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In this way cloning can supply the large amounts of DNA needed
for molecular biological studies of gene structure and expression
(b) This gene is disrupted when new DNA is inserted into the vector;
as a result the recombinant host does not display the relevant
characteristic.
Insertional inactivation
of tetracyclin R gene
Replica plating
Other than antibiotics there are several system that targets
different genes
fungi,
animals,
plants,
human
Transformation of individual cells
Liposomes
Protoplasts
Electroporation
Microinjection,
Biolistics
Microinjection
Biolistics