OKP for the preparation of EDTA(0.05M or 0.

1M) & Its standardization

Process Description Method Information. Machine TG:N/A Reference Number: Dept. Name & Rev. No. 00
OKP for the preparation of ASTM E200 Calibration Number: Section SOP Source:
EDTA(0.05M or 0.1 M) & Its Quality Control/IQC
standardization Chemical

Operational Key Point

EDTA Solution preparation:
1. Take about 100 ml of water in a cleaned & dried 1000 ml Volumetric flask.
2. Add about 37.2 gm of EDTA( For 0.1M) & 18.6 g of EDTA ( For 0.05M) with continues stirring & add more
about 700 ml of water mix.
3.Make up the volume 1000 ml with water. Mix the solution throughly & keep the solution for at least 1 hour &
then carry out the standardization.
Standardization Procedure:
1) Weigh accurately about 400 mg of Calcium carbonate(Previously dried at 110 degree for about 2 hours).
2) Transfer it in a cleaned & dried 500 ml conical flask & add about 10 ml of water with gently stirring to form the
slurry.
3) Dissolve the carbonate in 2 ml of 3 N HCl & shake well until it dissolves. Add 100 ml water through the inner
surface of the flask with the strring the solution.
4)Add 30 ml of 0.1 M EDTA or 0.05 M from the burette with constant stirring & add 15 ml of NaOH(1N) solution
with constant stirring. Add 300 mg Hydroxy Napthol blue solution as an indicator .
5)Add 0.1 M or 0.05 M EDTA with constant stirring & titrate it until the solution gives a blue colour.
Calculation :Calculate the molarity of EDTA Solution as follows:
M =W/100.09V
Where,
M=Molarity of EDTA Solution
W=Miligram of calcium carbonate in the sample of Calcium carbonate taken.
V= ML of EDTA Solution consumed.
Stability: Restandardize Monthly.

1. Control the room temperature At 25°C .

2. Use safety Mask when chemical is Used.
3. Put on Hand Gloves when clean & chemical used in laboratory.
4. Read and understand all the instructions mentioned in the process manual before the test.

Issue Date: Prepared By Checked By Approved By