Fullerene, Super Anti-Oxidant

-Clinical Test Results on Whitening and

Anti-Aging-

Vitamin C60 Bioresearch Corporation

Tomoko OHTSU
August 30th ,
2013
Minimum Knowledge about Fullerene!
1. Fullerene(C60) is a carbon allotrope of carbon, like a diamond.

2. Fullerene(C60) is a Nobel Prize winning material

3. Fullerene(C60) is a super anti-oxidant.

- 172 times as effective as Vitamin C

- stable against light and/or heat
- power is long-lasting (more than 24 hours)

2
 Great Effects of Fullerene

Anti-aging（preventive effects of cell death)

Whitening Sunscreen / UV cut

（Melanin Control）

Pore Tightening
Inflammation Easing
(reducing of a ruddy face)
Recover
Skin barrier
Anti-Wrinkle
Sebum Oxidation Control
3
 Data of Fullerene Effect
- in vitro Study-
1. Free Radical Scavenging activity

2. Comparison data with

other anti-oxidant
ingredients

3. Preventive Effect of Fullerene

4
Free Radical Scavenging activity

high

Concentration of Free
Radical
low

Without Fullerene With Fullerene

5
Radical scavenging activity of Fullerene
100
Antioxidant Activity (AOA%)

80

60

40

20

Linoleic acid H2O2 UV ray

-20

Vitamin E Vitamin C Derivative(APS) Fullerene(Radical Sponge)

6
 Antioxidant Activity(AOA%)
Antioxidant
Activity(AOA%)

20
60

40

0
PVP/C60

PVP/C70 49.5

PVP/C60O 35.6

PVP/C60On
29.1

vitamin E

catechin
25.1

isoflavone
17.7

coenzyme Q10

quercetin
14.8

crucmin
ingredients by β-Catotene method

APPS
7.35
Comparative testing with other antioxidant

a-lipoic acid
7
Preventive effect of Fullerene
Without Fullerene With
Fullerene

11 hours later
8
Clinical Test Results by using Fullerene
contained cosmetics

1. Brightening (Whitening) Effects

(Radical Sponge™ )

2. Effect of Fullerene on the Skin Barrier

(Radical Sponge™)
3. Fullerene is effective against wrinkle
( LipoFullerene™ )

9
 Brightening (Whitening) Effects
by Fullerene

Effectively eliminate UV-induced free

radicals

Prevent excessive melanin production

Verified by various clinical trials

10
Suppression of Melanin production by Fullerene

Without Fullerene With Fullerene

Let’s check the movie!!

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 Brightening (Whitening) Effect by Fullerene
Subjects: 18 females aged 34 to 49 Area: Face
The melanin index decreased intentionally on the 4 weeks.

1.3 p < 0.001

p < 0.001
1.2 p < 0.001
1.5
1.1
1.0

Hemoglobin Index
Melanin Index

0.9
1
0.8
0.7
0.6
0.5

0
0 4 8 (weeks） 0 4 8 （weeks）

You can feel the Whitening/Brightening effect in about 1-2 month.

Continuous use of Fullerene contained product is the key to have better
results. 13
Brightening (Whitening) Effect by Fullerene

After 3 month

After 5 month

Continuous use of Fullerene contained product will lead you

more brighter and fair skin condition.
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 Effect of Fullerene on the Skin Barrier
by Fullerene

Increase “Transglutaminase 1” mRNA

expression.

Increase “Cornified Emvelope” formation and

help maintain Skin barrier function.

Clinical study: Help in recovering skin barrier

function. 15
 Structure and function of the stratum corneum

Cornified Intercellular lipid Moisture loss

envelope
（CE）
dry

corneum
Stratum
skin

20 μm
Cornified
dullnes
cell
s

rough
skin
Granular layer
・drying flecks
・UV wrinkles
・stress
・aging
Normal stratum corneum Stratum corneum with decreased
skin barrier function

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Clinical Test :
Tape Stripping Method to measure Trans Epidermal Water
Loss (TEWL)
Radical Sponge® helped recovery of TEWL on 2nd and 3rd day
significantly
100 RS(0% RS(1%)
)
80 #
Rate of recovery （％）

60 #P<0.05 vs. RS(0%) #

40

20

0
days
-20

-40 0 1 2 3
Trial Design
40 1 2
Randomized 3 study
blinded 4
Method TEWL changes after Tape Stripping

Subjects 10 healthy subjects (5male 5female)

Trail period and applications Solution was applied twice daily for 5days
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Fullerene is effective against wrinkle
by Fullerene

Remove the Free Radicals which is the

primary cause of wrinkles.

Skin structure is improved steadily.

Fullerene/squalene cream significantly

reduced wrinkle area compared to the
placebo-treated area.

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 Wrinkles formation by ultraviolet rays
Decrease of MMP9 and FOS mRNA means repression of wrinkles formati

The active oxygen which occurred by ultraviolet rays activates FOS

(intracellular signaling) and MMP9 (degradative enzyme of collagen),
and forms wrinkles.

Wrinkle
UVB formation
RO
S
Epidermi
s FOS
laminin-5
Basement
membrane IV type collagen

Dermis
VII type collagen

MMP9（Matrix metallopeptidase 9）・・・ degradative enzyme of laminin-5 etc. 19

 Fullerene is effective against wrinkle
by LipoFullerene
Guidelines for Evaluation of Anti-
Guideline Wrinkle Products prepared by the
s Japanese Cosmetics Science Society
in 2006
Trial
A randomized, double-blind test
Design
Trial Eight weeks (September 24, 2008 ～
Period November 19th, 2008）
23 Japanese women in their 30s to
Subjects
40s with wrinkle grade 2 to 3.
1%-LipoFullerene-blended cream
Test
(active) and Lipofullerene free cream
Samples
(placebo)
Evaluation Skin surface replica Shadow image

10 x 10 mm
Wrinkle: few many

The percentage of wrinkle area decreases in the

20
LipoFullerene application group than a Placebo application
 Great Effects of Fullerene

Anti-aging（preventive effects of cell death)

Whitening Sunscreen / UV cut

（Melanin Control）

Pores Tighten
Inflammation Easing
(reducing of a ruddy face)
Moisturizer
(Skin barrier)
Anti-Wrinkle
Sebum Oxidation Control
21
 For Basic Skin Care Products and MORE!
1. Color Cosmetics
Basic Skin Care Products (BB Cream, CC Cream)
- Lotion
- Essence/Serum
- Cream 2. Hair care
(Scalp tonic,
Shampoo & Treatment)

+ 3. Beauty Laser care

4. Sun screen

5. Facial Mask

22
Fullerene Products in
Overseas

Anti-oxidant Whitening Serum

With Fullerene RS™

23
Fullerene is famous for Doctor’s Cosmetics
More than 1,500 clinics in Japan
has selected our Fullerene cosmetic
ingredients!!
1600 Number of Clinics
1400
1200
1000
800
600
400
200
0
2005 2007 2009 2011 2013

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Fullerene Contained Products
St.Clare
（Taiwan）
TUNEMAKERS

WithUs
アプロ
NOVA C60
（Canada）
2011 Over 1000 items
Dr.WU サイエンス are selling in the
MISSHA
（Taiwan） （Korea） market !!
JMEC

Unicity アジュバン Felice Towako

コスメ Dr. Recella Cosmetics
2009
AR
Launch LipoFullerene
CBON
Dr.Perricone
（USA） DHC
Dr.Ci labo 2008
Oil-Soluble
Doctor Products 1000 Clinics selected Fullerene Fullerene
Dr.Brandt
ISA Nox （USA） 2007 Entered to Internet Market
(Korea）
マードゥレクス 2006 Start Selling in Overseas（Korea・Taiwan・USA）
Derfarma

For Clinical
Doctors
Water-Soluble
2005 Launch Radial Sponge Fullerene 25
Our Products

Radical Sponge LipoFullerene

(water-soluble)
(oil-soluble)

26
 Trademark (LOGO)

Products which containing more than 1% of our ingredients(Radical Sponge

or LipoFullerene) can put our trademark to their product package and web p
27
Marketing Points of Fullerene!!

Nobel Prized Winning Ingredient

172 times as effective as Vitamin C

Stable against light(UV) and/or heat

Power is long-lasting (more than 24 hours)

Abundance of Clinical data

Multifunctional ingredient

Safety and secure ingredient selected by Clinic

Doctors 28
Samples of our ingredient is available!!

After concluding Non-Disclosure Agreement (Sample

Evaluation Agreement), VC60 will send sample to the
customer for free.

• Radical Sponge (water-soluble Fullerene) : 20 g

• LipoFullerene (oil-soluble Fullerene): 16 g

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Sample formulations are available!!

• Radical Sponge (water-soluble Fullerene)

Serum & Cream

• LipoFullerene (oil-soluble Fullerene):

16 g

Milk Lotion W/O type & O/W type

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Exhibitions

in-cosmetics Asia 2013

Date: October 29th – 31st, 2013

Time: 10:00 – 17:00
Place: BITEC Hall Bangkok,
Thailand
Booth number: E10
(Booth share with Bronson &
Jacobs)

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Thank you for your attention!

Vitamin C60 BioResarch Corporation

Contact:
info@vc60.com
+81-3-3517-3252

http://www.vc60.com