Purpose Sub Category Gene Primer

91E _F:
Universal Bacteria 16s rDNA
13B_R:
TStaG422
Staphylococcus spp Tuf
TStag765
756F
16s rDNA
Staph-750R
S. aureus
au-F3
nuc
au-nucR
spa-F
spa typing spa
spa-R
in-F
S. intermedius
in-R3
sch-F
S. schleiferi
sch-R
dea-F
S. delphini A
dea-R
hy-F1
S. hyicus
hy-R1
pse-F2
S. pseudintermedius
pse-R5
deb-F
S. delphini B
deb-R4
Detection

fblF
S. lugdunensis fbl
fblR
sapF
S. saprophyticus sap
sapR
mvaA1 (hae1)
S. haemolyticus mvaA
mvaA2 (hae2)
sepF
S. epidermidis sep
sepR
homF
S. hominis hom
homR
d1
Enterococcus spp sodA
d2
FL1
E. faecalis sodA
FL2
FM1
E. facium sodA
FM2
CE1
E. cecorum sodA
CE2
GA1
E. gallinarum sodA
GA2
HI1
E. hirae sodA
HI2
CA1
E. casseliflavus sodA
CA2
TEcol553
E. coli & Shigella tuf
 E. coli & Shigella tuf
TEcol754
ECA75F
E. coli 16s rDNA
ECR619R
stx1_F
stx1
stx1_R
EHEC
stx2_F
stx2
stx2_R
Detection + Virulence

eae_F
eae
eae_R
EPEC
bfpA_F
bfpA
bfpA_R
aggR_F
EAEC aggR
aggR_R
elt_F
elt
elt_R
esth_F
ETEC esth
esth_R
astp_F
astp
astp_R
invE_F
EIEC invE
invE_R
astA_F
ETEC, EAEC astA
astA_R
PA 6
B. anthtracis pag
PA 7
Salmonella invA

SAZZAD primers

ALOMGIR primers

luk-PV-1F
S. aureus virulence PVL
luk-PV-2R
asa1_F
asa1
asa1_R
gelE_F
gelE
gelE_R
cylA_F
cylA
cylA_R
Virulence

esp_F
esp
esp_R
lyl_F
lyl
lyl_R
Enterococcus spp Virulence
ace_F
ace
ace_R
efaA_F
efa
efaA_R
Vi
fsrB_F
fsrB
fsrB_R
agg_F
agg
agg_R
cpd_F
cpd
cpd_R

SAZZAD primers

ALOMGIR primers

mecA-1F
Methicillin resistance mecA
mecA-2F
ileS2F (mup1)
Mupirocin resistance leS2
ileS2R (mup2)
aac F
Glycopeptides Resistance (6 )Ie-aph(2 )Ia R
AMR

vanA_F
vanA
vanA_R
vanB_F
vanB
vanB_R
Vancomycin Resistance
vanC1_F
vanC1
vanC1_R
vanC2/C3_F
vanC2/C3
vanC2/C3_R

SAZZAD primers

ALOMGIR primers
 Sequence(5'--3') Primer dilution date Reference
GGAATTCAAAKGAATTGACGGGGGC
CGGGATCCCAGGCCCGGGAACGTATTCAC
GGC CGT GTT GAA CGT GGT CAA ATC A
F. Martineau et al. 2
TIA CCA TTT CAG TAC CTT CTG GTA A
AAC TCT GTT ATT AGG GAA GAA CA
CCA CCT TCC TCC GGT TTG TCA CC
TCGCTTGCTATGATTGTGG
GCCAATGTTCTACCATAGC
GACGATCCTTCAGTGAGCAAAG
GCAGCAATTTTGTCAGCAGTAG
CATGTCATATTATTGCGAATGA
AGGACCATCACCATTGACATATTGAAACC
AATGGCTACAATGATAATCACTAA
CATATCTGTCTTTCGGCGCG
TGAAGGCATATTGTAGAACAA
CGRTACTTTTCGTTAGGTCG
CATTATATGATTTGAACGTG
GAATCAATATCGTAAAGTTGC
TRGGCAGTAGGATTCGTTAA
CTTTTGTGCTYCMTTTTGG
GGAAGRTTCGTTTTTCCTAGAC
TATGCGATTCAAGAACTGA
AAATCTCCAAGTTGACCAAACATAC
GATTGCGCTGAAAGAATTGC
AACGGGCGTCTCGATAGAAAA
AACGGGCGTCCACAAAATCA
GGT CGC TTA GTC GGA ACA AT
CAC GAG CAA TCT CAT CAC CT
CAG TTA TAC GGT ATG AGA GC
CTG TAG AGT GAC AGT TTG GT
TAC AGG GCC ATT TAA AGA CG
GTT TCT GGT GTA TCA ACA CC
CCITAYICITAYGAYGCIYTIGARCC
ARRTARTAIGCRTGYTCCCAIACRTC
ACTTATGTGACTAACTTAACC
TAATGGTGAATCTTGGTTTGG
GAAAAAACAATAGAAGAATTAT
TGCTTTTTTGAATTCTTCTTTA
AAACATCATAAAACCTATTTA
AATGGTGAATCTTGGTTCGCA
TTACTTGCTGATTTTGATTCG
TGAATTCTTCTTTGAAATCAG
CTTTCTGATATGGATGCTGTC
TAAATTCTTCCTTAAATGTTG
TCCTGAATTAGGTGAAAAAAC
GCTAGTTTACCGTCTTTAACG
TGGGAAGCGAAAATCCTG
CAGTACAGGTAGACTTCTG
GGAAGAAGCTTGCTTCTTTGCTGAC
AGCCCGGGGATTTCACATCTGACTTA
AGTTAATGTGGTGGCGAAGG
CACCAGACAATGTAACCG
TTCGGTATCCTATTCCCGG
CGTCATCGTATACACAGGAG
CCCGAATTCGGCACAAGCATAAGC
CCCGGATCCGTCTCGCCAGTATTCG
AATGGTGCTTGCGCTTGCTGC
GCCGCTTTATCCAACCTGGTA
GTATACACAAAAGAAGGAAGC
ACAGAATCGTCAGCATCAGC
AACGTTCCGGAGGTCTTATG
CAACCTTGTGGTGCATGATG
TTCACCTTTCCCTCAGGATG
ATAGCACCCGGTACAAGCAG
ACTGAATCACTTGACTCTTCA
TCACAGCAGTAAAATGTGTTGT
GCAGGAGCAGATCTTGAAG
GAAAGGCACGAGTGACTTTC
CCATCAACACAGTATATCCG
ACGGCTTTGTAGTCCTTCCA
ACCAATATCAAAGAACGACGC
ATCACCAGAGGCAAGACACCC

ATC ATT AGG TAA AAT GTC TGG ACA TGA TCC A
GCA TCA AGT GTA TTG GAT AGC AAA AGC
GCACGCTATTACGAACTATGA
TAAGAAAGAACATCACCACGA
TATGACAATGCTTTTTGGGAT
AGATGCACCCGAAATAATATA
ACTCGGGGATTGATAGGC
GCTGCTAAAGCTGCGCTT
AGATTTCATCTTTGATTCTTGG
AATTGATTCTTTAGCATCTGG
ACAGAAGAGCTGCAGGAAATG
GACTGACGTCCAAGTTTCCAA
GGAATGACCGAGAACGATGGC
GCTTGATGTTGGCCTGCTTCCG
GCCAATTGGGACAGACCCTC
CGCCTTCTGTTCCTTCTTTGGC
ATGCTAATCGATTGGATTCTAAAA
TCTTTTTAGGTTTTTCAGTTTGTC
AAGAAAAAGAAGGTAGACCAAC
AAACGGCAAGACAAGTAAATA
TGGTGGGTTATTTTTCAATTC
TACGGCCCCTCTGGCTTACTA

GTA GAA ATG ACT GAA CGT CCG ATA A

CCA ATT CCA CAT TGT TTC GGT CTA A
TAT ATT ATG CGA TGG AAG GTT GG
AAT AAA ATC AGC TGG AAA GTG TTG
GAGCAATAAGGGCATACCAAAAATC
CCGTGCATTTGTCTTAAAAAACTGG
GCTATTCAG CTGTACTC
CAGCGGCCATCATACGG
CATCGCCGTCCCCGAATTTCAAA
GATGCGGAAGA TACCGTGGCT
GGTATCAAGGAAACCTC
CTTCCGCCATCATAGCT
CTCC TACGATTCTCTTG
CGAGCAAGACCTTTAAG