NAMES: Date: 09/23/2021 Score: __

Dalog, Daphne Beth Course & Section: BSN-1A Grp. No.2

Delos Santos, Waveney Frachette Lab. Teacher: Mrs. Nerisa Mantilla

Fabiania, Alexis Marie

Francisco, Estelle Rhine

Francisco, Julianna

Activity No. 4

HYDROLYSIS OF CARBOHYDRATES

A. STARCH

Benedict’s Iodine
TUBE Treatment

Solution’s color varied

A HCl, heat from blue to orange. (+) Alteration of colorless solution into yellow
solution with white precipitate. (-)

Color of the solution

B H O, heat
2 remained blue. (-) Resulting in a mixture with two layers: the top
layer produces a dark violet color and the
bottom layer produces a white color. (+)

From light blue solution to

C RT, H O
2 dark blue solution. (-) Formation of dark blue layers at the bottom of
the mixture. (+)
B. SUCROSE

Benedict’s Seliwanoff’s
TUBE Treatment

Color of the solution was altered Solution’s color changed from colorless
A HCl, heat from blue to orange brown. to maroon/red. Also, red precipitate was
formed. (+)

From blue solution into Red solution was formed with red
B H O, heat
2 mahogany red. precipitate. (+)

The blue solution changed into an Formation of red solution. (+)

C RT, H O 2 aquamarine solution with brown
precipitate.

Question Guide:

1. In which tube (A, B, C) have you observed hydrolysis of starch and sucrose? Prove
your answer.
Starch - Hydrolysis was observed in the starch sample in tube A because a blue-black
color was not seen after the Iodine test which meant that simple sugars were formed
from polysaccharides. The orange product exhibited by tube A after the Benedict's test
also proved that reducing sugars are present and that starch was reduced to
monosaccharides.

Sucrose - All sucrose samples in the three tubes underwent hydrolysis. In Benedict's
test, tube A formed an orange brown, tube B showed a mahogany red color, and tube C
had an aquamarine result, which means that all three samples had traces of
monosaccharides in them. Seliwanoff's test also resulted in the formation of a red color
in all three samples indicating that there is a presence of fructose.

2. Write the equation involved in the hydrolysis of sucrose.

The hydrolysis of sucrose (C H O ) produces glucose and fructose, which the two
12 22 11

sugars have the same formula (C H O ) but different form (which glucose is aldose
6 12 6

while fructose is ketose).

Step 1

C H O —> C H O + C H O
12 22 11 6 12 6 6 10 5

(Fructose)
Step 2

C H O —> H O + C H O
6 10 5 2 6 12 6

(Glucose)

3. Give the stepwise hydrolysis of starch and its reaction to benedict’s and iodine
test.

POLYSACCHARIDE

Starch

DISACCHARIDES

Sucrose Maltose Lactose

MONOSACCHARIDES

1 glucose 2 glucose 1 glucose

1 fructose 1 galactose

POLYSACCHARIDE
Glycogen