipulation of knife or specimen, prior to changing the specimen block and during all work breaks!***
Always remove the knife / blade before detaching the knife holder from the instrument.
Always put the knives back into the knife case when not in use!
Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife!
SOMHisto
SOM Histology Facility Link Paraffin Embedded Tissue Cutting link