Professional Documents
Culture Documents
and Biophysics
of health professionals
ASKLEPIOS 2013
ISBN 978–80–7167–177–0
Digital study text "Basics of Medical Physics and Biophysics for electronic
education of health professionals" has been supported by the grant project KEGA
052UK-4/2013 offered by The Ministry of Education, Science, Research and Sport
of the Slovak Republic.
The digital study text has been supported by the GP MŠSR KEGA 052UK-4/2013.
No part of this text may be reproduced, saved, copied or replaced electronically,
mechanically, photografically or by other means without written agreement of
authors and publisher.
ISBN 978–80–7167–177–0
Basics of Medical Physics and Biophysics for electronic education of health professionals
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CONTENTS Page
Introduction 7
1. Cell biophysics I. 14
1. 1. The properties of biological fluids
and transport features through cell membrane (Herdová) 14
1.1.1. Surface tension 15
1.1.2. Viscosity 16
1.1.3. Other important properties of fluids 19
1.1.4. pH-metry 19
1.1.5. Diffusion 21
1.1.5.1. Biophysical basis of pharmacokinetics (Miklovičová) 22
1.1.5.2. Experimental methods to study diffusion 26
1.1.6. Osmosis and osmotic pressure 27
1.1.7. Transport processes on biomembranes 31
1.1.7.1. Structure of biological membranes 31
1.1.7.2. Transport processes through the biological membrane.. 32
1.1.8. Dialysis therapeutic methods (Herdová) 33
1.1.8.1. Hemodialysis (Kukurová) 34
1.1.8.2. Peritoneal dialysis 34
1.1.8.3. Hemofiltration 35
1.1.8.4. Hemoperfusion 35
1.1.9. Optical methods of measuring solute concentra¬tions (Kukurová) 36
1.2. Cell biophysics II. 40
Electrical properties of cells and organs (Herdová) 40
1.2.1. The resting potential. Its origin and properties 40
1.2.2. Origin and propagation of the action potential (AP)
on the membrane (Hochmannová) 41
1.2.3.Methods of measuring electrical properties of cells
and tissues (Hochmannová) 44
1.2.3.1. Measurement of AP using the internal microelectrode method 44
1.2.3.2. Double sucrose gap and vaseline gap methods 45
1.2.3.3. The patch clamp method 47
1.2.3.4. Measurement of action potentials in peripheral nerves 48
1.2.4. Modeling of the electrical properties of cells (Herdová) 49
1.2.4.1. Model of AP generation on the surface membrane of a muscle cell 49
1.2.4.2.Model of summation of excitations on the neuron input 50
1.2.5. Recording and processing heart action potentials 51
1.2.5.1. Electrocardiography (Kráľová) 51
1.2.5.2. Computer methods of processing biosignals (Kukurová) 56
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Title: Basics of Medical Physics and Biophysics for electronic education
of health professionals
ISBN 978–80–7167–177–0