CHM043.

DATE: Oct 19, 2021

NAME: Maranda Ryan P. SIGNATURE: __________________

QUALITATIVE TESTS FOR CARBOHYDRATES

A . Solubility Test
Substance
Tested Results
Soluble in water

Glucose

Soluble in water

Fructose

Soluble in water

Sucrose

Soluble in water

Lactose

Insoluble in water

Starch
B. Molisch Test
Substance
Tested Description of Visible Results
Glucose Purple- Colored Dye

Fructose Purple- Colored Dye

Sucrose Purple- Colored Dye

Lactose Purple- Colored Dye

Starch Purple- Colored Dye

C. Seliwanoff’s Test
Substance Time
(in secs) or
Tested Result
rank with
respect to
formation of
solid
Glucose 66 secs Positive Control

Fructose 66 secs Positive Control

Sucrose 66 secs Positive Control

Lactose 66 secs Positive Control

Starch 66 secs Positive Control

D. Benedict’s Test

Substance Tested Result

Formed red precipitate
Glucose
Formed red precipitate
Fructose
Formed red precipitate
Lactose
No red precipitate formed
Sucrose
No red precipitate formed
Starch

E. Barfoed’s Test

Substance Tested Result

Brick red precipitate formed
Glucose
Brick red precipitate formed
Fructose
Brick red precipitate formed in prolonged in heating
Lactose
Scantly brick red precipitate formed if concentration is 1%
Sucrose
F. Fehlings A and B Test
Substance
Tested Description of Visible Results
Glucose Formed red percipitate

Fructose Formed red percipitate

Sucrose Formed red percipitate

Lactose No red precipitate formed

Starch No red precipitate formed

G. Iodine Solution
Substance
Tested Description of Visible Results
Positive in the iodine test

Glucose

Positive in the iodine test

Fructose

Positive in the iodine test

Sucrose

Positive in the iodine test

Lactose

Positive in the iodine test

Starch
Questions:

1. Will disaccharides and polysaccharides give a positive result for Molisch test?
All the carbohydrates such as disaccharides and polysaccharides give a positive
result on the Molisch test.
2. Can Seliwanoff’s test be used to distinguish sucrose from fructose? Explain your
answer.
Seliwanoff’s test is a chemical test which distinguishes between aldose and
ketose sugars.so yes it can distinguish the sucrose and fructose if it is a ketose
sugar like fructose and sucrose is the solution turns cherry red. If it is an aldose
sugar, then a faint pink color may appear.

3. Which of the carbohydrates used is/are?

a. Monosaccharide? Glucose, Fructose
b. Disaccharide? Sucrose, lactose
c. Polysaccharide? Starch
d. Ketohexose? Fructose
e. Aldohexose? Glucose
4. Which solution did not react with Benedict’s test?
The Sucrose and the starch did not react on the Benedicts test.

5. Explain why Fehlings’s A is coupled with Fehlings B. (please do research)

Fehling's solution is prepared by combining two separate solutions: Fehling's A, which is

a deep blue aqueous solution of copper (II) sulfate, and Fehling's B, which is a colorless
solution of aqueous potassium sodium tartrate (also known as Rochelle salt) Strongly
made alkali with sodium hydroxide. These two solutions, stable separately, are
combined when needed for the test because the copper (II) complex formed by their
combination is not stable: it slowly decomposes into copper hydroxide in the alkaline
conditions.