Chromatographic Techniques

It is a type of COLUMN Definition:

chromatography in which CHROMATOGRAPHY (CC)
the mobile phase is a It is an important
carrier gas, usually an It is a method used to biophysical technique
inert gas such as helium purify individual chemical that enables the
or an unreactive gas such compounds from separation, identification,
as nitrogen, and the mixtures of compounds. and purification of the
stationary phase is a components of a mixture
microscopic layer of for qualitative and
liquid or polymer on an quantitative analysis.
inert solid support a
mostly used
THIN LAYER
CHROMATOGRAP
HY (TLC)
Classification of
(TLC) is a mostly used Chromatographic
technique which involves Techniques:
a stationary phase of a
thin layer of adsorbent
like alumina on a flat,
HIGH PERFORMANCE inert
LIQUID layer of substrate.
CHROMATOGRAPHY
(HPLC)
High-performance liquid
chromatography (HPLC) is
a form of column Chromatographic
chromatography used
Techniques:
frequently in
biochemistry and
analytical chemistry.

FAST PROTEIN LIQUID

CHROMATOGRAPHY
GAS – LIQUID
usually referred to as
FPLC, is a form of column CHROMATOGRAPHY
chromatography used to (GLC)
other chromatographic
methods apply.
COUNTER-CURRENT
CHROMATOGRAPHY
CCC or Partition
Chromatography, in
general is used to
separate components of
a mixture based on their
differing affinities for
mobile and stationary
phases of a column.
DISPLACEMENT separate or purify
CHROMATOGRAPHY proteins from complex
mixtures. It is very
is a preparative commonly used in
technique in which a biochemistry and
sample is placed onto the enzymology.
head of the column and is
then displaced by a
solute that is more
strongly adsorbed than
the components of the
original mixture.
GAS CHROMATOGRAPHY
(GC) is commonly used in
analytical chemistry for
separating and analyzing
compounds that can be SUPERCRITICAL FLUID
vaporized without CHROMATOGRAPHY
decomposition.
(SFC)
It is a robust and easy-to-
use form of normal phase
chromatography ideally
suited to the analysis and
AFFINITY purification of low to
CHROMATOGRAPHY moderate molecular
weight, thermally labile
It is a chromatographic molecules. It is especially
method of separating suited to the separation
biochemical mixtures, of chiral compound
based on a highly specific stationary phase of a thin
biologic interaction such layer of adsorbent like
as that between antigen alumina on a flat, inert
and antibody, enzyme
and substrate, or
receptor and ligand.
REVERSED-PHASE
CHROMATOGRAPHY
(RPC) PAPER
CHROMATOGRAPHY of
substrate.
includes any
chromatographic method involves placing a small
that uses a non-polar dot or line of sample
stationary phase. All the solution onto a strip of
mathematical and polar cellulose
experimental chromatography paper.
considerations used in