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Reporting of Peripheral smear

Naked eye
 Assess the overall quality of smear
 Whether well prepared
 Edges within the limits of slide
 Well labeled
 Staining quality
Scanner / Low power
 Over all quality of smear
 Distribution of RBC, WBC and Platelets
 Agglutination / Rouleaux formation
 Look for parasites if suspecting
 Select a good area where RBCs just touching
each other
 Put a drop of oil and change to oil immersion
objective.
Be systematic in your study
 First study RBC

 Then WBC

 Finally Platelets
RBC
 Size
 Shape
 Hb content
 Immature cell
 Inclusion bodies
 Intra cellular parasites
Microcytic Hypochromic
Macrocyte
Macrocyte
Poikilocytes
1. Spherocytes
2. Elliptocytes
3. Target cells
4. Sickle cells
5. Fragmented cells- Schistocytes,Helmet cells
6. Acanthocytes
7. Crenated cells
8. Tear drop cells
Spherocyte
Elliptocytes
Target cell
Sickle cell
STOMATOCYTE
Schistocytes
Teardrop cells
Crenated Cells
Acanthocytes
Immature Cells

 Pronormoblast
 Baso.Normoblast
 Poly.Normoblast
 Ortho.Normoblast
 Reticulocyte
 RBC
Polychromasia
Normoblast
Inclusion bodies
 Howel Jolly bodies
 Basophilic stippling
 Pappen heimer bodies
Howel jolly Bodies
Basophilic stippling
Intracellular parasite
WBC
 Count
 Distribution
 Predominant cell
 Immature cell
 Atypical/ Abnormal cell
Dohle
bodies
Immature cell
Macropolycyte
(hypersegmented neutrophil)
Neutrophils with toxic granules
Atypical lymphocyte
Platelets
 Count
 Distribution :Single / Clumps
 Abnormal forms
Reporting
 RBC –size,colour,shape,inclusions,
immature cells,parasites.
 WBC – count,predominant cell,
abnormal cell,immature cell.
 Platelets –count,aggregates
 Extracellular parasites
 Inference.

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